Growth response of Listeria monocytogenes NCTC 7973 in two different media at four incubation temperatures.

Listeria monocytogenes NCTC 7973 was cultivated in tryptose phosphate broth (TPB) and tryptone soya broth (TSB) at four different temperatures: 4 degrees, 20 degrees, 30 degrees and 37 degrees C. Cells grown in TSB at 20 degrees C displayed maximum absorbance over cells grown in TPB at the same temperature. Moreover, this observation was further confirmed by dry mass determination and viable count. We determined that cells grown in TSB at 20 degrees C exhibited significant enhanced growth as compared to cells grown in TPB at the same temperature, hence TSB was found to be the medium of choice for maximum biomass production.