Out-of-Band Fiber-Optic Time and Frequency Transfer Using Asymmetric and Symmetric Opto-Electronic Repeaters.

Signal repeaters for fiber-optic communication can be realized with back-to-back connected transceivers. This configuration can provide high gain ( ≈ 30 dB) at low cost, and the needed semiconductor lasers and modulators can be realized for practically any relevant wavelength. Unfortunately, for time and frequency (TF) transfer the uncorrelated wavelength drifts in the transceiver lasers can compromise transfer stability, and device replacement due to failure may result in large time offsets that have to be measured via global navigation satellite services (GNSS). This work demonstrates that good results can nevertheless be obtained with standard telecom dense wavelength-division multiplexing (DWDM) transceivers over long time periods (years). More importantly, a simple wavelength-symmetric repeater is proposed that can be used to cancel the detrimental effects of wavelength drifts and which lessens the need for link recalibrations after transceiver replacements. In a proof-of-concept test setup, timing drift due to wavelength drift of a repeater laser is reduced by approximately two orders of magnitude.

Temperature and pH dependence of DNA ejection from archaeal lemon-shaped virus His1.

The archaeal virus His1 isolated from a hypersaline environment infects an extremely halophilic archaeon Haloarcula hispanica. His1 features a lemon-shaped capsid, which is so far found only in archaeal viruses. This unique capsid can withstand high salt concentrations, and can transform into a helical tube, which in turn is resistant to extremely harsh conditions. Hypersaline environments exhibit a wide range of temperatures and pH conditions, which present an extra challenge to their inhabitants. We investigated the influence of pH and temperature on DNA ejection from His1 virus using single-molecule fluorescence experiments. The observed number of ejecting viruses is constant in pH 5 to 9, while the ejection process is suppressed at pH below 5. Similarly, the number of ejections within 15-42 °C shows only a minor increase around 25-37 °C. The maximum velocity of single ejected DNA increases with temperature, in qualitative agreement with the continuum model of dsDNA ejection.

DNA ejection from an archaeal virus--a single-molecule approach.

The translocation of genetic material from the viral capsid to the cell is an essential part of the viral infection process. Whether the energetics of this process is driven by the energy stored within the confined nucleic acid or cellular processes pull the genome into the cell has been the subject of discussion. However, in vitro studies of genome ejection have been limited to a few head-tailed bacteriophages with a double-stranded DNA genome. Here we describe a DNA release system that operates in an archaeal virus. This virus infects an archaeon Haloarcula hispanica that was isolated from a hypersaline environment. The DNA-ejection velocity of His1, determined by single-molecule experiments, is comparable to that of bacterial viruses. We found that the ejection process is modulated by the external osmotic pressure (polyethylene glycol (PEG)) and by increased ion (Mg(2+) and Na(+)) concentration. The observed ejection was unidirectional, randomly paused, and incomplete, which suggests that cellular processes are required to complete the DNA transfer.

Stroboscopic supercontinuum white-light interferometer for MEMS characterization.

We used a supercontinuum-based scanning white-light interferometer to characterize the oscillation of a MEMS device. The output of a commercially available supercontinuum light source (FiberWare Ilum II USB) was modulated to achieve stroboscopic operation. By synchronizing the modulation frequency of the source to the sample oscillation, dynamic 3-D profile measurements were recorded. These results were validated against those obtained with a white light LED setup. The measured maximum deflection of a 400×25×4 µm(3) microbridge driven with 0-6.8 V sinusoidal voltage at 10 Hz was 1.42±0.03 µm (supercontinuum), which agreed with the LED measurement. The method shows promise for characterization of high-frequency MEMS devices.