ABSTRACT
Forty-four aeromonads were isolated during the summer of 1994 from snails (Helix aspersa) affected or not by a newly described disease. Snta 1 rRNA gene restriction patterns (ribotyping) and nine selected conventional phenotypic tests were performed to retrospectively determine relationships among these isolates regarding the disease. Results from ribotyping were found highly discriminant in strain differentiation and suggest the possible existence of a group of Aeromonas associated with the disease. Biochemical and molecular characterizations indicated that most of these disease-associated aeromonads could belong to the phenospecies A. hydrophila. Phenospecies of aeromonads from healthy snails were distinct from those isolated from diseased snails and could be identified as A. caviae or remained undetermined. To the authors' knowledge, this is the first report of a probable pathogenic aeromonad found in snails, extending the host-pathogen spectrum of Aeromonas.
Subject(s)
Aeromonas/isolation & purification , Helix, Snails/microbiology , Aeromonas/classification , Aeromonas/genetics , Animals , Bacterial Typing Techniques/veterinary , France , Phenotype , RNA, Bacterial/genetics , RNA, Ribosomal/genetics , Retrospective Studies , SeasonsABSTRACT
One hundred and ninety strains of coagulase-negative staphylococci were isolated from goat's milk, whey and cheese at various stages of manufacture. Sixteen different coagulase negative Staphylococci (CNS) species were recovered, 3 of which were predominant: Staphylococcus simulans, Staphylococcus epidermidis and Staphylococcus xylosus. The prevalent species were recovered at least at two different stages of cheese manufacturing, suggesting a better adaptation to the environment. After 15 days of ripening, the cheeses showed lower counts of Micrococcaceae.
