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Sheep pancreatic microsomes as an alternative to the dog source for studying protein translocation.

Kaderbhai, M A; Harding, V J; Karim, A; Austen, B M; Kaderbhai, N N.

Biochem J ; 306 ( Pt 1): 57-61, 1995 Feb 15.

Article in English | MEDLINE | ID: mdl-7864829

ABSTRACT

A procedure is described for the preparation of rough membrane vesicles of endoplasmic-reticular origin from the pancreas of sheep. These isolated membranes translocate, process and glycosylate in vitro-translated heterologous proteins in a manner comparable with that exhibited by dog pancreatic microsomes.

Subject(s)

Dogs , Endoplasmic Reticulum/metabolism , Membrane Proteins , Microsomes/metabolism , Pancreas/ultrastructure , Proteins/metabolism , Serine Endopeptidases , Sheep , Animals , Biological Transport , Endopeptidases/metabolism , Endoplasmic Reticulum/ultrastructure , Escherichia coli/enzymology , Intracellular Membranes/metabolism , Intracellular Membranes/ultrastructure , Mating Factor , Microscopy, Electron , Peptides/metabolism , Protein Precursors/metabolism , Protein Sorting Signals/metabolism , Protein Sorting Signals/pharmacology , Saccharomyces cerevisiae/chemistry , beta-Lactamases/metabolism

Over-production and isolation of a precursor protein in a form ideal as a substrate for leader peptidase.

Kaderbhai, N N; Harding, V J; Kaderbhai, M A.

Biochem Soc Trans ; 23(1): 58S, 1995 Feb.

Article in English | MEDLINE | ID: mdl-7758773

Subject(s)

Alkaline Phosphatase/metabolism , Cytochromes b5/metabolism , Endopeptidases/metabolism , Membrane Proteins , Protein Precursors/metabolism , Protein Sorting Signals/metabolism , Serine Endopeptidases , Alkaline Phosphatase/biosynthesis , Alkaline Phosphatase/isolation & purification , Animals , Cloning, Molecular , Cytochromes b5/biosynthesis , Cytochromes b5/isolation & purification , Escherichia coli/enzymology , Liver/metabolism , Protein Precursors/biosynthesis , Protein Precursors/isolation & purification , Protein Sorting Signals/biosynthesis , Protein Sorting Signals/isolation & purification , Recombinant Fusion Proteins/biosynthesis , Recombinant Fusion Proteins/isolation & purification , Recombinant Fusion Proteins/metabolism , Substrate Specificity

ABSTRACT

Subject(s)

Subject(s)

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