ABSTRACT
In the recently published results of the 1977 College of American Pathologists Enzymology Survey, Some anomalous results were obtained in measuring the creatine kinase (EC 2.7.3.2) activity of the samples. The cause of these anomalies was discovered to be an arginine kinase (EC 2.7.3.3) contaminant in one of the enzyme pools used to make the samples. The contaminant shows cross reactivity with creatine phosphate and is activated by dithioerythritol but not the other commonly used thiol activators.
Subject(s)
Arginine Kinase/blood , Creatine Kinase/blood , Phosphotransferases/blood , Adenylate Kinase/blood , Creatine Kinase/standards , Dithioerythritol , Enzyme Activation , Humans , Pathology, Clinical , Phosphocreatine/metabolism , Reference Standards , Societies, Medical , United StatesSubject(s)
Calcium , Magnesium , Phosphates , Urea , Chemical Phenomena , Chemistry , Diphosphates , Ribonucleotides/chemical synthesisABSTRACT
Struvite, MgNH(4)PO(4).6H(2)O, rather than apatite or amorphous calcium phosphate is precipitated when phosphate is added to seawater containing more than 0.01M NH(4)(+) ions. Struvite may have precipitated from evaporating seawater on the primitive earth, and may have been important for prebiotic phosphorylation.
