ABSTRACT
A new antibiotic designated MM 14201 has been detected in a culture of Streptomyces sp. NCIB 11813. Methods for the production and purification of MM 14201 are described. Biological evaluation has shown it has broad spectrum antibacterial activity being most effective against Serratia and Pseudomonas sp. Structural studies are reported which have demonstrated MM 14201 is a new epoxyquinone derivative.
Subject(s)
Anti-Bacterial Agents/isolation & purification , Streptomyces/growth & development , Bacteria/drug effects , Drug Evaluation, Preclinical , Fermentation , Fungi/drug effects , Haemophilus influenzae/drug effects , Microbial Sensitivity Tests , Quinones/isolation & purification , Quinones/toxicity , Streptococcus pyogenes/drug effectsABSTRACT
The screening of soil actinomyces for beta-lactamase inhibitors is described. Using a plate test a number of strains of Streptomyces were found to produce beta-lactamase inhibitory activity designated olivanic acid complex. Factors affecting the production of this complex by Streptomyces olivaceus ATCC 21379 are reported. The complex showed antibacterial activity and also inhibited a number of different types of beta-lactamase in a progressive manner. Certain ampicillin-resistant bacteria are rendered sensitive to ampicillin in the presence of olivanic acid complex at a concentration which alone did not inhibit growth.
Subject(s)
Anti-Bacterial Agents/biosynthesis , Streptomyces/metabolism , Anti-Bacterial Agents/pharmacology , Fermentation , Time Factors , beta-Lactamase Inhibitors , beta-Lactams/analysis , beta-Lactams/biosynthesis , beta-Lactams/pharmacologySubject(s)
Amidohydrolases/antagonists & inhibitors , Anti-Bacterial Agents/pharmacology , Bacteria/drug effects , beta-Lactamase Inhibitors , beta-Lactams/pharmacology , Anti-Bacterial Agents/isolation & purification , Enterobacteriaceae/drug effects , Streptomyces/metabolism , beta-Lactams/isolation & purificationABSTRACT
Production of chlorflavonin, a new antifungal antibiotic, by strains of Aspergillus candidus is described. Two wild strains of the fungus had distinctly different chlorflavonin-producing capabilities. One strain produced 25 mug of chlorflavonin per ml per 4 to 5 days in a pilot scale fermentor with stirring, using a medium containing corn steep liquor and glucose. Production of antibiotic was favored by high rates of agitation-aeration. Crude chlorflavonin was extracted from the whole brew with a hydrocarbon solvent and then purified by recrystallization from benzene and petroleum ether. The overall yield from fermentation brew to pure product was 50%.
