ABSTRACT
Antibiotic U-64846 is a new entity with the molecular formula C18H35C1N4O9 (MW 486). It is a very water soluble, reddish solid which decomposes above 300 degrees C and which is air-sensitive. The antibiotic is produced by Streptomyces braegensis and it inhibits a variety of Gram-positive bacteria. Acidic hydrolysis gave 3,7-diaminoheptanoic acid. The antibiotic gives 1H NMR, 13C NMR, IR and UV spectra which indicate it is not closely related to known antibiotic families.
Subject(s)
Anti-Bacterial Agents/isolation & purification , Streptomyces/metabolism , Amines , Animals , Anti-Bacterial Agents/pharmacology , Bacteria/drug effects , Fermentation , Gas Chromatography-Mass Spectrometry , Heptanoic Acids , Hydrolysis , Magnetic Resonance Spectroscopy , MiceABSTRACT
The new antifungal agent nitrosofungin was isolated in high yields from a mixed culture of two organisms consisting of a bacterium of the genus Alcaligenes (UC 9152) and Streptomyces plicatus UC 8272. The bacterium produces the agent, the streptomycete enhances the production by providing a precursor or an inducer. Nitrosofungin in high concentrations inhibits a broad variety of pathogenic fungi in vitro. The agent is relatively non-toxic in small laboratory animals and high blood levels are obtained after either oral or systemic administration. Nitrosofungin is only the second N-nitrosohydroxylamine isolated from microbial sources to date. It has been identified as 2-N-nitrosohydroxylamino-1-propanol, an acidic and highly water-soluble compound.
Subject(s)
Alcaligenes/growth & development , Antifungal Agents/isolation & purification , Streptomyces/growth & development , Drug Evaluation, Preclinical/methods , Drug Stability , Fermentation , Fungi/drug effects , Nitrosamines/isolation & purification , Nitrosamines/toxicity , Saccharomyces cerevisiae/drug effects , SpectrophotometryABSTRACT
Desertomycin was isolated from Streptomyces macronensis Dietz sp. nov. UC 8271. Extensive spectroscopic work led us to place desertomycin in the macrocyclic lactone family which contains monazomycin, scopafungin, primycin, azalomycin F4a and niphithricins A and B. The apparent molecular formula was determined by fast atom bombardment mass spectroscopy to be C57H109NO24 (MW = 1,191). Mild acid hydrolysis yielded mannose but contrary to published reports, glutamic acid is not a constituent of desertomycin.
