Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 1 de 1
Filter
Add more filters










Database
Language
Publication year range
1.
Biochim Biophys Acta Mol Basis Dis ; 1866(6): 165731, 2020 06 01.
Article in English | MEDLINE | ID: mdl-32088316

ABSTRACT

Outer membrane vesicles (OMVs) are nanosized particles derived from the outer membrane of gram-negative bacteria. Oral bacterium Porphyromonas gingivalis (Pg) is known to be a major pathogen of periodontitis that contributes to the progression of periodontal disease by releasing OMVs. The effect of Pg OMVs on systemic diseases is still unknown. To verify whether Pg OMVs affect the progress of diabetes mellitus, we analyzed the cargo proteins of vesicles and evaluated their effect on hepatic glucose metabolism. Here, we show that Pg OMVs were equipped with Pg-derived proteases gingipains and translocated to the liver in mice. In these mice, the hepatic glycogen synthesis in response to insulin was decreased, and thus high blood glucose levels were maintained. Pg OMVs also attenuated the insulin-induced Akt/glycogen synthase kinase-3 ß (GSK-3ß) signaling in a gingipain-dependent fashion in hepatic HepG2 cells. These results suggest that the delivery of gingipains mediated by Pg OMV elicits changes in glucose metabolisms in the liver and contributes to the progression of diabetes mellitus.


Subject(s)
Bacterial Outer Membrane/metabolism , Gingipain Cysteine Endopeptidases/genetics , Periodontitis/genetics , Porphyromonas gingivalis/genetics , Animals , Bacterial Outer Membrane/pathology , Disease Models, Animal , Gingipain Cysteine Endopeptidases/metabolism , Glycogen Synthase Kinase 3 beta/genetics , Humans , Insulin Resistance/genetics , Liver/metabolism , Liver/microbiology , Mice , Periodontitis/microbiology , Periodontitis/pathology , Porphyromonas gingivalis/metabolism , Porphyromonas gingivalis/pathogenicity , Proto-Oncogene Proteins c-akt/genetics , Signal Transduction/genetics
SELECTION OF CITATIONS
SEARCH DETAIL
...