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Development of a triplex real-time PCR assay for detection of Panton-Valentine leukocidin toxin genes in clinical isolates of methicillin-resistant Staphylococcus aureus.
McDonald, Ryan R; Antonishyn, Nick A; Hansen, Toni; Snook, Laelie A; Nagle, Evelyn; Mulvey, Michael R; Levett, Paul N; Horsman, Greg B.
J Clin Microbiol ; 43(12): 6147-9, 2005 Dec.
Article in English | MEDLINE | ID: mdl-16333116

ABSTRACT

Community-associated methicillin-resistant Staphylococcus aureus harboring Panton-Valentine leukocidin (PVL) genes is an emerging pathogen. A novel real-time PCR assay for identification of MRSA isolates containing PVL was developed. The PVL assay was used in a triplex format allowing simultaneous amplification of mecA, nuc, and PVL genes in 614 clinical isolates. This assay facilitates the rapid identification of PVL-positive isolates of MRSA.


Subject(s)
Methicillin Resistance , Polymerase Chain Reaction/methods , Staphylococcus aureus/drug effects , Bacterial Toxins , Community-Acquired Infections/microbiology , DNA, Bacterial/analysis , Exotoxins , Humans , Leukocidins , Soft Tissue Infections/microbiology , Staphylococcal Skin Infections/microbiology , Staphylococcus aureus/genetics , Staphylococcus aureus/isolation & purification
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