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1.
Carbohydr Polym ; 274: 118637, 2021 Nov 15.
Article in English | MEDLINE | ID: mdl-34702460

ABSTRACT

Chitin and its derivatives have anticoagulant, antimicrobial, and antioxidant properties, but the poor solubility of chitin limits its application in different fields. In this study, site-directed mutagenesis was performed to enhance the deacetylation activity of chitin deacetylases CDA from Arthrobacter (ArCE4). The mutant Mut-2-8 with Y172E/E200S/Y201W showed a 2.84- fold and 1.39-fold increase in catalytic efficiency (kcat/Km) for the deacetylation of (GluNAc)5 and α-chitin, respectively. These results demonstrated that the mutations significantly improved the activation of ArCE4 on crystalline chitin. The molecular docking study confirmed that the enhancement of catalytic efficiency is due to the extra two hydrogen bonds and one acetyl group. In summary, the activity of Mut-2-8 to insoluble chitin was significantly improved by reactional design, which is beneficial to resolve the issues of the expensive cost of the enzymes and low efficiency. Mut-2-8 exhibits potential applications in the chitosan industry.


Subject(s)
Amidohydrolases/chemistry , Arthrobacter/metabolism , Chitin/chemistry , Kinetics
2.
Prep Biochem Biotechnol ; 51(1): 28-34, 2021.
Article in English | MEDLINE | ID: mdl-32633612

ABSTRACT

Organic solvent-tolerant proteases have many applications in the synthesis of peptides. In this study, we have developed a low-cost and convenient method to produce highly concentrated organic solvent-tolerant protease. Organic solvent tolerant protease (OSP) gene from Bacillus sphaericus DS11 was cloned and expressed in Bacillus subtilis WB800. The optimum pH of the recombinant protease was 9.0. The optimum temperature of the recombinant protease was 40 °C. The recombinant protease was purified by ethanol with the yield of (87.33%). The yield of OSP enriched by ethanol was higher than that of by Ni-chelating affinity chromatography, which indicated that precipitation of the recombinant OSP with ethanol is a relatively low-cost and fast method for organic solvent -tolerant protease preparation. These results showed that this enzyme could be very useful in different industrial applications.


Subject(s)
Bacillaceae/enzymology , Bacillaceae/genetics , Bacillus subtilis/enzymology , Bacillus subtilis/genetics , Bacterial Proteins/biosynthesis , Bacterial Proteins/chemistry , Peptide Hydrolases/biosynthesis , Peptide Hydrolases/chemistry , Solvents/chemistry , Bacterial Proteins/genetics , Chemical Precipitation , Detergents/chemistry , Enzyme Stability , Ethanol/chemistry , Genes, Bacterial , Hydrogen-Ion Concentration , Peptide Hydrolases/genetics , Recombinant Proteins/isolation & purification , Temperature
3.
Int J Biol Macromol ; 152: 922-929, 2020 Jun 01.
Article in English | MEDLINE | ID: mdl-32114175

ABSTRACT

The chitin deacetylase produced by marine strain Nitratireductor aquimarinus MCDA3-3 (NaCDA) was purified by using ammonium sulfate precipitation, Q Sepharose, and Superdex column chromatography. The purified NaCDA showed 75-fold purity, 50 U/mg specific activity with 28.5% yield. The purified CDA molecular weight was about 36 kDa. The temperature and pH of the purified enzyme were suiting at 30 °C and 8.0, respectively. The NaCDA was highly stable for a wide range of temperature 4 °C-25 °C and pH 6.0-9.0. Besides, increased enzyme activity was observed by introducing metal ions mainly Sr2+, Mg2+, and Na+. The enzyme was founded inhibited by Co2+, Ba2+, and EDTA at the value of 1 mM concentrations. On the other hand, NaCDA was shown an active activity behavior toward glycol chitin and chitin oligomers with a degree of polymerization more than four, any polymer below the chain such as trimer and dimer significantly reduce in the activity rate, and inactive with N-acetylglucosamine. Interestingly, NaCDA showed a high activity rate against insoluble chitins and converting acetyls to deacetylated. The reduction of acetyls from 56.26% and 22.88% of acetyl groups from ɑ-chitin and ß-chitin, respectively. Hence, the NaCDA would be applicable in production chitosan toward mass production.


Subject(s)
Amidohydrolases/isolation & purification , Amidohydrolases/metabolism , Chitin/chemistry , Chitin/metabolism , Phyllobacteriaceae/enzymology , Solubility , Substrate Specificity
4.
Int J Biol Macromol ; 128: 603-609, 2019 May 01.
Article in English | MEDLINE | ID: mdl-30710583

ABSTRACT

If natural proteases are used in organic synthesis, they are often inactivated or give a low rate of reaction in non-aqueous or aqueous-organic media. Therefore, to reveal the molecular mechanism governing the stability of proteases in organic solvents and increase protease stability in those systems is of intriguing interest. In the present study, the activity and conformational changes of an organic solvent-stable protease (OSP) from Bacillus sphaericus DS11 in different concentrations of methanol were investigated by measuring fluorescence, UV-Vis spectra, circular dichroism (CD), and conducting molecular dynamics (MD) simulations. The OSP expanded with increasing methanol concentration. The methanol molecules were able to enter into the OSP, leading to microenvironmental changes around the aromatic amino acids. More hydrophobic groups were exposed to the solvents at high methanol concentrations, and the original hydrophobic interaction in the protein decreased, thus resulting in the secondary and tertiary structure change in the OSP. Our results provide helpful insight into the molecular mechanism of the OSP tolerance to organic solvent and indicate directions for future work to design and engineer proteases that are stable at high organic solvent concentrations.


Subject(s)
Bacillus/enzymology , Methanol/chemistry , Peptide Hydrolases/chemistry , Solvents/chemistry , Solvents/pharmacology , Water/chemistry , Hydrophobic and Hydrophilic Interactions , Molecular Dynamics Simulation , Protein Structure, Secondary/drug effects
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