ABSTRACT
Patients with acute myeloblastic leukaemia (AML) and chromosomic abnormalities of the 3q21;q26 region have striking dysmegakaryopoiesis and normal or increased platelet counts. Leukaemic cells ectopically express the Evi-1 gene which maps to human chromosome 3q26:q27. Thrombopoietin (TPO) has been cloned recently and shown to be the major hormone stimulating both megakaryocytopoiesis and thrombopoiesis. The TPO gene maps to human chromosome 3q26. For this report we studied four patients with typical 3q21:q26 syndrome. Karyotype analysis showed inv(3)(q21;q26) in three cases and t(3:3)(q21;q26) in one case. Although high levels of Evi-1 transcripts could be detected in mRNA isolated from the bone marrow cells of these patients by Northern blot analysis, no TPO transcripts were detectable by RT-PCR technique on the same mRNA samples. These results demonstrate that TPO gene transcription is not activated in patients with 3q26 chromosomic abnormality, and that abnormal TPO production is not responsible for the observed thrombocytosis.
Subject(s)
Chromosome Aberrations/genetics , Leukemia, Myeloid/genetics , Proto-Oncogenes , Thrombocytosis/etiology , Thrombopoietin/adverse effects , Transcription Factors , Acute Disease , Base Sequence , Blotting, Northern , Chromosome Disorders , Chromosomes, Human, Pair 3 , DNA-Binding Proteins/genetics , Gene Expression , Humans , Leukemia, Myeloid/complications , MDS1 and EVI1 Complex Locus Protein , Molecular Sequence Data , Syndrome , Thrombopoietin/geneticsABSTRACT
Time-resolved radiation exposure measurements inside the crew compartment have been made during recent Shuttle missions with the USAF Radiation Monitoring Equipment-III (RME-III), a portable four-channel tissue equivalent proportional counter. Results from the first six missions are presented and discussed. The missions had orbital inclinations ranging from 28.5 degrees to 57 degrees, and altitudes from 200-600 km. Dose equivalent rates ranged from 40-5300 micro Sv/dy. The RME-III measurements are in good agreement with other dosimetry measurements made aboard the vehicle. Measurements indicate that medium- and high-LET particles contribute less than 2% of the particle fluence for all missions, but up to 50% of the dose equivalent, depending on the spacecraft's altitude and orbital inclination. Iso-dose rate contours have been developed from measurements made during the STS-28 mission. The drift rate of the South Atlantic Anomaly (SAA) is estimated to be 0.49 degrees W/yr and 0.12 degrees N/yr. The calculated trapped proton and Galactic Cosmic Radiation (GCR) dose for the STS-28 mission were significantly lower than the measured values.
Subject(s)
Cosmic Radiation , Protons , Radiation Monitoring/instrumentation , Solar Activity , Space Flight/instrumentation , Spacecraft/instrumentation , Linear Energy Transfer , Radiation Dosage , Radiometry/instrumentationABSTRACT
Since STS-26, three large solar events have occurred during Shuttle missions; a geomagnetic storm during STS-29 and solar particle events (SPEs) during STS-28 and -34. The maximum dose to a crew attributed to an SPE was estimated to be 30 microGy (70 microSv). Time-resolved dosimetry measurements of the SPE dose during STS-28 were made using the Air Force Radiation Monitoring Equipment (RME)-III. Comparison of calculated and measured dose demonstrated a discrepancy, possibly a result of deficiencies in the geomagnetic cutoff model used. This experience demonstrates that dose from an SPE is strongly dependent on numerous factors such as orbit inclination, SPE start time, spectral parameters and geomagnetic field conditions; the exact combination of these factors is fortuitous. New sources of data and procedures are being investigated, including real-time tracking of auroral oval positions or determination of particle cutoff latitudes, for incorporation into operational Shuttle radiation support practices.
Subject(s)
Cosmic Radiation , Radiation Monitoring/statistics & numerical data , Solar Activity , Space Flight , Humans , Protons , Radiation Dosage , Radiation Monitoring/instrumentation , RadiometryABSTRACT
The geomagnetically-trapped and galactic cosmic radiation environments are two of the major sources of naturally-occurring space radiation exposure to astronauts in low earth orbit. The exposure is dependent primarily on altitude, spacecraft shielding, crew stay-times, and solar cycle effects for a 28.5 deg orbital inclination. Based on Space Shuttle experience, the calculated results of a parametric study are presented for several mission scenarios using a computerized anatomical man model and are compared with the NASA crew exposure limits for several critical body organs.
