Recent progress on emerging technologies for trace elements-contaminated soil remediation.

Abiotic stresses from potentially toxic elements (PTEs) have devastating impacts on health and survival of all living organisms, including humans, animals, plants, and microorganisms. Moreover, because of the rapid growing industrial activities together with the natural processes, soil contamination with PTEs has pronounced, which required an emergent intervention. In fact, several chemical and physical techniques have been employed to overcome the negative impacts of PTEs. However, these techniques have numerous drawback and their acceptance are usually poor as they are high cost, usually ineffectiveness and take longer time. In this context, bioremediation has emerged as a promising approach for reclaiming PTEs-contaminated soils through biological process using bacteria, fungus and plants solely or in combination. Here, we comprehensively reviews and critically discusses the processes by which microorganisms and hyperaccumulator plants extract, volatilize, stabilize or detoxify PTEs in soils. We also established a multi-technology repair strategy through the combination of different strategies, such as the application of biochar, compost, animal minure and stabilized digestate for stimulation of PTE remediation by hyperaccumulators plants species. The possible use of remote sensing of soil in conjunction with geographic information system (GIS) integration for improving soil bio-remediation of PTEs was discussed. By synergistically combining these innovative strategies, the present review will open very novel way for cleaning up PTEs-contaminated soils.

Alcaligenes aquatilis GTE53: Phosphate solubilising and bioremediation bacterium isolated from new biotope "phosphate sludge enriched-compost".

The isolation and identification of beneficial bacteria from the active phase of composting is considered to be a key bio-quality parameter for the assessment of the process. The aim of this work was the selection and identification of beneficial bacteria from a co-composting experiment of vegetable waste (VW), olive oil mill waste (O2MW), and phosphate sludge (PS). Phosphate-solubilizing strains were isolated from the thermophilic phase using Pikovskaya (PVK) solid medium supplemented with tricalcium phosphate Ca3(PO4) (TCP) as the sole source of phosphorus (P). Therefore, the selected isolate Alcaligenes aquatilis GTE53 was tested to tolerate abiotic stresses (different levels of temperature, variable pH, high salinity and water stress). The isolate was also assessed for indole acetic acid (IAA) and siderophores synthesis, nitrogen fixation, phenol degradation and pathogens inactivation. The quality of the co-composting process was also investigated by monitoring the physico-chemical parameters. The obtained results showed that A. aquatilis GTE53 displayed a higher solubilization index of 2.4 and was efficiently dissolved, up to 162.8 and 247.4 mg·mL-1 of inorganic phosphate from PS and phosphate rock (PR), respectively. A. aquatilis GTE53 exhibited siderophores and IAA release, along with atmospheric nitrogen fixation. In addition to that, A. aquatilis GTE53 showed a high resistance to heat and tolerance to acidic and alkaline pH, high salinity and water stress. Moreover, A. aquatilis GTE53 could degrade 99.2% of phenol from a high-concentrated medium (1100 mg·L-1 of phenol) and can inactivate the most abundant pathogens in industrial wastes: Escherichia coli, Streptococcus sp., Salmonella sp., and Fusarium oxysporum albedinis. Analysis of temperature, pH, electrical conductivity, carbon/nitrogen (C/N) ratio, indicated successful co-composting. An efficient transformation of P to the available form and a great abatement of polyphenols, were also recorded during the process. The findings of this study will help to advance the understanding of A. aquatilis GTE53 functions and will facilitate its application to promote beneficial microbial organisms during composting, thus obtaining a high-quality product.