Reaching new lands: Updating the distribution of Angiostrongylus cantonensis in South America with the first record in Argentina.

BACKGROUND: Angiostrongylus cantonensis, commonly known as the rat lungworm, is a metastrongyloid nematode found primarily not only in tropical and subtropical regions but also in temperate areas and considered the leading cause of eosinophilic meningitis in humans. Synanthropic rodents such as Rattus norvegicus and Rattus rattus are the most frequent definitive hosts of this parasite. METHODS AND RESULTS: The presence of this parasite was detected in the pulmonary arteries of three specimens of R. norvegicus in the city of Buenos Aires representing the species' southernmost known record in natural hosts. Species confirmation was achieved through partial sequences of 18S and COI genes. By comparing the COI gene sequences with those available in GenBank through the construction of a haplotype network, we obtained that the analysed specimen presents high similarity with those reported in Japan and Southeast Asia. CONCLUSIONS: All infected rats were captured in an area surrounding a port with significant import and export activity, suggesting that A. cantonensis may have been introduced through commercial ships. Specifically, the parasite was detected in a neighbourhood with vulnerable socio-economic conditions and in a nature reserve, which exhibit biotic and abiotic characteristics conducive to sustaining high-density rat populations, scattered waste, areas of spontaneous vegetation, debris accumulation and flooded areas or lagoons offering suitable habitats for intermediate hosts such as snails. Thus, the close proximity of the port to these sites creates a favourable ecological context for the establishment of A. cantonensis. This study shows the need to conduct research to detect A. cantonensis in non-endemic areas but with the characteristics that promote its arrival and development of its life cycle in order to implement control measures to prevent expansion of this parasite and its transmission to humans and other animals.

Intra- and inter-annual variations in metal concentrations in the superficial water of a highly polluted urban basin of Argentina.

To protect ecosystems impacted by human activities and prevent their degradation, it is imperative to evaluate variations in the concentration of environmental pollutants over time. Here, we evaluated the intra- and inter-annual variations of several metals from 15 sites in the Matanza Riachuelo River basin (one of the most polluted in the world) and determined the physicochemical and meteorological parameters associated with these changes from 2008 to 2015. For this, in each site, we used Asymmetric Eigenvector Maps and Redundancy Analysis. The results highlighted temporal patterns of metal concentrations and several factors associated with them, perhaps related to the actions performed in the area since 2008. Additionally, we found that the effects of physicochemical and meteorological factors on metal concentrations were site-dependent, possibly related to the presence of different local sources of pollution or characteristics of the river in each site, such as its sediments. This approach could be applied to different scenarios (aquatic and terrestrial) and could provide a tool to help decision-makers address the harmful consequences of the continuous advance of human activities on human and ecosystem health.

A review of the diversity of Cryptosporidium in Rattus norvegicus, R. rattus and Mus musculus: What we know and challenges for the future.

The aim of this paper is to review the diversity of Cryptosporidium species and genotypes infecting synantropic rodents. A total of 27 papers published between 1990 and 2020 assed the presence of Cryptosporidium in these rodents worldwide and described 17 different species and genotypes of Cryptosporidium. A great variation in the prevalence values were observed (0-63%). The most frequent species/genotypes were Rat genotype I and IV for R. norvegicus and Rat genotype II and III R. rattus, while C. tyzzeri was for M. musculus. Cryptosporidium parvum, the second most common species after C. hominis involved in human cryptosporidiosis cases, was the third most detected Cryptosporidium species in R, norvergicus (9.4% of the positive samples) and the 3 rodent species are common host for C. muris, also recognized as zoonotic. Besides, these synanthopic rodents can harbor Cryptosporidium species whose natural hosts are cattle, bovids, pigs, other rodent species, birds and a broad range of mammals. Considering the diversity described so far, it would have a great epidemiological impact to know how the variation of Cryptosporidium species composition along urban-rural gradients is like, including synanthropic rodents, wild and domestic animals and environmental samples, and to analyze the causal factors of such variation.

Co-occurrence of and risk factors for Cryptosporidium and Giardia in brown rats from Buenos Aires, Argentina.

A rodent survey was conducted in different landscape units of the city of Buenos Aires (Argentina) to determine the prevalence of Cryptosporidium and Giardia in Rattus norvegicus and to, ultimately, assess the biotic, environmental and meteorological factors that explain the variations of the likelihood of infection for both parasites in an urban environment. The results of this study revealed a ubiquitous presence of Cryptosporidium and Giardia in R. norvegicus within an urban environment with the likelihood of infection depending on environmental and meteorological conditions for both parasites. The overall prevalence was greater for Cryptosporidium (p = 50.4%) than for Giardia (20.3%). The prevalence for both parasites separately was higher in parks compared to shantytowns and scrap metal yards. Generalized Linear Mixed Models revealed that the occurrence of these parasites separately, at an individual level, was positively related with rainfall variables and that the effect of temperature depended on the landscape unit. The similarities in the transmission modes, which are affected by common extrinsic factors, may facilitate the co-occurrence of Cryptosporidium and Giardia in urban rats. Rattus norvegicus is recognized as a good model for epidemiological studies and the results of this work suggest that, from an epidemiological point of view, the probability of contact with infectious oocysts and cysts of these parasites can be modulated through environmental management and healthy behaviour towards risk factors. The information presented here will be useful to improve the understanding of the dynamics of zoonotic diseases within urban environments and to contribute to the decision-making of new and effective prophylactic measures.

Are soft tissues of urban rats good indicators of exposure to heavy metal pollution? A study conducted in one of the most polluted basins of Buenos Aires, Argentina.

The overall goal of this work was to analyze the relationship between the concentration of lead in the kidney, liver, and stomach contents of rats captured in a polluted urban basin and the concentration of this metal in the environment, meteorological factors, and different intrinsic characteristics of the individuals. To this end, we determined the concentration of lead in the kidney, liver, and stomach contents of 133 Norway rats (Rattus norvegicus) captured in the Matanza-Riachuelo River Basin (Buenos Aires, Argentina). This basin is one of the most severe cases of water pollution in Latin America and metals like lead represent the most common chemical pollutants. Rats were trapped in nine sites with different soil and water lead concentrations, between 2014 and 2015. A positive relationship was found between lead concentration in the liver and kidney of R. norvegicus and the environmental concentration of this metal in water and soil. Although the remaining variables analyzed were also related to the lead concentration in soft tissues, they did not affect the association between the lead concentration in soft tissues and that in the environment. The lead concentration in the stomach contents was not related to any of the variables analyzed. Our results constitute the first study in an urban basin with a gradient of environmental lead concentration and suggest that the quantification of lead in the kidney and liver of rats can be used as an indicator of exposure to this metal within a large city.

Structure of parasite communities in urban environments: the case of helminths in synanthropic rodents.

Identifying patterns with sufficient predictive power is a constant challenge for ecologists to address ecological problems related to species conservation, pollution or infectious disease control. During the last years, the amounts of parasitological studies in this sense increased, but they are still scarce in urban environments. The main aim of this study was to investigate if the helminth communities of urban rodents are structured within host assembly (compound community) or they are a result of random events occurring at each individual host scale (infracommunity). A total of 203 rodents belonging to four species, Rattus rattus (Linnaeus), Rattus norvegicus (Berkenhout), Mus musculus Linnaeus and the native Oligoryzomys flavescens (Waterhouse) and captured in different landscape units of the City of Buenos Aires (industrial-residential neighbourhoods, shantytowns and parklands) were analysed. The results showed that infracommunities could be grouped according to composition and relative abundances and that they respond to the structure of the host community. Thus, the component communities defined in this study could be identified as subsets of the compound community (rodent assemblage) and infracommunities (each host) as random samples within each one. Quantitative differences among component communities were denoted by comparing the infection levels of helminths described as central species. Therefore, infracommunities of R. norvegicus and O. flavescens were the most predictable because of the high abundance of the nematodes Heterakis spumosa Schneider, 1866 and Nippostrongylus brasiliensis (Travassos, 1914), and Stilestrongylus flavescens (Sutton et Durette-Desset, 1991), respectively. Several mechanisms contribute to complexity of the structure of parasite communities, where specific parasites, definitive and intermediate hosts, and environmental and anthropogenic factors all play a role in the dynamics of the compound community.

Helminth Diversity in Synanthropic Rodents from an Urban Ecosystem.

Richness and diversity of parasites depend on a set of interrelated factors related to the characteristics of the host, the environment and the parasites itself. In the City of Buenos Aires, rodent communities vary according to landscape structure. The goal of this paper was to study the variations of helminth richness and diversity among invasive rodent species in different landscape units of the City of Buenos Aires. 73% of the rodents were parasitized with at least one of the 10 identified helminth species. Each rodent species presented its own characteristics in terms of richness, diversity and helminth composition, keeping these characteristics still occupying more than one landscape unit. The infracommunities with greater diversity corresponded to R. norvegicus due to its high values of parasitic richness, proportion of infected hosts and parasite prevalence. Instead, R. rattus and M. musculus infracommunities had lower diversity since a high percentage of them presented a unique helminth species. Within the city, the inhabitants of shantytowns would be the most exposed to zoonotic diseases transmitted by rodents due to high abundance of rodents harboring a high parasite load, including species like Hymenolepis nana and H. diminuta, recognized worldwide from a zoonotic aspect.

Environmental health education in schools as strategy for rodent control: an experience in a shantytown of Buenos Aires, Argentina.

The general aim of this study was to assess the possibility of including elements of environmental health education within the curriculum of a school located in a shantytown of Buenos Aires city, Argentina. An environmental health education campaign was designed to introduce school-aged children to the problems posed by the lack of environmental sanitation, by using rodents as indicators of environmental disorder. The methodology implemented consisted of a lecture and two practical activities where the participating children were the evaluators of their neighborhood environment, recording the environmental factors that indicate direct or indirect presence of rodents and carrying out a survey about rodents among their neighbors. To assess the impact of the activities, an anonymous questionnaire was performed with the students before and after the campaign. The results showed that students were able to identify the man-made factors which favor the presence of rodents and were encouraged to propose strategies related to environmental sanitation to reduce rodent proliferation and the transmission of their parasites. This study demonstrated the feasibility of performing environmental health education campaigns in school-aged children by using practical activities to stimulate observation, participation, and comprehensive understanding of the problems posed by urban pests.