ABSTRACT
A three dimensional magnetic patterning of two cell types was realised in vitro inside an additive manufactured magnetic scaffold, as a conceptual precursor for the vascularised tissue. The realisation of separate arrangements of vascular and osteoprogenitor cells, labelled with biocompatible magnetic nanoparticles, was established on the opposite sides of the scaffold fibres under the effect of non-homogeneous magnetic gradients and loading magnetic configuration. The magnetisation of the scaffold amplified the guiding effects by an additional trapping of cells due to short range magnetic forces. The mathematical modelling confirmed the strong enhancement of the magnetic gradients and their particular geometrical distribution near the fibres, defining the preferential cell positioning on the micro-scale. The manipulation of cells inside suitably designed magnetic scaffolds represents a unique solution for the assembling of cellular constructs organised in biologically adequate arrangements.
Subject(s)
Biocompatible Materials/chemistry , Magnetite Nanoparticles/chemistry , Tissue Engineering/methods , Tissue Scaffolds/chemistry , Bone Regeneration , Computer Simulation , Human Umbilical Vein Endothelial Cells/physiology , Humans , Magnetic Fields , Materials Testing , Mesenchymal Stem Cells/physiology , Models, Biological , Models, Chemical , Nanomedicine/methods , Neovascularization, Physiologic/physiology , Osteogenesis/physiology , Proof of Concept StudyABSTRACT
The mathematical prediction of cell proliferation in porous scaffold still remains a challenge. The analysis of existing models and experimental data confirms a need for a new solution, which takes into account cells" development on the scaffold pore walls as well as some additional parameters such as the pore size, cell density in cellular layers, the thickness of the growing cell layer and others. The simulations, presented below, are based on three main approaches. The first approach takes into account multilayer cell growth on the pore walls of the scaffold. The second approach is a simulation of cell proliferation in a discrete process as a continuous one. The third one is the representation of scaffold structure as a system of cylindrical channels. Oxygen (nutrient) mass transfer is realized inside these channels. The model, described below, proposes the new solution to time dependent description of cell proliferation in porous scaffold and optimized trophical conditions for tissue development.
