ABSTRACT
OBJECTIVES: The aim of the study was to molecularly characterize third-generation cephalosporin-resistant Klebsiella pneumoniae isolated from bloodstream infections in Denmark in 2018 using whole-genome sequencing (WGS) data, and to compare these isolates to the most common clones detected in 2006 and 2008. METHODS: Sixty-two extended-spectrum beta-lactamase (ESBL)/AmpC-producing K. pneumoniae isolates from Danish blood cultures from 2018 were analysed using WGS to obtain multilocus sequence typing (MLST), core genome MLST (cgMLST), resistance profile and phylogeny. These were compared to the most common ESBL K. pneumoniae clones detected in 2006 and 2008. RESULTS: The most common ESBL clone was ST15 CTX-M-15, the DHA-1 enzyme was the most common in AmpC isolates, and the OXA-48-like group was the most common carbapenemase. Thirty-nine different sequence types (STs) were found, with the most frequent being ST14, ST15 and ST37, accounting for 24% of the isolates. The isolates were subdivided into 55 complex types (CTs) of which 49 were singletons, with the most frequent being ST14-CT2080. Two of the CTX-M-15-producing isolates from 2018 belonged to the ST15-CT105/CT3078 clone, which was first detected in 2006. CONCLUSIONS: The ESBL/AmpC K. pneumoniae isolates detected in Danish blood cultures belonged to many different types. No dominant clones were circulating in Danish hospitals, but the ST15-CT105/CT3078 CTX-M-15 K. pneumoniae clone was seen 13 years after its first detection.
Subject(s)
Klebsiella Infections , Sepsis , Anti-Bacterial Agents/pharmacology , Denmark/epidemiology , Humans , Klebsiella Infections/epidemiology , Klebsiella pneumoniae/genetics , Multilocus Sequence Typing , beta-Lactamases/geneticsABSTRACT
Endogenous panophthalmitis due to Clostridium septicum (C. septicum) is a rare, but life-threatening condition. There is a known association between infection and malignancy. Our patient presented with panophthalmitis - with visual loss, proptosis, raised intraocular pressure and air bubble in the anterior chamber. C. septicum was found in cultures of pus. He was treated with antibiotics and surgery, and recovered. Further investigations showed carcinoma of the colon. This case stresses the need for urgent treatment and subsequent investigation for occult malignancy.
