Rickettsia parkeri and "Candidatus Rickettsia andeanae" in Questing Amblyomma maculatum (Acari: Ixodidae) From Mississippi.

Amblyomma maculatum Koch (Acari: Ixodidae), the primary vector for Rickettsia parkeri, may also be infected with a rickettsia of unknown pathogenicity, "Candidatus Rickettsia andeanae." Infection rates with these rickettsiae vary geographically, and coinfected ticks have been reported. In this study, infection rates of R. parkeri and "Ca. R. andeanae" were evaluated, and rickettsial DNA levels quantified, in 335 questing adult A. maculatum collected in 2013 (n = 95), 2014 (n = 139), and 2015 (n = 101) from Oktibbeha County, MS. Overall infection rates of R. parkeri and "Ca. R. andeanae" were 28.7% and 9.3%, respectively, with three additional A. maculatum (0.9%) coinfected. While R. parkeri-infected ticks were detected all three years (34.7% in 2013; 13.7% in 2014; 43.6% in 2015), "Ca. R. andeanae" was not detected in 2013, and was detected at rates of 10.8% in 2014, and 15.8% in 2015. Interestingly, rickettsial DNA levels in singly-infected ticks were significantly lower in "Ca. R. andeanae"-infected ticks compared to R. parkeri-infected ticks (P < 0.0001). Thus, both infection rates and rickettsial DNA levels were higher for R. parkeri than "Ca. R. andeanae." Infection rates of R. parkeri were also higher, and "Ca. R. andeanae" lower, here compared to A. maculatum reported previously in Kansas and Oklahoma. As we continue to monitor infection rates and levels, we anticipate that understanding temporal changes will improve our awareness of human risk for spotted fever rickettsioses. Further, these data may lead to additional studies to evaluate potential interactions among sympatric Rickettsia species in A. maculatum at the population level.

Evolutionary Stability for Interactions among Kin under Quantitative Inheritance.

The theory of evolutionarily stable strategies (ESS) predicts the long-term evolutionary outcome of frequency-dependent selection by making a number of simplifying assumptions about the genetic basis of inheritance. I use a symmetrized multilocus model of quantitative inheritance without mutation to analyze the results of interactions between pairs of related individuals and compare the equilibria to those found by ESS analysis. It is assumed that the fitness changes due to interactions can be approximated by the exponential of a quadratic surface. The major results are the following. (1) The evolutionarily stable phenotypes found by ESS analysis are always equilibria of the model studied here. (2) When relatives interact, one of the two conditions for stability of equilibria differs between the two models; this can be accounted for by positing that the inclusive fitness function for quantitative characters is slightly different from the inclusive fitness function for characters determined by a single locus. (3) The inclusion of environmental variance will in general change the equilibrium phenotype, but the equilibria of ESS analysis are changed to the same extent by environmental variance. (4) A class of genetically polymorphic equilibria occur, which in the present model are always unstable. These results expand the range of conditions under which one can validly predict the evolution of pairwise interactions using ESS analysis.

Bone mineral and vitamin D in Aleutian Islanders.

Serum concentrations of vitamin D metabolites (chromatography) and bone mineral status (125I absorptiometry) were examined in a group of Aleutian Islanders age 40-75 from St Paul Island, Alaska. Based on 25-(OH)D (16.6 ng/ml) vitamin D status appeared adequate. However, high concentrations of 1,25-(OH)2D (44.3 pg/ml) and very low concentrations of 24,25-(OH)2D3 (0.6 ng/ml) were found. Among females, low bone mineral levels were associated with high concentrations of 1,25-(OH)2D. A low calcium intake in these Aleutians may be responsible for high concentrations of 1,25-(OH)2D and resorption of calcium from bone.

Physical growth of St. Lawrence Island Eskimos: body size, proportion, and composition.

Growth patterns of body size, proportion, and composition were analyzed in 57 male and 56 female Eskimos from St. Lawrence Island in the Bering Sea, ranging in age from 1.23 through 19.82 years. Age-groups means for whites and blacks of the U.S. Health Examination Survey served as reference data. Relative to HES data, the Eskimo sample were shorter with lower values for leg length, while there were no differences from the reference values for sitting height. The Eskimos also had higher values of Quetelet's Index, the sitting height/height ratio, and the upper arm muscle circumference, while there were no differences in body weight or triceps skinfold thickness. Differences from the reference data were more pronounced in males than in females. The growth patterns for size and body proportion are in conformity with known relationships between morphology and climate.

Origins and divergence of Aleuts, Eskimos, and American Indians.

The apportionment of average gene frequency differences into within and between groups of Aleuts, Eskimos and Athabascans reveals a testable model of the time of origin and differentiation of these populations. Based on the ratio of average difference between Aleuts and Eskimos, to the average difference between Bering Sea Mongoloids and Athabascans, we estimate that Athabascans diverged from Bering Sea Mongoloids at approximately 15 000 BP. The ratio of Aleut/Eskimo to Yupik/Inupiaq suggests the split between the latter occurred 5100 BP. Similarly, the within-group average gene frequency differences suggest that North American natives originated some 19 000 BP and that Bering Sea Mongoloids originated 10 200 BP. These estimates are highly concordant with independent archaeologic, linguistic and biological data.