ABSTRACT
To reveal Herpes simplex virus 2 specific IgG and to determine their avidity the ELISA test-kit was constructed using recombinant protein gG2 (PSC SPC Diaproph-Med). Using this test-kit the distribution of specific antibodies with different avidity indexes was investigated in practically healthy donor samples. It is possible to use the mentioned ELISA test-kit for confirmation of primary infection, and also for differentiation of primary infection, chronic disease and virus reactivation. Thus, this ELISA test-kit could be an additional tool in herpetic infection diagnosis.
Subject(s)
Antibodies, Viral , Antibody Affinity/immunology , Herpes Genitalis/diagnosis , Herpesvirus 2, Human/physiology , Immunoglobulin G , Antibodies, Viral/immunology , Chronic Disease , Cloning, Molecular , Enzyme-Linked Immunosorbent Assay , Escherichia coli , Herpes Genitalis/immunology , Herpes Genitalis/virology , Humans , Immunoglobulin G/immunology , Plasmids , Reagent Kits, Diagnostic , Recombinant Proteins/genetics , Recombinant Proteins/immunology , Severity of Illness Index , Virus ActivationABSTRACT
The authors present up-to-date information concerning different inducers of different interferon types in vivo and in vitro. Interactions between the structure and interferon-inducing activity of high and low molecular weight inducers are in the focus of this paper. There are also a lot of data, concerning different biological properties of interferon-inducing compounds. Different aspects of inducers use as antiviral and immunomodulating drugs are also discussed.
Subject(s)
Interferon Inducers , Animals , Dose-Response Relationship, Drug , Humans , In Vitro Techniques , Interferon Inducers/administration & dosage , Interferon Inducers/chemistry , Interferon Inducers/pharmacology , Interferons/analysis , Molecular Weight , Structure-Activity Relationship , Virus Diseases/immunology , Virus Diseases/metabolism , Virus Diseases/therapyABSTRACT
The paper deals with the study of production of tumour necrosis factor (TNF), the key mediator of inflammation and immune responses of the organism, under experimental and staphylococcus infection (Staphylococcus aureus, st. 209) on mice of C57B16 line. It is established that staphylococcus infection of animals lead to TNF induction. Dynamics of this cytokinin synthesis by peritoneal macrophages and splenocytes under the infection of mice with S. aureus in different doses differed in time and as to absolute values. In animals infected by staphylococcus in a dose of LD30 the TNF production was high as compared to the control for 72 h after the infection and was normalized on the 4-6th day of the experiment. When using staphylococcus in a dose of LD50, spontaneous production of this cytokinin was higher than that in the control for the whole period of observation.
