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1.
Free Radic Biol Med ; 49(12): 1978-88, 2010 Dec 15.
Article in English | MEDLINE | ID: mdl-20920579

ABSTRACT

Previously, it was suggested that the release of nuclearly formed ADP-ribose polymers or ADP-ribosylated proteins could be responsible for the cytosolic and mitochondrial effects of poly(ADP-ribose) polymerase (PARP)-1 activation in oxidative stress. In this report, we provide a novel alternative mechanism. We found that reactive oxygen species-activated PARP-1 regulated the activation of JNK and p38 mitogen-activated protein kinases (MAPKs) because inhibition of PARP-1 by pharmacons, small interfering RNA silencing of PARP-1 expression, or the transdominant expression of enzymatically inactive PARP-1 resulted in the inactivation of these MAPKs. This regulation was achieved by increased expression and enlarged cytoplasmic localization of MAPK phosphatase-1 (MKP-1) upon PARP-1 inhibition in oxidative stress because changes in MKP-1 expression were reflected in the phosphorylation states of JNK and p38. Furthermore, we found that in MKP-1-silenced cells, PARP inhibition was unable to exert its protective effect, indicating the pivotal roles of JNK and p38 in mediating the oxidative-stress-induced cell death as well as that of increased MKP-1 expression in mediating the protective effect of PARP inhibition. We suggest that regulation of a protein that can directly influence cytoplasmic signaling cascades at the expression level represents a novel mechanism for the cytoplasmic action of PARP-1 inhibition.


Subject(s)
Dual Specificity Phosphatase 1/genetics , Poly(ADP-ribose) Polymerases/metabolism , p38 Mitogen-Activated Protein Kinases/metabolism , Cell Line , Dual Specificity Phosphatase 1/metabolism , Enzyme Activation , Enzyme Inhibitors/pharmacology , Humans , Hydrogen Peroxide/pharmacology , Mitogen-Activated Protein Kinase 8/metabolism , Mitogen-Activated Protein Kinase 9/metabolism , Oxidants/pharmacology , Oxidative Stress , Phenanthrenes/pharmacology , Phosphorylation , Poly (ADP-Ribose) Polymerase-1 , Poly(ADP-ribose) Polymerase Inhibitors , Protein Transport , RNA Interference , Up-Regulation
2.
Mol Cell Biochem ; 321(1-2): 155-64, 2009 Jan.
Article in English | MEDLINE | ID: mdl-18975057

ABSTRACT

We studied cardioprotective as well as Akt and extracellular signal-activated kinase (ERK) activating effect of a Ca(2+) antagonist and a beta-adrenergic receptor blocker during ischemia-reperfusion, and compared these properties of the substances with that of a poly(ADP-ribose) polymerase (PARP) inhibitor used as a positive control throughout the experiments. Langendorff-perfused isolated rat hearts were subjected to 25 min global ischemia followed by 45 min reperfusion, and recovery of energy metabolism as well as functional cardiac parameters were monitored. Although to varying extents, all substances improved recovery of creatine phosphate, ATP, intracellular pH, and reutilization of inorganic phosphate. These favorable changes were accompanied by improved recovery of heart function parameters and reduced infarct size. In addition and again to varying extents, all studied substances decreased oxidative damage (lipid peroxidation and protein oxidation), and activated Akt, glycogen synthase kinase (GSK)-3beta, and ERK1/2. Correlation between cardioprotective and kinase activating effectivity of the compounds proved to be statistically significant. Physiological significance of these kinase activations was established by demonstrating that inhibition of Akt by LY294002 and ERK1/2 by PD98059 compromised the cardioprotective effect of all the substances studied. In conclusion, we demonstrated for the first time that activation of phosphatidylinositol-3-kinase (PI-3K)-Akt and ERK2 pathways significantly contributed to cardioprotective effects of a Ca(2+) antagonist and a beta-adrenergic receptor blocker. Furthermore, we found a strong correlation between cardioprotective and kinase-activating potencies of the substances studied (Verapamil, Metoprolol and two PARP inhibitors), which indicated the potentiality of these kinases as drug-targets in the therapy of ischemic heart disease.


Subject(s)
Adrenergic beta-Antagonists/metabolism , Calcium Channel Blockers/metabolism , Cardiotonic Agents/metabolism , Extracellular Signal-Regulated MAP Kinases/metabolism , Myocardium/metabolism , Proto-Oncogene Proteins c-akt/metabolism , Reperfusion Injury/metabolism , Animals , Benzimidazoles/metabolism , Enzyme Activation , Enzyme Inhibitors/metabolism , Humans , Hydrogen-Ion Concentration , Lipid Peroxidation , Male , Metoprolol/metabolism , Myocardial Infarction/metabolism , Myocardial Infarction/pathology , Myocardium/pathology , Oxidation-Reduction , Phosphates/metabolism , Rats , Rats, Wistar , Reactive Oxygen Species/metabolism , Reperfusion Injury/pathology , Signal Transduction/physiology , Verapamil/metabolism
3.
Orv Hetil ; 148(50): 2365-70, 2007 Dec 16.
Article in Hungarian | MEDLINE | ID: mdl-18055360

ABSTRACT

INTRODUCTION: Carotid artery stenting has become a possible treatment of significant carotid stenosis. The risk of stent occlusion and restenosis might be increased by abnormal rheological conditions amplified platelet aggregation and free radical production during the operation. AIMS: The aim of this study was to assess the changes of the rheologic parameters, platelet aggregation, and oxidative stress after endovascular treatment of carotid stenosis. METHODS: 18 patients (11 men, ages 68 +/- 9 years and 7 women, ages 62 +/- 8 years) suffering from significant carotid stenosis and treated with carotid endovascular intervention were examined. Alteration in hemorrheological parameters as well as epinephrine-, ADP-, and collagen-induced platelet aggregation were evaluated. Oxidative stress was characterized by the determination of catalase activity. The measurements were carried out directly before and after the procedure and 1, 2, 5 days and 1 month following the intervention. Preceding the operation the patients were administered a maximum dose (300 mg) of clopidogrel. RESULTS: The hematocrit, the plasma fibrinogen concentration (Pfc) and whole blood-, and plasma viscosity (Wbv and Pv) decreased significantly immediately after stenting ( p < 0.001). By the fifth day following the intervention the Pfc, Wdv, Pv, red blood cell (Rbc) aggregation and ADP-induced platelet aggregation increased significantly ( p < 0.0001) compared to values measured after the procedure. At 1 month follow-up these parameters, excepting Wbv, decreased significantly compared to measurements made on the 5th day. On the other hand, catalase activity showed significant elevation by the end of the first month. CONCLUSION: Hemorrheological parameters and platelet aggregation showed specific changes following carotid stenting. Abnormal changes of the rheological conditions and increasing platelet activation are the most pronounced in the first week following stenting, which may lead to the stent's early occlusion. Oxidative stress production returned to baseline levels only by the end of the first month.


Subject(s)
Carotid Stenosis/physiopathology , Carotid Stenosis/surgery , Free Radicals/metabolism , Hemorheology , Platelet Activation , Stents , Aged , Carotid Stenosis/metabolism , Clopidogrel , Female , Fibrinogen/metabolism , Follow-Up Studies , Hematocrit , Humans , Male , Middle Aged , Oxidative Stress , Platelet Aggregation , Platelet Aggregation Inhibitors/therapeutic use , Recurrence , Risk Factors , Ticlopidine/analogs & derivatives , Ticlopidine/therapeutic use , Time Factors
4.
Free Radic Biol Med ; 41(5): 835-48, 2006 Sep 01.
Article in English | MEDLINE | ID: mdl-16895804

ABSTRACT

In ischemia-reperfusion injuries, elevated calcium and reactive oxygen species (ROS) induce mitochondrial permeability transition (mPT), which plays a pivotal role in mediating damages and cell death. Inhibition of mPT decreases necrotic cell death; however, during reperfusion, the continuous production of ROS may contribute to the temporary opening of the pore and thus the onset of the delayed apoptotic cell death. Based on amiodarone structure, we developed the first SOD-mimetic mPT inhibitor (HO-3538) that can eliminate ROS in the microenvironment of the permeability pore. In isolated mitochondria, HO-3538 inhibited mPT and the release of proapoptotic mitochondrial proteins. It had a ROS scavenging effect and antiapoptotic effect in a cardiomyocyte line and it diminished release of mitochondrial proapoptotic proteins. Furthermore, HO-3538 significantly enhanced the recovery of mitochondrial energy metabolism and functional cardiac parameters; decreased infarct size, lipid peroxidation, and protein oxidation; and suppressed necrotic as well as apoptotic cell death pathways in Langendorff-perfused hearts. In these respects it was somewhat superior to its two constituents, amiodarone and a pyrrol-derivative free radical scavenger. These data suggest that the SOD-mimetic mPT inhibitors are ideal candidates for drug development for the alleviation of postinfarct myocardial injuries.


Subject(s)
Amiodarone/analogs & derivatives , Apoptosis , Ischemia/pathology , Necrosis , Superoxide Dismutase/metabolism , Amiodarone/pharmacology , Animals , Cytochromes c/metabolism , Humans , Jurkat Cells , Magnetic Resonance Spectroscopy , Mice , Mitochondria/metabolism , Myocardial Infarction/pathology , Necrosis/pathology , Rats , Rats, Wistar , Reperfusion Injury
5.
J Mol Cell Cardiol ; 41(1): 149-59, 2006 Jul.
Article in English | MEDLINE | ID: mdl-16716347

ABSTRACT

The inhibition of glycogen synthase kinase-3beta (GSK-3beta) via phosphorylation by Akt or protein kinase C (PKC), or the activation of mitogen-activated protein kinase (MAPK) cascades can play a pivotal role in left ventricular remodeling following myocardial infarction. Our previous data showed that MAPK and phosphatidylinositol-3-kinase/Akt pathways could be modulated by poly(ADP-ribose)polymerase (PARP) inhibition raising the possibility that cardiac hypertrophic signaling responses may be favorably influenced by PARP inhibitors. A novel PARP inhibitor (L-2286) was tested in a rat model of chronic heart failure following isoproterenol-induced myocardial infarction. Subsequently, cardiac hypertrophy and interstitial collagen deposition were assessed; additionally, mitochondrial enzyme activity and the phosphorylation state of GSK-3beta, Akt, PKC and MAPK cascades were monitored. PARP inhibitor (L-2286) treatment significantly reduced the progression of postinfarction heart failure attenuating cardiac hypertrophy and interstitial fibrosis, and preserving the integrity of respiratory complexes. More importantly, L-2286 repressed the hypertrophy-associated increased phosphorylation of panPKC, PKC alpha/betaII, PKC delta and PKC epsilon, which could be responsible for the activation of the antihypertrophic GSK-3beta. This work provides the first evidence that PARP inhibition beneficially modulates the PKC/GSK-3beta intracellular signaling pathway in a rat model of chronic heart failure identifying a novel drug target to treat heart failure.


Subject(s)
Glycogen Synthase Kinase 3/metabolism , Heart Failure/prevention & control , Myocardial Infarction/complications , Piperidines/pharmacology , Poly(ADP-ribose) Polymerase Inhibitors , Protein Kinase C/metabolism , Quinazolines/pharmacology , Ventricular Remodeling , Animals , Cardiomegaly/prevention & control , Collagen Type III/drug effects , Collagen Type III/metabolism , Electrocardiography , Enzyme Inhibitors/pharmacology , Glycogen Synthase Kinase 3 beta , Heart Failure/chemically induced , Heart Failure/metabolism , Isoproterenol/adverse effects , Male , Mitogen-Activated Protein Kinases/drug effects , Mitogen-Activated Protein Kinases/metabolism , Myocardial Infarction/metabolism , Myocardial Infarction/physiopathology , Natriuretic Peptide, Brain/blood , Natriuretic Peptide, Brain/drug effects , Phosphorylation , Poly(ADP-ribose) Polymerases/metabolism , Rats , Rats, Sprague-Dawley , Signal Transduction
6.
J Biol Chem ; 280(42): 35767-75, 2005 Oct 21.
Article in English | MEDLINE | ID: mdl-16115861

ABSTRACT

According to the classical view, the cytoprotective effect of inhibitors of poly(ADP-ribose)polymerase (PARP) in oxidative stress was based on the prevention of NAD+ and ATP depletion, thus the attenuation of necrosis. Our previous data on PARP inhibitors in an inflammatory model suggested that PARP-catalyzed ADP-ribosylations may affect signaling pathways, which can play a significant role in cell survival. To clarify the molecular mechanism of cytoprotection, PARP activity was inhibited pharmacologically by suppressing PARP-1 expression by a small interfering RNA (siRNA) technique or by transdominantly expressing the N-terminal DNA-binding domain of PARP-1 (PARP-DBD) in cultured cells. Cell survival, activation of the phosphatidylinositol 3-kinase (PI3-kinase)/Akt system, and the preservation of mitochondrial membrane potential were studied in hydrogen peroxide-treated WRL-68 cells. Our data showed that suppression of the single-stranded DNA break-induced PARP-1 activation by pharmacological inhibitor, siRNA, or by the transdominant expression of PARP-DBD protected cells from oxidative stress and induced the phosphorylation and activation of Akt. Furthermore, prevention of Akt activation by inhibiting PI3-kinase counteracted the cytoprotective effect of PARP inhibition. Microscopy data showed that PARP inhibition-induced Akt activation was responsible for protection of mitochondria in oxidative stress because PI3-kinase inhibitors diminished the protective effect of PARP inhibition. Similarly, Src kinase inhibitors, which decrease Akt phosphorylation, also counteracted the protection of mitochondrial membrane potential supporting the pivotal role of Akt in cytoprotection. These data together with the finding that PARP inhibition in the absence of oxidative stress induced the phosphorylation and activation of Akt indicate that PARP inhibition-induced Akt activation is dominantly responsible for the cytoprotection in oxidative stress.


Subject(s)
Mitochondria/metabolism , Oxidative Stress , Poly(ADP-ribose) Polymerases/metabolism , Proto-Oncogene Proteins c-akt/metabolism , Acetylcysteine/metabolism , Blotting, Western , Cell Line , Cell Survival , Enzyme Activation , Enzyme Inhibitors/pharmacology , Humans , Hydrogen Peroxide/pharmacology , Liver/metabolism , Membrane Potentials , Microscopy, Fluorescence , Models, Biological , Necrosis , Phosphatidylinositol 3-Kinases/metabolism , Phosphorylation , Protein Structure, Tertiary , RNA, Small Interfering/metabolism , Time Factors
7.
J Pharmacol Exp Ther ; 315(1): 273-82, 2005 Oct.
Article in English | MEDLINE | ID: mdl-15951400

ABSTRACT

Blocking poly(ADP-ribosyl)ation of nuclear proteins protects the heart from ischemia-reperfusion injury. In addition, activation of Akt and mitogen-activated protein kinase (MAPK) cascades also plays a pivotal role in the survival of cardiomyocytes during ischemia-reperfusion; however, the potential interplay between these pathways is yet to be elucidated. We therefore tested the hypothesis whether poly(ADP-ribose) polymerase (PARP) inhibition can modulate Akt and MAPK signaling of ischemic-reperfused rat hearts. A novel PARP inhibitor, L-2286 [2-[(2-piperidin-1-yletil)thio]quinazolin-4(3H)-one] was administered during ischemia-reperfusion in Langendorff perfused rat hearts and in isoproterenol-induced myocardial infarction. Thereafter, the cardiac energy metabolism, oxidative damage, and the phosphorylation state of Akt and MAPK cascades were monitored. L-2286 exerted significant protective effect against ischemia-reperfusion-induced myocardial injury in both experimental models. More importantly, L-2286 facilitated the ischemia-reperfusion-induced activation of Akt, extracellular signal-regulated kinase, and p38-MAPK in both isolated hearts and in vivo cardiac injury. By contrast, isoproterenol-induced rapid c-Jun N-termainal kinase activation was repressed by L-2286. Here, we provide evidence for the first time that PARP inhibition beneficially modulates the cardiac Akt and MAPK signaling in ex vivo and in vivo ischemia-reperfusion models. We therefore propose that this novel mechanism may contribute to the cardioprotective properties of PARP inhibitors.


Subject(s)
Enzyme Inhibitors/pharmacology , Heart/drug effects , Isoproterenol/toxicity , Mitogen-Activated Protein Kinases/physiology , Piperidines/pharmacology , Poly(ADP-ribose) Polymerase Inhibitors , Protective Agents/pharmacology , Quinazolines/pharmacology , Animals , Energy Metabolism/drug effects , Hydrogen Peroxide/pharmacology , Lipid Peroxidation/drug effects , Male , Myocardium/metabolism , Perfusion , Phosphorylation , Proteins/metabolism , Rats , Rats, Sprague-Dawley
8.
Bioorg Med Chem ; 13(7): 2629-36, 2005 Apr 01.
Article in English | MEDLINE | ID: mdl-15755662

ABSTRACT

Several amiodarone analogues were synthesized varying the 2-substituent on the benzofuran ring and diethylaminoethyl side chain of phenolether by introducing 2,2,5,5-tetramethyl-2,5-dihydro-1H-pyrrole and 1,2,5,6-tetrahydropyridine nitroxides or their amino or hydroxylamino precursors. The new compounds were screened on isolated mitochondria and perfused heart and their toxicity was evaluated on WRL-68 liver cells and H9C2 cardiomyocytes. Most of the newly synthesized derivatives exerted uncoupling effect on the mitochondrial oxidative phosphorilation at higher concentrations, compared to amiodarone and one of the modified amiodarone analogues showed an effect similar to that of amiodarone on the mitochondrial permeability transition and on restoring of mitochondrial high-energy phosphate metabolites in perfused hearts. This amiodarone analogue can be new leading compound among the experimental amiodarone analogues with the same or enhanced efficiency of amiodarone, but with less side effects.


Subject(s)
Amiodarone/chemical synthesis , Amiodarone/pharmacology , Intracellular Membranes/drug effects , Magnetics , Mitochondria/drug effects , Amiodarone/chemistry , Animals , Cell Line , Cell Survival/drug effects , Cells, Cultured , Energy Metabolism/drug effects , Hepatocytes/drug effects , Humans , Mice , Molecular Structure , Myocardial Reperfusion Injury/drug therapy , Myocardial Reperfusion Injury/prevention & control , Myocytes, Cardiac/drug effects , Permeability/drug effects , Rats , Rats, Wistar
9.
Biochem Pharmacol ; 66(11): 2263-72, 2003 Dec 01.
Article in English | MEDLINE | ID: mdl-14609750

ABSTRACT

Cardioprotective effect of a free radical-scavenging compound (HO-3073) was examined during ischaemia-reperfusion (IR) in isolated heart perfusion system and its influence on the pro-survival Akt signalling pathway was addressed. Rat hearts were perfused according to the Langendorff method and subjected to a global 25-min ischaemia and 15, 45 and 90-min reperfusion either untreated or treated with HO-3073 (2, 5 and 10 microM) and/or wortmannin (100 nM, inhibitor of phosphatidylinositol-3-kinase). HO-3073 facilitated the recovery of myocardial energy metabolism as assessed by 31P NMR spectroscopy (creatine phosphate recovery in reperfusion was 76+/-5%, while in untreated hearts 32+/-4%). Functional performance of the hearts followed by a left ventricular balloon manometer was also markedly improved by HO-3073 administration (recovery of rate-pressure product related to normoxia was 47+/-3%, while in untreated hearts 12+/-3%). HO-3073 diminished the infarct size measured by TTC staining (29+/-6% as opposed to 64+/-7% in untreated ischaemia-reperfusion). HO-3073 also significantly attenuated lipid peroxidation (thiobarbituric acid reactive substances) and protein oxidation (protein carbonyl content) compared to untreated hearts. HO-3073 enhanced the ischaemia-reperfusion-triggered phosphorylation of Akt-1 (activation) and glycogen synthase kinase-3 beta (inactivation) as evidenced by Western blot analysis. Wortmannin co-administration neutralised the beneficial effects of HO-3073 on cardiac energetics, contractile function, infarct size, as well as Akt signalling. Our results first display that a radical-scavenging molecule possesses the ability to intensify the pro-survival functioning of phosphatidylinositol-3-kinase/Akt pathway, which is presumed to play an additive role in the cardioprotective properties of HO-3073.


Subject(s)
Cardiotonic Agents/pharmacology , Protein Serine-Threonine Kinases , Proto-Oncogene Proteins/metabolism , Pyridines/pharmacology , Reperfusion Injury/enzymology , Animals , Cardiotonic Agents/chemistry , Cardiotonic Agents/therapeutic use , Enzyme Activation/drug effects , Enzyme Activation/physiology , Free Radical Scavengers/chemistry , Free Radical Scavengers/pharmacology , Free Radical Scavengers/therapeutic use , Male , Myocardial Ischemia/drug therapy , Myocardial Ischemia/enzymology , Proto-Oncogene Proteins c-akt , Pyridines/chemistry , Rats , Rats, Wistar , Reperfusion Injury/drug therapy , Signal Transduction/drug effects
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