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1.
Proc Natl Acad Sci U S A ; 102(9): 3301-6, 2005 Mar 01.
Article in English | MEDLINE | ID: mdl-15728366

ABSTRACT

The maintenance of photoreceptor cell polarity is compromised by the rhodopsin mutations causing the human disease autosomal dominant retinitis pigmentosa. The severe form mutations occur in the C-terminal sorting signal of rhodopsin, VXPX-COOH. Here, we report that this sorting motif binds specifically to the small GTPase ARF4, a member of the ARF family of membrane budding and protein sorting regulators. The effects of blocking ARF4 action were functionally equivalent to the effects of blocking the rhodopsin C-terminal sorting signal. ARF4 was essential for the generation of post-Golgi carriers targeted to the rod outer segments of retinal photoreceptors. Thus, the severe retinitis pigmentosa alleles that affect the rhodopsin sorting signal interfere with interactions between ARF4 and rhodopsin, leading to aberrant trafficking and initiation of retinal degeneration.


Subject(s)
ADP-Ribosylation Factors/metabolism , Mutation , Rhodopsin/physiology , Amino Acid Sequence , Animals , Blotting, Western , Electrophoresis, Polyacrylamide Gel , Golgi Apparatus/metabolism , Microscopy, Confocal , Protein Binding , Protein Transport , Ranidae , Reactive Oxygen Species , Rhodopsin/chemistry , Rhodopsin/genetics , Rhodopsin/metabolism
2.
J Mol Biol ; 343(5): 1439-50, 2004 Nov 05.
Article in English | MEDLINE | ID: mdl-15491622

ABSTRACT

Rhodopsin, the pigment protein responsible for dim-light vision, is a G protein-coupled receptor that converts light absorption into the activation of a G protein, transducin, to initiate the visual response. We have crystallised detergent-solubilised bovine rhodopsin in the native form and after chemical modifications as needles 10-40 microm in cross-section. The crystals belong to the trigonal space group P3(1), with two molecules of rhodopsin per asymmetric unit, related by a non-crystallographic 2-fold axis parallel with the crystallographic screw axis along c (needle axis). The unit cell dimensions are a=103.8 A, c=76.6 A for native rhodopsin, but vary over a wide range after heavy atom derivatisation, with a between 101.5 A and 113.9 A, and c between 76.6 A and 79.2 A. Rhodopsin molecules are packed with the bundle of transmembrane helices tilted from the c-axis by about 100 degrees . The two molecules in the asymmetric unit form contacts along the entire length of their transmembrane helices 5 in an antiparallel orientation, and they are stacked along the needle axis according to the 3-fold screw symmetry. Hence hydrophobic contacts are prominent at protein interfaces both along and normal to the needle axis. The best crystals of native rhodopsin in this crystal form diffracted X-rays from a microfocused synchrotron source to 2.55 A maximum resolution. We describe steps taken to extend the diffraction limit from about 10 A to 2.6 A.


Subject(s)
Metals, Heavy/chemistry , Rhodopsin/chemistry , Animals , Cattle , Crystallization , Crystallography, X-Ray , Detergents , Metals, Heavy/metabolism , Protein Structure, Tertiary , Retina/chemistry , Retina/metabolism , Rhodopsin/analogs & derivatives , Rhodopsin/isolation & purification , Rhodopsin/metabolism , Spectrophotometry
3.
J Biol Chem ; 279(49): 51203-7, 2004 Dec 03.
Article in English | MEDLINE | ID: mdl-15351781

ABSTRACT

Phosphorylation of activated G-protein-coupled receptors and the subsequent binding of arrestin mark major molecular events of homologous desensitization. In the visual system, interactions between arrestin and the phosphorylated rhodopsin are pivotal for proper termination of visual signals. By using high resolution proton nuclear magnetic resonance spectroscopy of the phosphorylated C terminus of rhodopsin, represented by a synthetic 7-phosphopolypeptide, we show that the arrestin-bound conformation is a well ordered helix-loop structure connected to rhodopsin via a flexible linker. In a model of the rhodopsin-arrestin complex, the phosphates point in the direction of arrestin and form a continuous negatively charged surface, which is stabilized by a number of positively charged lysine and arginine residues of arrestin. Opposite to the mostly extended structure of the unphosphorylated C-terminal domain of rhodopsin, the arrestin-bound C-terminal helix is a compact domain that occupies a central position between the cytoplasmic loops and occludes the key binding sites of transducin. In conjunction with other binding sites, the helix-loop structure provides a mechanism of shielding phosphates in the center of the rhodopsin-arrestin complex and appears critical in guiding arrestin for high affinity binding with rhodopsin.


Subject(s)
Arrestins/chemistry , Rhodopsin/chemistry , Animals , Arginine/chemistry , Binding Sites , Cattle , Cytoplasm/metabolism , Dose-Response Relationship, Drug , GTP-Binding Proteins/chemistry , Guanosine Triphosphate/chemistry , Lysine/chemistry , Magnetic Resonance Spectroscopy , Models, Molecular , Peptides/chemistry , Phosphorylation , Protein Binding , Protein Conformation , Protein Structure, Secondary , Protein Structure, Tertiary , Serine/chemistry , Signal Transduction
4.
FEBS Lett ; 564(3): 307-11, 2004 Apr 30.
Article in English | MEDLINE | ID: mdl-15111114

ABSTRACT

Visual arrestin binds to the phosphorylated carboxy-terminal region of rhodopsin to block interactions with transducin and terminate signaling in the rod photoreceptor cells. A synthetic seven-phospho-peptide from the C-terminal region of rhodopsin, Rh(330-348), has been shown to bind arrestin and mimic inhibition of signal transduction. In this study, we examine conformational changes in this synthetic peptide upon binding to arrestin by high-resolution proton nuclear magnetic resonance (NMR). We show that the peptide is completely disordered in solution, but becomes structured upon binding to arrestin. A control, unphosphorylated peptide that fails to bind to arrestin remains highly disordered. Specific NMR distance constraints are used to model the arrestin-bound conformation. The models suggest that the phosphorylated carboxy-terminal region of rhodopsin, Rh(330-348), undergoes significant conformational changes and becomes structured upon binding to arrestin.


Subject(s)
Arrestin/chemistry , Arrestin/metabolism , Protein Conformation , Rhodopsin/chemistry , Rhodopsin/metabolism , Animals , Cattle , Models, Molecular , Nuclear Magnetic Resonance, Biomolecular , Phosphorylation , Protein Binding , Signal Transduction
5.
Protein Sci ; 12(11): 2453-75, 2003 Nov.
Article in English | MEDLINE | ID: mdl-14573859

ABSTRACT

Rhodopsin is the best-understood member of the large G protein-coupled receptor (GPCR) superfamily. The G-protein amplification cascade is triggered by poorly understood light-induced conformational changes in rhodopsin that are homologous to changes caused by agonists in other GPCRs. We have applied the "antibody imprint" method to light-activated rhodopsin in native membranes by using nine monoclonal antibodies (mAbs) against aqueous faces of rhodopsin. Epitopes recognized by these mAbs were found by selection from random peptide libraries displayed on phage. A new computer algorithm, FINDMAP, was used to map the epitopes to discontinuous segments of rhodopsin that are distant in the primary sequence but are in close spatial proximity in the structure. The proximity of a segment of the N-terminal and the loop between helices VI and VIII found by FINDMAP is consistent with the X-ray structure of the dark-adapted rhodopsin. Epitopes to the cytoplasmic face segregated into two classes with different predicted spatial proximities of protein segments that correlate with different preferences of the antibodies for stabilizing the metarhodopsin I or metarhodopsin II conformations of light-excited rhodopsin. Epitopes of antibodies that stabilize metarhodopsin II indicate conformational changes from dark-adapted rhodopsin, including rearrangements of the C-terminal tail and altered exposure of the cytoplasmic end of helix VI, a portion of the C-3 loop, and helix VIII. As additional antibodies are subjected to antibody imprinting, this approach should provide increasingly detailed information on the conformation of light-excited rhodopsin and be applicable to structural studies of other challenging protein targets.


Subject(s)
Receptors, G-Protein-Coupled/chemistry , Rhodopsin/chemistry , Algorithms , Amino Acid Sequence , Amino Acid Substitution , Animals , Antibodies, Monoclonal/immunology , Cattle , Consensus Sequence , Crystallography, X-Ray , Cytoplasm/chemistry , Cytoplasm/metabolism , Darkness , Epitope Mapping/methods , Light , Models, Molecular , Molecular Sequence Data , Protein Binding , Protein Conformation , Receptors, G-Protein-Coupled/metabolism , Receptors, G-Protein-Coupled/radiation effects , Rhodopsin/immunology , Rhodopsin/radiation effects , Rod Cell Outer Segment/chemistry , Rod Cell Outer Segment/metabolism
6.
Exp Eye Res ; 76(5): 553-63, 2003 May.
Article in English | MEDLINE | ID: mdl-12697419

ABSTRACT

Subcellular translocation of phototransduction proteins in response to light has previously been detected by immunocytochemistry. This movement is consistent with the hypothesis that migration is part of a basic cellular mechanism regulating photoreceptor sensitivity. In order to monitor the putative migration of arrestin in response to light, we expressed a functional fusion between the signal transduction protein arrestin and green fluorescent protein (GFP) in rod photoreceptors of transgenic Xenopus laevis. In addition to confirming reports that arrestin is translocated, this alternative approach generated unique observations, raising new questions regarding the nature and time scale of migration. Confocal fluorescence microscopy was performed on fixed frozen retinal sections from tadpoles exposed to three different lighting conditions. A consistent pattern of localization emerged in each case. During early light exposure, arrestin-GFP levels diminished in the inner segments (ISs) and simultaneously increased in the outer segments (OSs), initially at the base and eventually at the distal tips as time progressed. Arrestin-GFP reached the distal tips of the photoreceptors by 45-75 min at which time the ratio of arrestin-GFP fluorescence in the OSs compared to the ISs was maximal. When dark-adaptation was initiated after 45 min of light exposure, arrestin-GFP rapidly re-localized to the ISs and axoneme within 30 min. Curiously, prolonged periods of light exposure also resulted in re-localization of arrestin-GFP. Between 150 and 240 min of light adaptation the arrestin-GFP in the ROS gradually declined until the pattern of arrestin-GFP localization was indistinguishable from that of dark-adapted photoreceptors. This distribution pattern was observed over a wide range of lighting intensity (25-2700 lux). Immunocytochemical analysis of arrestin in wild-type Xenopus retinas gave similar results.


Subject(s)
Adaptation, Ocular/physiology , Arrestin/metabolism , Retinal Rod Photoreceptor Cells/metabolism , Animals , Animals, Genetically Modified , Dark Adaptation/physiology , Green Fluorescent Proteins , Light , Luminescent Proteins , Microscopy, Confocal , Photic Stimulation , Recombinant Fusion Proteins/metabolism , Rod Cell Outer Segment/metabolism , Xenopus laevis
7.
J Clin Invest ; 111(8): 1171-80, 2003 Apr.
Article in English | MEDLINE | ID: mdl-12697736

ABSTRACT

Experimental autoimmune uveitis (EAU) is a disease of the neural retina induced by immunization with retinal antigens, such as interphotoreceptor retinoid-binding protein (IRBP) and arrestin (retinal soluble antigen, S-Ag). EAU serves as a model for human autoimmune uveitic diseases associated with major histocompatibility complex (HLA) genes, in which patients exhibit immunological responses to retinal antigens. Here we report the development of a humanized EAU model in HLA transgenic (TG) mice. HLA-DR3, -DR4, -DQ6, and -DQ8 TG mice were susceptible to IRBP-induced EAU. Importantly, HLA-DR3 TG mice developed severe EAU with S-Ag, to which wild-type mice are highly resistant. Lymphocyte proliferation was blocked by anti-HLA antibodies, confirming that antigen is functionally presented by the human MHC molecules. Disease could be transferred by immune cells with a Th1-like cytokine profile. Antigen-specific T cell repertoire, as manifested by responses to overlapping peptides derived from S-Ag or IRBP, differed from that of wild-type mice. Interestingly, DR3 TG mice, but not wild-type mice, recognized an immunodominant S-Ag epitope between residues 291 and 310 that overlaps with a region of S-Ag recognized by uveitis patients. Thus, EAU in HLA TG mice offers a new model of uveitis that should represent human disease more faithfully than currently existing models.


Subject(s)
Autoimmune Diseases/etiology , Eye Proteins , Histocompatibility Antigens Class II/physiology , Uveitis/etiology , Amino Acid Sequence , Animals , Antigen Presentation , Arrestin/immunology , Disease Models, Animal , Epitopes, T-Lymphocyte , HLA-DR3 Antigen/physiology , Humans , Mice , Mice, Inbred C57BL , Mice, Transgenic , Molecular Sequence Data , Retinol-Binding Proteins/immunology
8.
Cell Immunol ; 217(1-2): 87-94, 2002.
Article in English | MEDLINE | ID: mdl-12426004

ABSTRACT

Lewis rats immunized with myelin basic protein (MBP) developed experimental autoimmune encephalomyelitis (EAE) and associated anterior uveitis (AU). Although several cryptic epitopes of MBP have strong encephalitogenic and uveitogenic properties, the peptide corresponding to the MBP residues 1-20 was uniquely capable of inducing AU without EAE. In this study, we showed that acetylation of the N-terminal amino acid did not produce encephalitogenicity, did not enhance uveitogenicity, and did not improve T cell proliferation in Lewis rats. The cytokine production profile induced by MBP(1-20) immunization was consistent with a Th1 response. In MBP-injected rats and in peptide-injected rats, the frequency of the IFN-gamma-secreting cells in MBP(69-89)-stimulated T cells was significantly higher than the frequency of IFN-gamma-secreting cells in MBP(1-20)-stimulated T cells. However, similar numbers of IFN-gamma-producing specific cells were found in the eyes of MBP(69-89) and MBP(1-20) immunized rats. In these rats, the iris-infiltrating cells consisted of a much higher percentage of CD4(+) T cells expressing L-selectin (CD62L) than did those cells found in the spinal cord. The results demonstrate that MBP(1-20) is immunogenic and uveitogenic, although it induced only weak proliferation and weak Th1 reaction. The fact that T cells with the same specificity have different effects on target organs suggested that, in the eye and spinal cord, a distinct mechanism might mediate the recruitment of cells to these organs.


Subject(s)
Autoimmune Diseases/immunology , Encephalomyelitis, Autoimmune, Experimental/immunology , Epitopes/immunology , Myelin Basic Protein/immunology , Peptide Fragments/immunology , Uveitis/immunology , Acetylation , Amino Acid Sequence , Animals , Autoimmune Diseases/pathology , CD4-Positive T-Lymphocytes/immunology , Cells, Cultured , Cytokines/biosynthesis , Female , L-Selectin/metabolism , Lymphocyte Activation , Molecular Sequence Data , Myelin Basic Protein/chemistry , Peptide Fragments/chemistry , Rats , Rats, Inbred Lew , Uveitis/pathology
9.
Eur J Biochem ; 269(15): 3801-9, 2002 Aug.
Article in English | MEDLINE | ID: mdl-12153577

ABSTRACT

Visual arrestin is converted from a 'basal' state to an 'activated' state by interaction with the phosphorylated C-terminus of photoactivated rhodopsin (R*), but the conformational changes in arrestin that lead to activation are unknown. Small-angle X-ray scattering (SAXS) was used to investigate the solution structure of arrestin and characterize changes attendant upon activation. Wild-type arrestin forms dimers with a dissociation constant of 60 micro m. Small conformational changes, consistent with local movements of loops or the mobile N- or C-termini of arrestin, were observed in the presence of a phosphopeptide corresponding to the C-terminus of rhodopsin, and with an R175Q mutant. Because both the phosphopeptide and the R175Q mutation promote binding to unphosphorylated R*, we conclude that arrestin is activated by subtle conformational changes. Most of the arrestin will be in a dimeric state in vivo. Using the arrestin structure as a guide [Hirsch, J.A., Schubert, C., Gurevich, V.V. & Sigler, P.B. (1999) Cell 97, 257-269], we have identified a model for the arrestin dimer that is consistent with our SAXS data. In this model, dimerization is mediated by the C-terminal domain of arrestin, leaving the N-terminal domains free for interaction with phosphorylated R*.


Subject(s)
Arrestin/chemistry , Amino Acid Sequence , Arrestin/genetics , Arrestin/metabolism , Dimerization , Models, Molecular , Molecular Sequence Data , Mutation , Peptide Fragments/chemistry , Peptide Fragments/metabolism , Phosphoproteins/chemistry , Phosphoproteins/metabolism , Phosphorylation , Protein Conformation , Rhodopsin/chemistry , Rhodopsin/genetics , Rhodopsin/metabolism , Scattering, Radiation , Solutions , X-Rays
11.
J Biol Chem ; 277(14): 11703-8, 2002 Apr 05.
Article in English | MEDLINE | ID: mdl-11809770

ABSTRACT

Visual arrestin inactivates the phototransduction cascade by specifically binding to light-activated phosphorylated rhodopsin. This study describes the combined use of insertional mutagenesis and immunochemical approaches to probe the structural determinants of arrestin function. Recombinant arrestins with insertions of a 10-amino acid c-Myc tag (EQKLISEEDL) were expressed in yeast and characterized. When the tag was placed on the C terminus after amino acid 399, between amino acids 99 and 100 or between residues 162 and 163, binding to rhodopsin was found to be very similar to that of wild-type arrestin. Two stable mutants with Myc insertions in the 68-78 loop were also generated. Binding to rhodopsin was markedly decreased for one (72myc73) and completely abolished for the other (77myc78). Limited proteolysis assays using trypsin in the absence or presence of heparin were performed on all mutants and confirmed their overall conformational integrity. Rhodopsin binding to either 162myc163 or 72myc73 arrestins in solution was completely inhibited in the presence of less than a 2-fold molar excess of anti-Myc antibody relative to arrestin. In contrast, the antibody did not block the interaction of the 399myc or 99myc100 arrestins with rhodopsin. These results indicate that an interactive surface for rhodopsin is located on or near the concave region of the N-domain of arrestin.


Subject(s)
Arrestin/metabolism , Mutagenesis , Rhodopsin/metabolism , Amino Acid Sequence , Animals , Arrestin/genetics , Binding, Competitive , Cattle , Dose-Response Relationship, Drug , Epitopes , Immunohistochemistry , Light , Models, Molecular , Molecular Sequence Data , Mutation , Pichia/metabolism , Protein Binding , Protein Conformation , Protein Structure, Tertiary , Recombinant Proteins/metabolism , Rhodopsin/chemistry , Rhodopsin/genetics , Trypsin/pharmacology
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