Atrial Fibrillation Substrate and Catheter Ablation Outcomes in MYBPC3- and MYH7-Mediated Hypertrophic Cardiomyopathy.

BACKGROUND: The effects of disease-causing MYBPC3 or MYH7 genetic variants on atrial myopathy, atrial fibrillation (AF) clinical course, and catheter ablation efficacy remain unclear. OBJECTIVES: The aim of this study was to characterize the atrial substrate of patients with MYBPC3- or MYH7-mediated hypertrophic cardiomyopathy (HCM) and its impact on catheter ablation outcomes. METHODS: A retrospective single-center study of patients with HCM who underwent genetic testing and catheter ablation for AF was performed. Patients with MYBPC3- or MYH7-mediated HCM formed the gene-positive cohort; those without disease-causative genetic variants formed the control cohort. High-density electroanatomical mapping was performed using a 3-dimensional mapping system, followed by radiofrequency ablation. RESULTS: Twelve patients were included in the gene-positive cohort (mean age 55.6 ± 9.9 years, 83% men, 50% MYBPC3, 50% MYH7, mean ejection fraction 59.3% ± 13.7%, mean left atrial [LA] volume index 51.7 ± 13.1 mL/m2, mean LA pressure 20.2 ± 5.4 mm Hg) and 15 patients in the control arm (mean age 61.5 ± 12.6 years, 60% men, mean ejection fraction 64.9% ± 5.1%, mean LA volume index 54.1 ± 12.8 mL/m2, mean LA pressure 19.6 ± 5.41 mm Hg). Electroanatomical mapping demonstrated normal voltage in 87.7% ± 5.03% of the LA in the gene-positive cohort and 94.3% ± 3.58% of the LA in the control cohort (P < 0.001). Of the abnormal regions, intermediate scar (0.1-0.5 mV) accounted for 6.33% ± 1.97% in the gene-positive cohort and 3.07% ± 2.46% in the control cohort (P < 0.01). Dense scar (<0.1 mV) accounted for 5.93% ± 3.20% in the gene-positive cohort and 2.61% ± 2.19% in the control cohort (P < 0.01). Freedom from AF at 12 months was similar between the gene-positive (75%) and control (73%) cohorts (P = 0.92), though a greater number of procedures were required in the gene-positive cohort. CONCLUSIONS: Patients with MYBPC3- or MYH7-mediated HCM undergoing AF ablation have appreciably more low-amplitude LA signals, suggestive of fibrosis. However, catheter ablation remains an effective rhythm-control strategy.

Cardiac resynchronization therapy in inotrope-dependent heart failure: a meta-analysis.

AIMS: The viability of cardiac resynchronization therapy (CRT) in inotrope-dependent heart failure (HF) has been a matter of debate. METHODS AND RESULTS: We searched Medline, EMBASE, Scopus, and the Cochrane Library until 31 December 2022. Studies were included if (i) HF patients required inotropic support at CRT implantation; (ii) patients were ≥18 years old; and (iii) they provided a clear definition of 'inotrope dependence' or 'inability to wean'. A meta-analysis was performed in R (Version 3.5.1). Nineteen studies comprising 386 inotrope-dependent HF patients who received CRT (mean age 64.4 years, 76.9% male) were included. A large majority survived until discharge at 91.1% [95% confidence interval (CI): 81.2% to 97.6%], 89.3% were weaned off inotropes (95% CI: 77.6% to 97.0%), and mean discharge time post-CRT was 7.8 days (95% CI: 3.9 to 11.7). After 1 year of follow-up, 69.7% survived (95% CI: 58.4% to 79.8%). During follow-up, the mean number of HF hospitalizations was reduced by 1.87 (95% CI: 1.04 to 2.70, P < 0.00001). Post-CRT mean QRS duration was reduced by 29.0 ms (95% CI: -41.3 to 16.7, P < 0.00001), and mean left ventricular ejection fraction increased by 4.8% (95% CI: 3.1% to 6.6%, P < 0.00001). The mean New York Heart Association (NYHA) class post-CRT was 2.7 (95% CI: 2.5 to 3.0), with a pronounced reduction of individuals in NYHA IV (risk ratio = 0.27, 95% CI: 0.18 to 0.41, P < 0.00001). On univariate analysis, there was a higher prevalence of males (85.7% vs. 40%), a history of left bundle branch block (71.4% vs. 30%), and more pronounced left ventricular end-diastolic dilation (274.3 ± 7.2 vs. 225.9 ± 6.1 mL). CONCLUSIONS: CRT appears to be a viable option for inotrope-dependent HF, with some of these patients seeming more likely to respond.

Outcomes of mitral TEER in non-responders to cardiac resynchronization therapy: A systematic review and Meta-analysis.

BACKGROUND: Secondary mitral regurgitation (MR) worsens in 10-15 % of heart failure (HF) patients receiving cardiac resynchronization therapy (CRT). Transcatheter edge-to-edge repair (TEER) with Mitra-Clip (Abbot Vascular, Santa Clara, CA, USA) therapy is associated with improved survival and decreased rates of hospitalization for HF in selected patients with secondary MR. Data on TEER outcomes in CRT-non-responders are limited. The purpose of this meta-analysis was to evaluate outcomes of mitral TEER with Mitra-Clip in CRT-non-responders. METHODS: Cochrane, Scopus, MEDLINE, and EMBASE were searched for studies discussing outcomes of Mitra-Clip in CRT non-responders. Two reviewers were independently involved in screening studies and extracting relevant data. Individual study incidence rate estimates underwent logit transformation to calculate the weighted summary proportion under the random effect model. RESULTS: A total of eight reports met the inclusion criteria (439 patients). Mitra-Clip improved MR grade to ≤2+ in 83.8 % and 86.8 % of CRT non-responders at six months and one year, respectively. Symptomatic improvement (New York Heart Association class ≤II) was also found in 71 % and 78.1 % of CRT non-responders at six months and one year, respectively. The pooled overall incidence estimates of mortality at 30 days, 6 months, 1 year, and 2 years were 3.6 %, 9.2 %, 17.8 %, and 25.9 %, respectively. CONCLUSION: TEER with Mitra-Clip in patients with significant secondary MR who do not respond to CRT was associated with MR improvement, alleviation of symptoms, and mortality rates similar to those in the COAPT trial.

A Bibliometric Analysis of Most Cited Papers on Vesiculobullous Oral Lesions.

Aim: A well-known method for quantitatively evaluating scholarly work is bibliometric analysis. Best-cited papers raise awareness of the influential publications and patterns in the literature on a specific subject. The aim was to conduct bibliometric analysis to determine most cited articles on vesiculobullous oral lesions. This is the first study on citation analysis with respect to vesiculobullous oral lesions. Materials and Methods: A retrospective data search was explored on December 2022 using the Scopus database. The articles were evaluated, and fundamental data for bibliometric analysis was reviewed. Standard details about the author, linked organizations, publishing year, and place of origin were noted. Statistical analysis was performed using Chi-square analysis. VOSviewer software was used to determine the bibliometric network analysis for co-occurrence among coauthors and commonly used keywords. Results: A total of 344 articles published from 1971 to 2022 were included in the study. A total of 6680 citations and 19.41 citations per article were observed. The journal Archives of Dermatology received the most citation. There was a significant association between the number of citations and the journal type (open access vs. non-open access) (P < 0.05). Four to five highly related clusters with the help of VOSviewer software were found during co-occurrence network analysis. Conclusions: The top 10 articles on vesiculobullous oral lesions that received the most citations were listed in detail in the present study. This will be a valuable resource for academics, clinicians, and researchers in the fields of dermatology, general pathology, oral pathology, and oral medicine.

Trends in the development and current perspective of thermostable bacterial hemicellulases with their industrial endeavors: A review.

Hemicellulases are enzymes that hydrolyze hemicelluloses, common polysaccharides in nature. Thermophilic hemicellulases, derived from microbial strains, are extensively studied as natural biofuel sources due to the complex structure of hemicelluloses. Recent research aims to elucidate the catalytic principles, mechanisms and specificity of hemicellulases through investigations into their high-temperature stability and structural features, which have applications in biotechnology and industry. This review article targets to serve as a comprehensive resource, highlighting the significant progress in the field and emphasizing the vital role of thermophilic hemicellulases in eco-friendly catalysis. The primary goal is to improve the reliability of hemicellulase enzymes obtained from thermophilic bacterial strains. Additionally, with their ability to break down lignocellulosic materials, hemicellulases hold immense potential for biofuel production. Despite their potential, the commercial viability is hindered by their high enzyme costs, necessitating the development of efficient bioprocesses involving waste pretreatment with microbial consortia to overcome this challenge.

Artificial intelligence-enhanced electrocardiogram for arrhythmogenic right ventricular cardiomyopathy detection.

Aims: ECG abnormalities are often the first signs of arrhythmogenic right ventricular cardiomyopathy (ARVC) and we hypothesized that an artificial intelligence (AI)-enhanced ECG could help identify patients with ARVC and serve as a valuable disease-detection tool. Methods and results: We created a convolutional neural network to detect ARVC using a 12-lead ECG. All patients with ARVC who met the 2010 task force criteria and had disease-causative genetic variants were included. All case ECGs were randomly assigned in an 8:1:1 ratio into training, validation, and testing groups. The case ECGs were age- and sex-matched with control ECGs at our institution in a 1:100 ratio. Seventy-seven patients (51% male; mean age 47.2 ± 19.9), including 56 patients with PKP2, 7 with DSG2, 6 with DSC2, 6 with DSP, and 2 with JUP were included. The model was trained using 61 case ECGs and 5009 control ECGs; validated with 7 case ECGs and 678 control ECGs and tested in 22 case ECGs and 1256 control ECGs. The sensitivity, specificity, positive and negative predictive values of the model were 77.3, 62.9, 3.32, and 99.4%, respectively. The area under the curve for rhythm ECG and median beat ECG was 0.75 and 0.76, respectively. Conclusion: Our study found that the model performed well in excluding ARVC and supports the concept that the AI ECG can serve as a biomarker for ARVC if a larger cohort were available for network training. A multicentre study including patients with ARVC from other centres would be the next step in refining, testing, and validating this algorithm.

Abridgement of Microbial Esterases and Their Eminent Industrial Endeavors.

Esterases are hydrolases that contribute to the hydrolysis of ester bonds into both water-soluble acyl esters and emulsified glycerol-esters containing short-chain acyl groups. They have garnered significant attention from biotechnologists and organic chemists due to their immense commercial value. Esterases, with their diverse and significant properties, have become highly sought after for various industrial applications. Synthesized ubiquitously by a wide range of living organisms, including animals, plants, and microorganisms, these enzymes have found microbial esterases to be the preferred choice in industrial settings. The cost-effective production of microbial esterases ensures higher yields, unaffected by seasonal variations. Their applications span diverse sectors, such as food manufacturing, leather tanneries, paper and pulp production, textiles, detergents, cosmetics, pharmaceuticals, biodiesel synthesis, bioremediation, and waste treatment. As the global trend shifts toward eco-friendly and sustainable practices, industrial processes are evolving with reduced waste generation, lower energy consumption, and the utilization of biocatalysts derived from renewable and unconventional raw materials. This review explores the background, structural characteristics, thermostability, and multifaceted roles of bacterial esterases in crucial industries, aiming to optimize and analyze their properties for continued successful utilization in diverse industrial processes. Additionally, recent advancements in esterase research are overviewed, showcasing novel techniques, innovations, and promising areas for further exploration.

Gender Variation, subject dispersion and citation impact in dental research: A bibliometric analysis of publications from 2009-2021.

Aim: This study probed into the gender variation, subject dispersion and citation impact of dental research with an objective to highlight the increasing influence of female authors in the field of dentistry. Methodology: The research employed bibliometric techniques to highlight gender variation in dental research as demonstrated in the Saudi Dental Journal (SDJ), over a period of thirteen years, 2009-2021. The examination comprised SDJ publications indexed in PubMed, with citation data extracted from Google Scholar on July 2023. The analysis encompassed the progressive growth of papers, authors, and citations, the gender distribution of authors, the co-authorship structure, subject dispersion, and collaboration patterns based on affiliation, both national and international. Results: 625 SDJ publications were identified in the PubMed database from 2009 to 2021. There was a notable rise in the volume of papers and the number of authors per year. The citation impact analysis revealed that these 625 papers achieved an average of 26 citations each. Remarkably, the representation of female authors increased from 0.47 to 2.23 authors per paper during the study period. The authorship pattern largely showed single-authorship, closely followed by a three-author pattern. Almost half the papers (48%) were a collaboration between male and female authors, with female authors solely producing about 15% of papers.The topological classification of papers revealed that the most considerable number of papers were on Periodontics, with the least number focusing on Oral and Maxillofacial Surgery. Female authors prominently contributed to Pediatric Dentistry and Periodontics papers. Conclusion: The study illustrates an encouraging trend of heightened female authorship in dental research over the years. Their increasing engagement not only enriches the academic diversity but also impacts the progressive evolution of dental science, contributing to a more inclusive and balanced society.

Anatomy of the Ventricular Outflow Tracts: An Electrophysiology Perspective.

Outflow tract ventricular arrhythmias are the most common type of idiopathic ventricular arrhythmia. A systematic understanding of the outflow tract anatomy improves procedural efficacy and enables electrophysiologists to anticipate and prevent complications. This review emphasizes the three-dimensional spatial relationships between the ventricular outflow tracts using seven anatomical principles. In turn, each principle is elaborated on from a clinical perspective relevant for the practicing electrophysiologist. The developmental anatomy of the outflow tracts is also discussed and reinforced with a clinical case.

Improving the genetic potential of okra (Abelmoschus esculentus L.) germplasm to tolerate salinity stress.

Okra (Abelmoschus esculentus L.) is the most consumed vegetable worldwide with the potential for diverse ecological adaptation. However, increasing salinization and changing climatic conditions are posing serious threats to the growth, yield, and quality of okra. Therefore, to mitigate increasing soil salinization and ensure sustainable okra production under rapidly changing climatic conditions, evaluation of new okra germplasm to develop salt tolerant cultivars is direly needed. The present study was designed to evaluate the genetic resources of okra genotypes for salt tolerance at growth and reproductive phases. Based on mophological and physio-biochemical responses of plants under stress condition, genotypes were divided into salt tolerant and succeptible groups. The experiment was comprised of 100 okra genotypes and each genotype was grown under control conditions and 6.5 dS m-1 NaCl concentration in a pot having 10 kg capacity. The experiment was conducted in a completely randomized design and each treatment was replicated three times. The results showed vast genetic variability among the evaluated okra germplasm traits like days to emergence, pod length, pod diameter, plant height, stem girth, and other yield-related parameters. Correlation analysis showed a highly significant positive association among the number of leaves at first flower and plant height at first flower.Likewise, pod weight also revealed a highly significant positive relationship for pod weight plant-1, pod length, and K+: Na+. Principal Component Analysis (PCA) revealed that out of 16 principal components (PCs), five components showed more than one eigenvalue and the first six PCs contributed 67.2% of the variation. Bi-plot analysis illustrated that genotypes 95, 111, 133, 99, and 128, under salt stress conditions, exhibited both high yield per plant and salt-tolerant behavior in other yield-related traits. On the basis of all studied traits, a salt susceptible group and a salt-tolerant group were formed. The salt tolerant group comprised of 97, 68, 95, 114, 64, 99, 111, 133, 128, and 109 genotypes, whereas, the salt susceptible group contained 137, 139, 130, 94, and 125 genotypes. Salt-tolerant okra genotypes were suggested to be used in further breeding programs aimed to develop salt tolerance in okra. These insights will empower precision breeding, underscore the importance of genetic diversity, and bear the potential to address the challenges of salt-affected soils while promoting broader agricultural resilience, economic prosperity, and food security.

Unveiling the Psychedelic Journey: An Appraisal of Psilocybin as a Profound Antidepressant Therapy.

Depression, a global health concern with significant implications for suicide rates, remains challenging to treat effectively with conventional pharmacological options. The existing pharmaceutical interventions for these illnesses need daily dosing, are accompanied by various adverse effects, and may exhibit limited efficacy in certain cases. However, hope emerges from an unlikely source-Psilocybin, a natural hallucinogen found in certain mushrooms. Recently, this enigmatic compound has garnered attention for its potential therapeutic benefits in addressing various mental health issues, including depression. Psilocybin alters mood, cognition, and perception by acting on a particular subtype of serotonin receptors in the brain. It's feasible that these shifts in consciousness will promote healing development, offering a novel approach to depression management. This comprehensive review explores psilocybin, derived from specific mushrooms, and its implications in the treatment of depression. The study examines new perspectives and therapeutic possibilities surrounding psilocybin, addressing existing gaps in academic literature. It delves into its biosynthesis, unique mechanisms of action, therapeutic applications, and anti-depressive effects. By uncovering the potential of this mind-altering substance, the review aims to advance psychiatric care, offering hope to those globally affected by depression.

Level of evidence analysis of the Saudi Dental Journal: A bibliometric analysis of publications from 2012 to 2021.

Objectives: The Level of Evidence (LOE) ranking system is used to measure the methodological quality of research. This study aimed to analyze and evaluate the trends of LOEs in articles published in the Saudi Dental Journal (SDJ) between 2012 and 2021. Methodology: The bibliometric details of all articles published from 2012 to 2021 were extracted from the SDJ website. All articles, expect editorials, were included in the analysis. The articles were divided based on LOEs, dental specialties, number of authors, and centers. The citation metrics were obtained from Google Scholar, and the statistical analysis was performed using JMP Pro 15.2.0 software. Results: Five hundred twenty-two articles were selected for analysis. They had an average of 21.19 citations per article, and a growing trend in the number of articles was observed. Authors from 40 countries contributed to the articles, with the most contributions from the Kingdom of Saudi Arabia. Most articles (n = 269; 51.53%) were LOE IV and V, while a low proportion (5.56%) were LOE I articles. Aside from miscellaneous articles, periodontics composed most of the LOE I studies, followed by endodontics, and oral and maxillofacial Surgery (OMFS). Orthodontics had the highest number of LOE II studies, pediatric dentistry had the most LOE IV, and prosthodontics had the most LOE V studies. No significant correlations were found between LOE and the number of authors or centers. However, a significant correlation was found in the distribution of LOE contributed by academic institutes. Conclusion: The study results highlight that most articles were LOE IV and V, whereas nominal LOE I articles were found. Furthermore, there is a need to encourage dental scientists to carry out high-quality evidence studies. Professional dental societies can play a pivotal role in this regard.

Bibliometric Analysis of the 100 Most-Cited Articles on Periodontics in the Arab World.

AIM: Periodontics is a branch of dentistry that deals with diseases of the supporting and investing structures of the teeth including the gingiva, cementum, periodontal ligament, and alveolar bone. The current study aimed to scrutinize the bibliometric indicators of the 100 most-cited articles on periodontics contributed by authors affiliated with the Arab countries. RESEARCH METHODOLOGY: The bibliometric research method was used for the 100 most-cited articles retrieved from the Scopus database on May 9, 2023. The keywords periodontitis, periodontology, gingivitis, periodontal, gingiva, and periodontium were inserted, and then the Arab countries were selected from the country's filter. The 100 most-cited articles were accessed. The bibliometric indicators such as periodic distribution of articles, their study design, nature of research, contributed by different Arab countries, international research collaboration, and author's used keywords were analyzed. Microsoft Excel version 16 (Microsoft Corporation, Washington, United States), VOSviewer (Centre for Science and Technology Studies, Leiden University, The Netherlands), and SPSS Statistics version 27 (IBM Corp. Released 2020. IBM SPSS Statistics for Windows, Version 27.0. Armonk, NY: IBM Corp.) were used for data analysis. RESULTS: A slow progress (4.50%) was found in periodontics literature before 2000 in the Arab countries, but a significant growth (75%) was recorded during the past 10 years (January 1, 2014, to May 9, 2023). The 100 most-cited articles on periodontics by the Arab countries were published from 1995 to 2021, and these articles gained citations with an average of 92.18 citations per articles. Case-control and review studies were the preferred study designs, and periodontology and implantology were the top two subject categories. About one-third of the articles were published in the Journal of Periodontology and the Journal of Clinical Periodontology. Saudi Arabia contributed the highest number of the most-cited articles, followed by Egypt and Jordan, but the articles contributed by the United Arab Emirates were the most influential. The United States and Germany were the top-ranked countries in international research collaboration. CONCLUSION: The findings demonstrate that periodontics research increased remarkably during the past 10 years. Saudi Arabia stands not only on the topmost rank in overall research productivity in the Arab countries but also surpasses the rest of the Arab countries in the 100 most-cited articles. Saudi Arabia contributed 26 articles with the United States, while Jordan contributed the highest ratio of indigenous research.

Co-production of biohydrogen and biomethane utilizing halophytic biomass Atriplexcrassifolia by two-stage anaerobic fermentation process.

The excessive use of fossil has resulted in the drastic exhaustion of natural energy sources, leading to environmental challenges and energy crises. Owing to rising energy demand there is a dire need to shift towards renewable energies from lignocellulosic biomass. The present study assessed the co-production of biohydrogen (H2) and biomethane (CH4) by utilizing a less explored halophyte Atriplexcrassifolia. Various reaction parameters were evaluated for their effect on biohydrogen and biomethane production in batch experiments. One parameter at a time experimental strategy was chosen for production optimization. Hydrogen and methane yields along with their production rates were assessed at different incubation times, temperatures, pH, substrate concentrations, and inoculum sizes in acidogenesis and methanogenesis stages, respectively. In the first stage, maximum cumulative hydrogen production of 66 ± 0.02 mL, with hydrogen yield of 13.2 ± 0.03 mL/g, and hydrogen production rate (HPR) of 1.37 ± 0.05 mL/h was attained when the reaction mixture (5 g Atriplexcrassifolia and 10 mL pretreated sewage sludge) was processed at 37°C and pH 5.5 after 48 h of incubation. While in the second stage, maximum cumulative methane production, i.e., 343 ± 0.12 mL, methane yield (MY) of 8.5 ± 0.07 mL/mL, and methane production rate (MPR) of 0.8 ± 0.05 mL/h was achieved after 18 days of incubation of reaction mixture (40 mL of hydrogenic slurry with 80 mL inoculum) at 45°C and pH 8. Furthermore, a 51% and 24% rise in biohydrogen and biomethane production respectively were recorded when the gases were produced at these optimized reaction conditions. The results ensure halophyte Atriplexcrassifolia as an imperative renewable energy resource and proposed that effective optimization of the process further increased the coproduction of biohydrogen and biomethane.

Research Performance of Saudi Arabia in Endodontics: A Bibliometric Analysis.

Endodontics is a subdivision of dentistry that deals with diseases and injury of the soft tissues inside the teeth. The current study aimed to investigate the bibliometric properties of endodontics publications contributed by Saudi Arabia from 2010 to 2022. The quantitative bibliometric research method was applied to the meta-data retrieved from the Web of Science on December 7, 2022. The word Endodonti* was written in the main search bar, and from the year's filter, selected starting year 2010 to the date of data collection. In the first phase, to access the global publication growth of endodontics, no country/region filter was used. After getting the summary of the global intellectual landscape, we selected Saudi Arabia from the country/region filter to analyze certain characteristics of endodontics documents. Periodical growth, accessibility modes, collaboration patterns, influential institutions, frequently used sources, international research collaboration, and most-cited documents were analyzed by using Microsoft Excel (v.16) and SPSS (v.20). The authors affiliated with Brazil contributed the highest number of documents and Saudi Arabia stood in the eighth rank in endodontics research. The growing tendency was observed in Saudi Arabia from 1.29% in 2010 to 7.60% in 2022 from a global perspective. The quality of nonopen-accessed documents was higher than open-accessed as per citation impact, similarly, the documents written on international collaboration got a higher ratio of citations compared with national collaboration. King Saud University was found the most prolific institution and the Journal of Endodontics was the most preferred source of publications. The maximum research collaboration at the international level was performed with the authors of the United States. Fifteen most-cited papers obtained 21.42% of the citations. The findings show that endodontics research in Saudi Arabia has increased significantly over the years. The research collaboration at the national level has increased, which shows that teams of national researchers in endodontics have been prepared and conducting valuable research in a national context.

Burgeoning therapeutic strategies to curb the contemporary surging viral infections.

Significant efforts and initiatives were already made in the health care systems, however in the last few years; our world is facing emergences of viral infections which potentially leading to considerable challenges in terms of higher morbidity, mortality, increased and considerable financial loads on the affected populations. Over ten major epidemics or pandemics have been recorded in the twenty-first century, the ongoing coronavirus pandemic being one of them. Viruses being distinct obligate pathogens largely dependent on living beings are considered as one of the prominent causes of death globally. Although effective vaccines and antivirals have led to the eradication of imperative viral pathogens, the emergences of new viral infections as well as novel drug-resistant strains have necessitated the implementation of ingenious and efficient therapeutic approaches to treat viral outbreaks in the future. Nature being a constant source of tremendous therapeutical resources has inspired us to develop multi-target antiviral drugs, overcoming the challenges and limitations faced by pharmaceutical industry. Recent breakthroughs in the understanding of the cellular and molecular mechanisms of viral reproduction have laid the groundwork for potential treatment approaches including antiviral gene therapy relying on the application of precisely engineered nucleic acids for disabling pathogen replication. The development of RNA interference and advancements in genome manipulating tools have proven to be especially significant in this regard. In this review, we discussed mode of actions and pathophysiological events associated with the viral infections; followed by distributions, and advancement made towards the detection strategies for timely diagnosis. In the later section, current approaches to cope up the viral pathogens and their key limitations have also been elaborated. Lastly, we also explored some novel and potential targets to treat such infections, where attentions were made on next generation gene editing technologies.

Bacterial thermophilic DNA polymerases: A focus on prominent biotechnological applications.

DNA polymerases are the enzymes able to replicate the genetic information in nucleic acid. As a result, they are necessary to copy the complete genome of every living creature before cell division and sustain the integrity of the genetic information throughout the life of each cell. Any organism that uses DNA as its genetic information, whether unicellular or multicellular, requires one or more thermostable DNA polymerases to thrive. Thermostable DNA polymerase is important in modern biotechnology and molecular biology because it results in methods such as DNA cloning, DNA sequencing, whole genome amplification, molecular diagnostics, polymerase chain reaction, synthetic biology, and single nucleotide polymorphism detection. There are at least 14 DNA-dependent DNA polymerases in the human genome, which is remarkable. These include the widely accepted, high-fidelity enzymes responsible for replicating the vast majority of genomic DNA and eight or more specialized DNA polymerases discovered in the last decade. The newly discovered polymerases' functions are still being elucidated. Still, one of its crucial tasks is to permit synthesis to resume despite the DNA damage that stops the progression of replication-fork. One of the primary areas of interest in the research field has been the quest for novel DNA polymerase since the unique features of each thermostable DNA polymerase may lead to the prospective creation of novel reagents. Furthermore, protein engineering strategies for generating mutant or artificial DNA polymerases have successfully generated potent DNA polymerases for various applications. In molecular biology, thermostable DNA polymerases are extremely useful for PCR-related methods. This article examines the role and importance of DNA polymerase in a variety of techniques.