Effect of intrauterine administration of gonadotropin releasing hormone with glycerol on serum LH concentrations in lactating dairy cows.

The objectives of the study were to assess: (1) preovulatory serum LH concentrations and (2) synchrony of ovulation after im or iu administration of GnRH with or without the addition of glycerol. Cows were presynchronized with 2 injections of PGF2α given 14d apart (starting at 26±3DIM) followed by Ovsynch (OV; GnRH-7d-PGF2α-48h-GnRH) 12d later. At the time of the second GnRH of OV (GnRH2), cows were blocked by parity and randomly allocated to 1 of 4 treatments: (1) control (CON; n=8) received 2mL of sterile water im; (2) im (IM; n=8) received 100µg of GnRH im; (3) cows were infused with 200µg GnRH into the uterus (IU; n=9); and (4) iu administration of 200µg GnRH plus glycerol 7% v/v (IUG; n=8). Serum circulating progesterone concentrations at hour 0 did not differ (P>0.05) among groups. Concentrations of LH were greater (P<0.05) in IM than IU, IUG, and CON cows at hours 1, 1.5, 2, and 3. All cows ovulated within 48h in the IM (8/8) group followed by IU (6/9) and IUG (4/8) groups, and only two out of eight cows ovulated in the CON group. Although iu administration of GnRH in the IU and IUG groups resulted in lower serum concentrations of LH than IM cows, IU or IUG cows were able to ovulate within 48h after GnRH2 administration.

Isotopic investigation of the colloidal mobility of depleted uranium in a podzolic soil.

The mobility and colloidal migration of uranium were investigated in a soil where limited amounts of anthropogenic uranium (depleted in the 235U isotope) were deposited, adding to the naturally occurring uranium. The colloidal fraction was assumed to correspond to the operational fraction between 10 kDa and 1.2 µm after (ultra)filtration. Experimental leaching tests indicate that approximately 8-15% of uranium is desorbed from the soil. Significant enrichment of the leachate in the depleted uranium (DU) content indicates that uranium from recent anthropogenic DU deposit is weakly bound to soil aggregates and more mobile than geologically occurring natural uranium (NU). Moreover, 80% of uranium in leachates was located in the colloidal fractions. Nevertheless, the percentage of DU in the colloidal and dissolved fractions suggests that NU is mainly associated with the non-mobile coarser fractions of the soil. A field investigation revealed that the calculated percentages of DU in soil and groundwater samples result in the enhanced mobility of uranium downstream from the deposit area. Colloidal uranium represents between 10% and 32% of uranium in surface water and between 68% and 90% of uranium in groundwater where physicochemical parameters are similar to those of the leachates. Finally, as observed in batch leaching tests, the colloidal fractions of groundwater contain slightly less DU than the dissolved fraction, indicating that DU is primarily associated with macromolecules in dissolved fraction.