ABSTRACT
Plant height, one of the most important factors for plant architectures, is closely related with some important traits such as plant density, lodging resistance and yields.. In the present study, 201 QTLs related to plant height of soybean from database and references were analyzed. The results revealed that 15 consensus QTLs located on chromosomes 6, 7, 11, 13, and 18 were identified after physical integrations and Meta analysis using the software BioMercator 2.1. Moreover, "Overview" method was used to optimize these QTLs based on statistic analysis. Some of the valid QTL regions were narrowed down to the 0.1 cM and mapped on certain chromosomes. Furthermore, the functions of all genes located in these confidence regions were predicted and 17 candidate genes that might be related to plant height were identified. These results would lay a foundation for fine mapping of QTLs related to plant height and marker-assisted breeding in soybean.
Subject(s)
Glycine max/anatomy & histology , Glycine max/genetics , Phenotype , Quantitative Trait Loci , Computational Biology/methods , Genes, Plant , Physical Chromosome MappingABSTRACT
By using two-dimensional electrophoresis and mass spectrometry techniques, the proteomes in the tillering node of cold-resistant winter wheat Dongnongdongmai 1 and cold-susceptible winter wheat Jimai 22 before and after low temperature treatment were comparatively analyzed. It was observed that within the range of pH 4-7, there was a significant difference in the expression level of 55 protein spots in the protein profiles of tillering node before and after low temperature treatment, and 47 of the 55 protein spots were expressed in the two cultivars. After low temperature treatment, the abundance value of 23 protein spots increased, with the absolute abundance being higher in Dongnongdongmai 1 than in Jimai 22, while that of 7 protein spots decreased, with the absolute abundance being lower in Dongnongdongmai 1 than in Jimai 22. The expression level of 8 protein spots specific in Dongnongdongmai 1 was up-regulated. In all of the up-regulated proteins, their expression level in Dongnongdongmai 1 was 2.1 - 16.5 times as high as that in Jimai 22, among which, 10 protein spots had an expression level of 4 times high. In the down-regulated proteins, their expression level in Dongnongdongmai 1 was 0.1-0.4 times as high as that in Jimai 22. The differential protein spots were detected by mass spectrometry, and identified by compared with retrieval database. A total of 51 protein spots fingerprints were obtained, among which, 15.7% were of stress protein, 27.5% were of metabolism-related protein, 19.6% were signaling molecules, 9.8% were of unknown protein, and other proteins took up 27.4%. These variously expressed proteins might play an important role in the cold resistance of Dongnongdongmai 1.
Subject(s)
Cold Temperature , Plant Proteins/metabolism , Proteome/metabolism , Triticum/metabolism , Electrophoresis, Gel, Two-Dimensional , Mass Spectrometry , Triticum/classification , Triticum/geneticsABSTRACT
The antioxidant activities of water extract, methanol extract, ethyl acetate, and n-BuOH fractions of methanol extract from Lysimachia foenum-graecum Hance were investigated in this study. Various methods, such as the total antioxidant capacity measured by phosphomolybdenum method, scavenging activities towards 1,1-diphenyl-2-picryhydrazyl (DPPH) radical, superoxide anion radical, and hydroxyl radical, were established in in vitro systems. The amounts of total phenolics and total flavonoids in the extracts and fractions were also determined by spectrophotometric methods. The results showed that all the extracts and fractions exhibited antioxidant and radical-scavenging activities at different magnitudes of potency. The decreasing order of antioxidant and radical-scavenging activities among the extracts assayed through all the four methods were found to be ethyl acetate fraction>n-BuOH fraction>methanol extract>water extract. This similar order of the amounts of total phenolics and total flavonoids shows that the extent of antioxidant and radical-scavenging activities is in accordance with the amounts of phenolics and flavonoids present in extracts and fractions. The extracts of L. foenum-graecum Hance might be valuable antioxidant natural sources and seemed to be applicable in both healthy medicine and food industry.
