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1.
Sensors (Basel) ; 14(3): 4074-85, 2014 Feb 27.
Article in English | MEDLINE | ID: mdl-24583970

ABSTRACT

A field demonstration and longevity assessment for long-term monitoring of the explosive 2,4,6-trinitrotoluene (TNT) in a marine environment using an anti-TNT microfluidic immunosensor is described. The TNT immunosensor is comprised of a microfluidic device with 39 parallel microchannels (2.5 cm × 250 µm × 500 µm, L × W × D) fabricated in poly(methylmethacrylate) (PMMA), then chemically functionalized with antibodies possessing a high affinity for TNT. Synthesized fluorescence reporter complexes used in a displacement-based assay format were used for TNT identification. For field deployment the TNT immunosensor was configured onto a submersible moored steel frame along with frame controller, pumps and TNT plume generator and deployed pier side for intermittent plume sampling of TNT (1h increments). Under varying current and tidal conditions trace levels of TNT in natural seawater were detected over an extended period (>18 h). Overnight operation and data recording was monitored via a web interface.


Subject(s)
Biosensing Techniques/instrumentation , Ecosystem , Explosive Agents/analysis , Immunoassay/instrumentation , Seawater/chemistry , Trinitrotoluene/analysis , Calibration , Fluorescence , Microfluidics , Time Factors , Water Movements
2.
Appl Opt ; 45(15): 3593-604, 2006 May 20.
Article in English | MEDLINE | ID: mdl-16708106

ABSTRACT

Relationships and variability of bio-optical properties in coastal waters are investigated. Optical proxies indicate that these coastal waters are optically complex and highly variable and are categorized as follows: (1) relatively clear and dominated by high index of refraction, biogenic particles, (2) more turbid, consisting of mostly inorganic particles and little phytoplankton, (3) extremely turbid with high concentrations of inorganic particles, and (4) more turbid and dominated by biogenic particles. We present a method, alternative to traditional remote-sensing algorithms, of classifying coastal waters [the Spectral Angle Mapper (SAM)] and utilize the SAM to successfully isolate plume conditions in time series of downwelling irradiance and total absorption coefficient. We conclude with a discussion of the use of the SAM for coastal management operations.

4.
Phys Rev Lett ; 61(2): 135-137, 1988 Jul 11.
Article in English | MEDLINE | ID: mdl-10039041
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