Understanding the mechanisms behind the antibacterial activity of magnesium hydroxide nanoparticles against sulfate-reducing bacteria in sediments.

Nanomaterials, with their small size, surface characteristics, and antibacterial properties, are extensively employed across environmental, energy, biomedical, agricultural, and other industries. This study examined the antibacterial efficacy of magnesium hydroxide (Mg(OH)2) nanoparticles (NPs) against sulfate-reducing bacteria (SRB) within sediments. The inhibitory effects of two types of Mg(OH)2 NPs with distinct particle sizes (20.3 and 29.6 nm) and concentrations (0-10.0 mg/mL) were examined under optimal treatment conditions. The antibacterial mechanisms of Mg(OH)2 NPs through direct contact and dissolution effects were determined. The results revealed a correlation between the concentration, particle size, and inhibitory activity, with the smallest NPs (20.3 nm) at the highest concentration (10.0 mg/mL) substantially reducing SRB counts from 8.77 ± 0.18 to 6.48 ± 0.13 log10 colony forming units/mL after 6 h treatment. Treatment with high concentrations of Mg(OH)2 NPs induced cellular damage, reduced intracellular lactate dehydrogenase activity, and elevated intracellular catalase activity and H2O2 content, suggesting that the contact effect of NPs stimulated SRB. This leads to oxidative stress response and structural damage to the cell membrane, which has emerged as the primary driver of the antibacterial action of Mg(OH)2 NPs. This study presents a novel nanomaterial that can inhibit and control SRB in natural sedimentary environments.

Expressive 3D Facial Animation Generation Based on Local-to-global Latent Diffusion.

3D Facial animations, crucial to augmented and mixed reality digital media, have evolved from mere aesthetic elements to potent storytelling media. Despite considerable progress in facial animation of neutral emotions, existing methods still struggle to capture the authenticity of emotions. This paper introduces a novel approach to capture fine facial expressions and generate facial animations using audio synchronization. Our method consists of two key components: First, the Local-to-global Latent Diffusion Model (LG-LDM) tailored for authentic facial expressions, which can integrate audio, time step, facial expressions, and other conditions towards possible encoding of emotionally rich yet latent features in response to possibly noisy raw audio signals. The core of LG-LDM is our carefully designed Facial Denoiser Model (FDM) for aligning the local-to-global animation feature with audio. Second, we redesign an Emotion-centric Vector Quantized-Variational AutoEncoder framework (EVQ-VAE) to finely decode the subtle differences under different emotions and reconstruct the final 3D facial geometry. Our work significantly contributes to the key challenges of emotionally realistic 3D facial animation for audio synchronization and enhances the immersive experience and emotional depth in augmented and mixed reality applications. We provide a reproducibility kit including our code, dataset, and detailed instructions for running the experiments. This kit is available at https://github.com/wangxuanx/Face-Diffusion-Model.

Efficient Photon Beam Diffusion for Directional Subsurface Scattering.

Real-time subsurface scattering techniques are widely used in translucent material rendering. Among advanced methods that rely on the bidirectional scattering-surface reflectance distribution function (BSSRDF), screen space algorithms exhibit limited translucency, while existing large-distance methods are inefficient and yield poor illumination details. To address these limitations for better large-distance scattering, we develop a novel algorithm by extending the photon beam diffusion (PBD) model within the light view and screen space. Unlike surface irradiance in prior methods, we incorporate the refracted beam in the medium into real-time scattering estimation, presenting a new consideration for photon beam utilization. Concretely, we store all photon beam samples in light view textures and utilize an adaptive sampling pattern for beam sample selection in large filtering kernel sizes. This can reduce the sample count based on surface attributes. In screen space, virtual sources are derived from samples to estimate PBD contributions, with an approximation that preserves boundary conditions. To avoid possible overestimation, we implement correction factors that scale contributions, effectively aligning our results with path-tracing references. Through these reformulations, our efficient PBD generates results closest to references among existing methods. The experiments accurately represent better front-face illumination details and backlit translucency effects, while significantly accelerating performance compared to previous large-distance methods.

TalkingStyle: Personalized Speech-Driven 3D Facial Animation with Style Preservation.

It is a challenging task to create realistic 3D avatars that accurately replicate individuals' speech and unique talking styles for speech-driven facial animation. Existing techniques have made remarkable progress but still struggle to achieve lifelike mimicry. This paper proposes "TalkingStyle", a novel method to generate personalized talking avatars while retaining the talking style of the person. Our approach uses a set of audio and animation samples from an individual to create new facial animations that closely resemble their specific talking style, synchronized with speech. We disentangle the style codes from the motion patterns, allowing our method to associate a distinct identifier with each person. To manage each aspect effectively, we employ three separate encoders for style, speech, and motion, ensuring the preservation of the original style while maintaining consistent motion in our stylized talking avatars. Additionally, we propose a new style-conditioned transformer decoder, offering greater flexibility and control over the facial avatar styles. We comprehensively evaluate TalkingStyle through qualitative and quantitative assessments, as well as user studies demonstrating its superior realism and lip synchronization accuracy compared to current state-of-the-art methods. To promote transparency and further advancements in the field, we also make the source code publicly available at https://github.com/wangxuanx/TalkingStyle.

Fluid Inverse Volumetric Modeling and Applications from Surface Motion.

In this study, we devise a framework for volumetrically reconstructing fluid from observable, measurable free surface motion. Our innovative method amalgamates the benefits of deep learning and conventional simulation to preserve the guiding motion and temporal coherence of the reproduced fluid. We infer surface velocities by encoding and decoding spatiotemporal features of surface sequences, and a 3D CNN is used to generate the volumetric velocity field, which is then combined with 3D labels of obstacles and boundaries. Concurrently, we employ a network to estimate the fluid's physical properties. To progressively evolve the flow field over time, we input the reconstructed velocity field and estimated parameters into the physical simulator as the initial state. Our approach yields promising results for both synthetic fluid generated by different fluid solvers and captured real fluid. The developed framework naturally lends itself to a variety of graphics applications, such as 1) effective reproductions of fluid behaviors visually congruent with the observed surface motion, and 2) physics-guided re-editing of fluid scenes. Extensive experiments affirm that our novel method surpasses state-of-the-art approaches for 3D fluid inverse modeling and animation in graphics.

A Unified Particle-Based Solver for Non-Newtonian Behaviors Simulation.

In this article, we present a unified framework to simulate non-Newtonian behaviors. We combine viscous and elasto-plastic stress into a unified particle solver to achieve various non-Newtonian behaviors ranging from fluid-like to solid-like. Our constitutive model is based on a Generalized Maxwell model, which incorporates viscosity, elasticity and plasticity in one non-linear framework by a unified way. On the one hand, taking advantage of the viscous term, we construct a series of strain-rate dependent models for classical non-Newtonian behaviors such as shear-thickening, shear-thinning, Bingham plastic, etc. On the other hand, benefiting from the elasto-plastic model, we empower our framework with the ability to simulate solid-like non-Newtonian behaviors, i.e., visco-elasticity/plasticity. In addition, we enrich our method with a heat diffusion model to make our method flexible in simulating phase change. Through sufficient experiments, we demonstrate a wide range of non-Newtonian behaviors ranging from viscous fluid to deformable objects. We believe this non-Newtonian model will enhance the realism of physically-based animation, which has great potential for computer graphics.

Single-cell dissection of the multicellular ecosystem and molecular features underlying microvascular invasion in HCC.

BACKGROUND AND AIMS: Microvascular invasion (MVI) is a crucial pathological hallmark of HCC that is closely associated with poor outcomes, early recurrence, and intrahepatic metastasis following surgical resection and transplantation. However, the intricate tumor microenvironment and transcriptional programs underlying MVI in HCC remain poorly understood. APPROACH AND RESULTS: We performed single-cell RNA sequencing of 46,789 individual cells from 10 samples of MVI+ (MVI present) and MVI- (MVI absent) patients with HCC. We conducted comprehensive and comparative analyses to characterize cellular and molecular features associated with MVI and validated key findings using external bulk, single-cell, and spatial transcriptomic datasets coupled with multiplex immunofluorescence assays. The comparison identified specific subtypes of immune and stromal cells critical to the formation of the immunosuppressive and pro-metastatic microenvironment in MVI+ tumors, including cycling T cells, lysosomal associated membrane protein 3+ dendritic cells, triggering receptor expressed on myeloid cells 2+ macrophages, myofibroblasts, and arterial i endothelial cells. MVI+ malignant cells are characterized by high proliferation rates, whereas MVI- malignant cells exhibit an inflammatory milieu. Additionally, we identified the midkine-dominated interaction between triggering receptor expressed on myeloid cells 2+ macrophages and malignant cells as a contributor to MVI formation and tumor progression. Notably, we unveiled a spatially co-located multicellular community exerting a dominant role in shaping the immunosuppressive microenvironment of MVI and correlating with unfavorable prognosis. CONCLUSIONS: This study provides a comprehensive single-cell atlas of MVI in HCC, shedding light on the complex multicellular ecosystem and molecular features associated with MVI. These findings deepen our understanding of the underlying mechanisms driving MVI and provide valuable insights for improving clinical diagnosis and developing more effective treatment strategies.

FineStyle: Semantic-Aware Fine-Grained Motion Style Transfer with Dual Interactive-Flow Fusion.

We present FineStyle, a novel framework for motion style transfer that generates expressive human animations with specific styles for virtual reality and vision fields. It incorporates semantic awareness, which improves motion representation and allows for precise and stylish animation generation. Existing methods for motion style transfer have all failed to consider the semantic meaning behind the motion, resulting in limited controls over the generated human animations. To improve, FineStyle introduces a new cross-modality fusion module called Dual Interactive-Flow Fusion (DIFF). As the first attempt, DIFF integrates motion style features and semantic flows, producing semantic-aware style codes for fine-grained motion style transfer. FineStyle uses an innovative two-stage semantic guidance approach that leverages semantic clues to enhance the discriminative power of both semantic and style features. At an early stage, a semantic-guided encoder introduces distinct semantic clues into the style flow. Then, at a fine stage, both flows are further fused interactively, selecting the matched and critical clues from both flows. Extensive experiments demonstrate that FineStyle outperforms state-of-the-art methods in visual quality and controllability. By considering the semantic meaning behind motion style patterns, FineStyle allows for more precise control over motion styles. Source code and model are available on https://github.com/XingliangJin/Fine-Style.git.

ZetaDesign: an end-to-end deep learning method for protein sequence design and side-chain packing.

Computational protein design has been demonstrated to be the most powerful tool in the last few years among protein designing and repacking tasks. In practice, these two tasks are strongly related but often treated separately. Besides, state-of-the-art deep-learning-based methods cannot provide interpretability from an energy perspective, affecting the accuracy of the design. Here we propose a new systematic approach, including both a posterior probability and a joint probability parts, to solve the two essential questions once for all. This approach takes the physicochemical property of amino acids into consideration and uses the joint probability model to ensure the convergence between structure and amino acid type. Our results demonstrated that this method could generate feasible, high-confidence sequences with low-energy side conformations. The designed sequences can fold into target structures with high confidence and maintain relatively stable biochemical properties. The side chain conformation has a significantly lower energy landscape without delegating to a rotamer library or performing the expensive conformational searches. Overall, we propose an end-to-end method that combines the advantages of both deep learning and energy-based methods. The design results of this model demonstrate high efficiency, and precision, as well as a low energy state and good interpretability.

Interactive Virtual Ankle Movement Controlled by Wrist sEMG Improves Motor Imagery: An Exploratory Study.

Virtual reality (VR) techniques can significantly enhance motor imagery training by creating a strong illusion of action for central sensory stimulation. In this study, we establish a precedent by using surface electromyography (sEMG) of contralateral wrist movement to trigger virtual ankle movement through an improved data-driven approach with a continuous sEMG signal for fast and accurate intention recognition. Our developed VR interactive system can provide feedback training for stroke patients in the early stages, even if there is no active ankle movement. Our objectives are to evaluate: 1) the effects of VR immersion mode on body illusion, kinesthetic illusion, and motor imagery performance in stroke patients; 2) the effects of motivation and attention when utilizing wrist sEMG as a trigger signal for virtual ankle motion; 3) the acute effects on motor function in stroke patients. Through a series of well-designed experiments, we have found that, compared to the 2D condition, VR significantly increases the degree of kinesthetic illusion and body ownership of the patients, and improves their motor imagery performance and motor memory. When compared to conditions without feedback, using contralateral wrist sEMG signals as trigger signals for virtual ankle movement enhances patients' sustained attention and motivation during repetitive tasks. Furthermore, the combination of VR and feedback has an acute impact on motor function. Our exploratory study suggests that the sEMG-based immersive virtual interactive feedback provides an effective option for active rehabilitation training for severe hemiplegia patients in the early stages, with great potential for clinical application.

Exploring invertebrate indicators of ecosystem health by focusing on the flow transitional zones in a large, shallow eutrophic lake.

The river-lake transitional zone provides a unique environment for the biological community and can reduce pollution inputs in lake ecosystems from their catchments. To explore environmental conditions with high purification potential in Lake Taihu and indicator species, we examined the river-to-lake changes in water and sediment quality and benthic invertebrate communities in the transitional zone of four regions. The spatial variations in the environment and invertebrate community observed in this study followed the previously reported patterns in Taihu; the northern and western regions were characterized by higher nutrient concentrations in water, higher heavy metal concentrations in sediment, and higher total invertebrate density and biomass dominated by pollution-tolerant oligochaetes and chironomids. Although nutrient concentrations were low and transparency was high in the eastern region, the taxon richness was the lowest there, which disagreed with the previous findings and might be due to a poor cover of macrophytes in this study. The river-to-lake change was large in the southern region for water quality and the invertebrate community. Water circulation induced by strong wind-wave actions in the lake sites of the southern region is assumed to have promoted photosynthetic and nutrient uptake activities and favored invertebrates that require well-aerated conditions such as polychaetes and burrowing crustaceans. Invertebrates usually adapted to brackish and saline environments are suggested to be indicators of a well-circulated environment with active biogeochemical processes and a less eutrophic state in Taihu, and wind-wave actions are key to maintaining such a community and natural purifying processes.

MPMNet: A Data-Driven MPM Framework for Dynamic Fluid-Solid Interaction.

High-accuracy, high-efficiency physics-based fluid-solid interaction is essential for reality modeling and computer animation in online games or real-time Virtual Reality (VR) systems. However, the large-scale simulation of incompressible fluid and its interaction with the surrounding solid environment is either time-consuming or suffering from the reduced time/space resolution due to the complicated iterative nature pertinent to numerical computations of involved Partial Differential Equations (PDEs). In recent years, we have witnessed significant growth in exploring a different, alternative data-driven approach to addressing some of the existing technical challenges in conventional model-centric graphics and animation methods. This paper showcases some of our exploratory efforts in this direction. One technical concern of our research is to address the central key challenge of how to best construct the numerical solver effectively and how to best integrate spatiotemporal/dimensional neural networks with the available MPM's pressure solvers. In particular, we devise the MPMNet, a hybrid data-driven framework supporting the popular and powerful Material Point Method (MPM), to combine the comprehensive properties of MPM in numerically handling physical behaviors ranging from fluid to deformable solids and the high efficiency of data-driven models. At the architectural level, our MPMNet comprises three primary components: A data processing module to describe the physical properties by way of the input fields; A deep neural network group to learn the spatiotemporal features; And an iterative refinement process to continue to reduce possible numerical errors. The goal of these special technical developments is to aim at involved numerical acceleration while preserving physical accuracy, realizing efficient and accurate fluid-solid interactions in a data-driven fashion. The extensive experimental results verify that our MPMNet can tremendously speed up the computation compared with the popular numerical methods as the complexity of interaction scenes increases while better retaining the numerical accuracy.

Graph Diffusion Convolutional Network for Skeleton Based Semantic Recognition of Two-Person Actions.

Graph Convolutional Networks (GCNs) have successfully boosted skeleton-based human action recognition. However, existing GCN-based methods mostly cast the problem as separated person's action recognition while ignoring the interaction between the action initiator and the action responder, especially for the fundamental two-person interactive action recognition. It is still challenging to effectively take into account the intrinsic local-global clues of the two-person activity. Additionally, message passing in GCN depends on adjacency matrix, but skeleton-based human action recognition methods tend to calculate the adjacency matrix with the fixed natural skeleton connectivity. It means that messages can only travel along a fixed path at different layers of the network or in different actions, which greatly reduces the flexibility of the network. To this end, we propose a novel graph diffusion convolutional network for skeleton based semantic recognition of two-person actions by embedding the graph diffusion into GCNs. At technical fronts, we dynamically construct the adjacency matrix based on practical action information, so that we can guide the message propagation in a more meaningful way. Simultaneously, we introduce the frame importance calculation module to conduct dynamic convolution, so that we can avoid the negative effect caused by the traditional convolution, wherein the shared weights may fail to capture key frames or be affected by noisy frames. Besides, we comprehensively leverage the multidimensional features related to joints' local visual appearances, global spatial relationship and temporal coherency, and for different features, different metrics are designed to measure the similarity underlying the corresponding real physical law of the motions. Moreover, extensive experiments and comprehensive evaluations on four public large-scale datasets (NTU-RGB+D 60, NTU-RGB+D 120, Kinetics-Skeleton 400, and SBU-Interaction) demonstrate that our method outperforms the state-of-the-art methods.

How does digital village construction influences carbon emission? The case of China.

Taking 30 provinces in China from 2011 to 2020 as a research sample, this paper empirically tests the impact of digital village construction on carbon emissions. This study found that there is an "inverted U" curve relationship between digital rural construction and rural carbon emissions. Agricultural planting structure and agricultural technology efficiency are important ways for digital village construction to reduce agricultural carbon emissions. The study also found that the higher the level of economic development, the stronger the carbon emission reduction effect of digital village construction. In addition, there are also significant differences in the carbon emission reduction effect of digital village construction in regions with different environmental regulation intensities. Finally, in terms of the relationship between digital economic activities and carbon emission reduction, the research conclusions of this paper have important implications.

Salient Object Detection via Dynamic Scale Routing.

Recent research advances in salient object detection (SOD) could largely be attributed to ever-stronger multi-scale feature representation empowered by the deep learning technologies. The existing SOD deep models extract multi-scale features via the off-the-shelf encoders and combine them smartly via various delicate decoders. However, the kernel sizes in this commonly-used thread are usually "fixed". In our new experiments, we have observed that kernels of small size are preferable in scenarios containing tiny salient objects. In contrast, large kernel sizes could perform better for images with large salient objects. Inspired by this observation, we advocate the "dynamic" scale routing (as a brand-new idea) in this paper. It will result in a generic plug-in that could directly fit the existing feature backbone. This paper's key technical innovations are two-fold. First, instead of using the vanilla convolution with fixed kernel sizes for the encoder design, we propose the dynamic pyramid convolution (DPConv), which dynamically selects the best-suited kernel sizes w.r.t. the given input. Second, we provide a self-adaptive bidirectional decoder design to accommodate the DPConv-based encoder best. The most significant highlight is its capability of routing between feature scales and their dynamic collection, making the inference process scale-aware. As a result, this paper continues to enhance the current SOTA performance. Both the code and dataset are publicly available at https://github.com/wuzhenyubuaa/DPNet.

Neurophysiological and Subjective Analysis of VR Emotion Induction Paradigm.

The ecological validity of emotion-inducing scenarios is essential for emotion research. In contrast to the classical passive induction paradigm, immersive VR fully engages the psychological and physiological components of the subject, which is considered an ecologically valid paradigm for studying emotion. Several studies investigate the emotional responses to different VR tasks or games using subjective scales. However, little research regards VR as an eliciting material, especially when systematically analyzing emotional processes in VR from a neurophysiological perspective. To fill this gap and scientifically evaluate VR's ability to be used as an active method for emotion elicitation, we investigate the dynamic relationship between explicit information (subjective evaluations) and implicit information (objective neurophysiological data). A total of 28 participants are enlisted to watch eight VR videos while their SAM/IPQ scores and EEG data are recorded simultaneously. In ecologically valid scenarios, the subjective results demonstrate that VR has significant advantages for evoking emotion in arousal-valence. This conclusion is backed by our examination of objective neurophysiological evidence that VR videos effectively induce high-arousal emotions. In addition, we obtain features of critical channels and frequency oscillations associated with emotional valence, thereby validating previous research in more lifelike circumstances. In particular, we discover hemispheric asymmetry in the occipital region under high and low emotional arousal, which adds to our understanding of neural features and the dynamics of emotional arousal. As a result, we successfully integrate EEG and VR to demonstrate that VR is more pragmatic for evoking natural feelings and is beneficial for emotional research. Our research has set a precedent for new methodologies of using VR induction paradigms to acquire a more reliable explanation of affective computing.

Foveated Stochastic Lightcuts.

Foveated rendering provides an idea for accelerating rendering algorithms without sacrificing the perceived rendering quality in virtual reality applications. In this paper, we propose a foveated stochastic lightcuts method to render high-quality many-lights illumination effects in high perception-sensitive regions. First, we introduce a spatiotemporal-luminance based lightcuts generation method to generate lightcuts with different accuracy for different visual perception-sensitive regions. Then we propose a multi-resolution light samples selection method to select the light sample for each node in the lightcuts more efficiently. Our method supports full-dynamic scenes containing over 250k dynamic light sources and dynamic diffuse/specular/glossy objects. It provides frame rates up to 110fps for high-quality many-lights illumination effects in high perception-sensitive regions of the HVS in VR HMDs. Compared with the state-of-the-art stochastic lightcuts method using the same rendering time, our method achieves smaller mean squared errors in the fovea and periphery. We also conduct user studies to prove that the perceived quality of our method has a high visual similarity with the results of the ground truth rendered by using the stochastic lightcuts with 2048 light samples per pixel.

Hierarchical anatomical structure-aware based thoracic CT images registration.

Accurate thoracic CT image registration remains challenging due to complex joint deformations and different motion patterns in multiple organs/tissues during breathing. To combat this, we devise a hierarchical anatomical structure-aware based registration framework. It affords a coordination scheme necessary for constraining a general free-form deformation (FFD) during thoracic CT registration. The key is to integrate the deformations of different anatomical structures in a divide-and-conquer way. Specifically, a deformation ability-aware dissimilarity metric is proposed for complex joint deformations containing large-scale flexible deformation of the lung region, rigid displacement of the bone region, and small-scale flexible deformation of the rest region. Furthermore, a motion pattern-aware regularization is devised to handle different motion patterns, which contain sliding motion along the lung surface, almost no displacement of the spine and smooth deformation of other regions. Moreover, to accommodate large-scale deformation, a novel hierarchical strategy, wherein different anatomical structures are fused on the same control lattice, registers images from coarse to fine via elaborate Gaussian pyramids. Extensive experiments and comprehensive evaluations have been executed on the 4D-CT DIR and 3D DIR COPD datasets. It confirms that this newly proposed method is locally comparable to state-of-the-art registration methods specializing in local deformations, while guaranteeing overall accuracy. Additionally, in contrast to the current popular learning-based methods that typically require dozens of hours or more pre-training with powerful graphics cards, our method only takes an average of 63 s to register a case with an ordinary graphics card of RTX2080 SUPER, making our method still worth promoting. Our code is available at https://github.com/heluxixue/Structure_Aware_Registration/tree/master.

Nonfinite-modality data augmentation for brain image registration.

Brain image registration is fundamental for brain medical image analysis. However, the lack of paired images with diverse modalities and corresponding ground truth deformations for training hinder its development. We propose a novel nonfinite-modality data augmentation for brain image registration to combat this. Specifically, some available whole-brain segmentation masks, including complete fine brain anatomical structures, are collected from the actual brain dataset, OASIS-3. One whole-brain segmentation mask can generate many nonfinite-modality brain images by randomly merging some fine anatomical structures and subsequently sampling the intensities for each fine anatomical structure using random Gaussian distribution. Furthermore, to get more realistic deformations as the ground truth, an improved 3D Variational Auto-encoder (VAE) is proposed by introducing the intensity-level reconstruction loss and the structure-level reconstruction loss. Based on the generated images and trained improved 3D VAE, a new Synthetic Nonfinite-Modality Brain Image Dataset (SNMBID) is created. Experiments show that pre-training on SNMBID can improve the accuracy of registration. Notably, SNMBID can be a landmark for evaluating other brain registration methods, and the model trained on the SNMBID can be a baseline for the brain image registration task. Our code is available at https://github.com/MangoWAY/SMIBID_BrainRegistration.

Effect of remediation reagents on bacterial composition and ecological function in black-odorous water sediments.

Black-odorous urban water bodies and sediments pose a serious environmental problem. In this study, we conducted microcosm batch experiments to investigate the effect of remediation reagents (magnesium hydroxide and calcium nitrate) on native bacterial communities and their ecological functions in the black-odorous sediment of urban water. The dominant phyla (Proteobacteria, Actinobacteria, Chloroflexi, and Planctomycetes) and classes (Alphaproteobacteria, Betaproteobacteria, and Gammaproteobacteria, Actinobacteria, Anaerolineae, and Planctomycetia) were determined under calcium nitrate and magnesium hydroxide treatments. Functional groups related to aerobic metabolism, including aerobic chemoheterotrophy, dark sulfide oxidation, and correlated dominant genera (Thiobacillus, Lysobacter, Gp16, and Gaiella) became more abundant under calcium nitrate treatment, whereas functional genes potentially involved in dissimilatory sulfate reduction became less abundant. The relative abundance of chloroplasts, fermentation, and correlated genera (Desulfomonile and unclassified Cyanobacteria) decreased under magnesium hydroxide treatment. Overall, these results indicated that calcium nitrate addition improved hypoxia-related reducing conditions in the sediment and promoted aerobic chemoheterotrophy.