ABSTRACT
The culture of pelagic marine invertebrates, especially the ctenophore Mnemiopsis leidyi, has been demonstrated in past studies dating back to the 1960s; however, the mass culture of delicate pelagic invertebrates has remained elusive. By using a pair of acrylic tubes and enabling water diffusion between them, we have been able to reliably and cost effectively mass culture several genera of ctenophores (Pleurobrachia, Hormiphora, Bolinopsis, Mnemiopsis and Leucothea), one species of siphonophore (Nanomia) and one species of larvacean (Oikopleura). The simple, compact method is effective enough to support two permanent exhibits of ctenophores at the Monterey Bay Aquarium while minimizing live food culture requirements with the potential to support further investigation of pelagic marine invertebrate ontogeny, ecology and genomics.
ABSTRACT
This study provides the first measurement of microplastic abundance and distribution in surface waters and sediments in Tampa Bay, FL. Microplastic concentrations in discrete water samples ranged from 0.25 to 7.0 particles/L with an average of 0.94 (±0.52) particles/L. Samples taken with a 330⯵m plankton net had 1.2-18.1 particles/m3 with an average of 4.5 (±2.3) particles/m3. Discrete samples were 200 times higher than net samples, suggesting substantial losses or undersampling with the net. For both discrete and plankton tow samples, there were no significant differences in concentrations between stations or regions. Intense rainfall events in the summer always preceded samples with substantially higher counts. Most (>75%) microplastics were fibers. Using an average value of 1 particle/L, Tampa Bay contains ~4 billion microplastic particles. Surface sediments had an average of 280 (±290) particles/kg, ranging from 30 to 790 particles/kg. Highest concentrations of microplastics were found in sediments close to industrial sources; lowest values in Middle and Lower Tampa Bay are consistent with shorter residence times.