ABSTRACT
An actinomycete that excretes inulin fructotransferase to the culture supernatant was able to produce di-d-fructofuranose 1,2':2,3' dianhydride (DFA III) from inulin, with the greatest rate of enzyme activity at 65°C and at a pH of 5.5. Through chemotaxonomic and 16S rRNA gene analysis, this strain was identified as genus Nonomuraea in the Streptosporangiaceae family. This is the first report of an inulin fructotransferase producer in this family.
Subject(s)
Actinomycetales/isolation & purification , Actinomycetales/metabolism , Hexosyltransferases/biosynthesis , Soil Microbiology , Actinomycetales/classification , Actinomycetales/genetics , DNA, Bacterial/genetics , Indonesia , Inulin/metabolism , Molecular Sequence Data , Phylogeny , RNA, Ribosomal, 16S/genetics , Sequence Analysis, DNAABSTRACT
Two isoprenylated flavanones, macatrichocarpins A and B, and two isoprenylated dihydrochalcones, macatrichocarpins C and D, have been isolated and identified from the acetone extract of the leaves of Macaranga trichocarpa. The structures of these compounds were determined based on spectroscopic data, including UV, IR, 1D and 2D NMR spectroscopic and HREIMS data. This is the first report of the presence of dihydrochalcone derivatives in the genus Macaranga.
