The Werner syndrome protein is required for recruitment of chromatin assembly factor 1 following DNA damage.

The Werner syndrome protein (WRN) and chromatin assembly factor 1 (CAF-1) are both involved in the maintenance of genome stability. In response to DNA-damaging signals, both of these proteins relocate to sites where DNA synthesis occurs. However, the interaction between WRN and CAF-1 has not yet been investigated. In this report, we show that WRN interacts physically with the largest subunit of CAF-1, hp150, in vitro and in vivo. Although hp150 does not alter WRN catalytic activities in vitro, and the chromatin assembly activity of CAF-1 is not affected in the absence of WRN in vivo, this interaction may have an important role during the cellular response to DNA replication fork blockage and/or DNA damage signals. In hp150 RNA-mediated interference (RNAi) knockdown cells, WRN partially formed foci following hydroxyurea (HU) treatment. However, in the absence of WRN, hp150 did not relocate to form foci following exposure to HU and ultraviolet light. Thus, our results demonstrate that WRN responds to DNA damage before CAF-1 and suggest that WRN may recruit CAF-1, via interaction with hp150, to DNA damage sites during DNA synthesis.

Human diseases deficient in RecQ helicases.

RecQ helicases are conserved from bacteria to man. Mutations in three of the human RecQ family members give rise to genetic disorders characterized by genomic instability and a predisposition to cancer. RecQ helicases are therefore caretakers of the genome, and although they do not directly regulate tumorigenesis, they influence stability and the rate of accumulation of genetic alterations, which in turn, result in tumorigenesis. Maintenance of genome stability by RecQ helicases likely involves their participation in DNA replication, recombination, and repair pathways.

Detecting anxiety and defensiveness from visual and auditory cues.

Defensive individuals have been shown to differ from nondefensive individuals on a number of physiological and behavioral measures. We report two studies on observers' inferences of defensiveness, and the contribution of communication channels in the inference of defensiveness. Observers judged high and low state anxious segments of high and low trait anxious defensive and nondefensive individuals. Accurate assessments were made of (a) defensiveness, (b) state anxiety, and (c) trait anxiety: Individuals with higher levels of each variable were perceived as more anxious compared with the lower level. Effects for defensiveness and state anxiety were greater in audio-only segments, while effects for trait anxiety were greater in video-only segments. Inferences of defensiveness were greater at higher levels of state anxiety and trait anxiety. Low trait anxious defensive individuals were perceived as more anxious than the true low trait anxious. Results for defensiveness and trait anxiety were replicated in Study 2, and observers' perceptions of state anxiety matched individuals' self-reports: Defensive individuals with maximal differences between high and low state anxiety were seen as more anxious in high state anxiety, while defensive individuals with minimal differences between high and low state anxiety were regarded as less anxious in high state anxiety.

Disclosure and anxiety: a test of the fever model.

According to the fever model of Disclosure, the relationship of Disclosure (utterances that reveal subjective information) to psychological distress is analogous to the relationship of fever to physical infection: Both are indicators of some underlying disturbance and part of a restorative process. High and moderate trait anxious university students (but not the low trait anxious students) used higher percentages of Disclosure when speaking about an anxiety-arousing topic than when speaking about a happy topic. With topic order counterbalanced, students tended to use more Disclosure during their 1st presentation than during their 2nd. These results support the fever model's suggestion that people tend to Disclose when they are distressed.

Analysis of verbal behavior between physicians and geriatric patients.

This study was designed to analyze communication patterns between older patients and physicians by an in-depth examination of linguistic aspects of the interactions, including utterance form, process, and content variables. Sixteen family medicine residents were videotaped individually interviewing one of four simulated female patients. Verbal behavior was analyzed in detail using categories from linguistic studies. Residents were divided into High and Low Information groups. Significant differences in interviewing style were found between groups in categories of: Closed (High = 62.25, Low = 35.88), Compound (High = 11.63, Low = 2.00), and Reflective (High = 20.13, Low = 10.13) Questions; Direct (High = 33.50, Low = 25.00) and Positive (High = 9.25, Low = 3.88) Answers; Added Information (High = 15.75; Low = 9.38); Listener Responses (High = 57.38, Low = 41.50); and Appropriate Turn-Taking (High = 66.63, Low = 48.38). There seems to be a clear difference between what is taught in medical schools about interviewing and the interviewing techniques used by residents in the actual practice of medicine. High information doctors combined a style of closed and combined questions with considerable facilitation and respect for the patient.

It's how you say it: physicians' vocal behavior.

The present study was designed to assess evaluations of physicians interacting with patients via the telephone. Observers used ten adjective scales which resulted in three variables: empathic, dominant, and calm. Thirty doctor-patient interactions were presented in two different communication modes: audio-only and typed transcript-only. As predicted, female listeners rated doctors as more empathic, dominant, and calm, and communication modes were significantly different with audio segments rated as more empathic, dominant and calm. Middle phases of the conversation also were evaluated more positively than greeting phases. Significant interactions between temporal phase and mode indicated that audio segments were interpreted more positively during middle phases. Also, female listeners were more sensitive to audio segments. Physicians' amplitude and speech rate were positively correlated with dominance.

Nonverbal expressions of anxiety in physician-patient interactions.

NONVERBAL expressions of anxiety were analyzed during patient presentation of conflicted emotional topics (i.e., hidden agendas) while consulting with family medicine practitioners. It was hypothesized that underlying anxiety would be revealed in higher frequencies of specific types of nonverbal behavior. As predicted, hand-to-body self-touching occurred significantly more often during presentation of anxiety-producing topics, while frequencies of speech-illustrative gestures did not differ for type of agenda presented. Applications for these findings are discussed with reference to medical and psychotherapy encounters.

Children of alcoholics.

Parental alcoholism affects 27 million children, but few of these children receive supportive care. The most consistent predictor of alcoholism is alcoholism in a biologic parent. A child's self-concept is derived largely from experiences at home and identification with the parents. Family physicians are in a unique position to recognize parental alcoholism, provide empathic concern for the children and direct them to supportive programs.

The telephone management of gastroenteritis by family medicine residents.

In order to describe the telephone management of gastroenteritis by family practice residents, audiotapes and transcripts of telephone calls by 31 family practice residents were analysed with respect to clinical content, temporal patterns, and physicians' utterance form. The study hypothesis were that year 3 residents would have shorter calls, take more complete histories, and score better on other performance measures than year 1 residents, but no significant differences were demonstrated in the study. The mean duration of telephone calls was 4.6 minutes. Residents talked three times longer than the caller. History taking completeness was highly correlated with telephone call duration and particularly with time spent talking by the caller. Significant questions, such as asking about hydration status, were frequently omitted. Most questions were closed (84 percent). Time spent talking by the caller was longer, and more listener feedback was given, with year 1 than with year 3 residents.

Does the family physician's role correspond to the patient's expectations?

A telephone survey of Ohio residents was undertaken to measure how respondents rate 12 selected characteristics of physicians and medical practices when choosing a doctor. All parameters were found to be important, some more than others. "The doctor being able to explain things in an easily understood way" ranked first, followed closely by "being able to reach the doctor easily for emergencies," and "the doctor's fees being reasonable." The effect of several demographic characteristics of the 12 parameters was tested; only age of the respondent had a consistent impact. Factor analysis showed that the interests of potential patients centered primarily in the areas of interpersonal relations, continuity of care, access to services, and activities such as preventive care, all of which are highly valued by family medicine.

Self-touching as an indicator of underlying affect and language processes.

In this study of 28 physicians and their patients during the medical interview, information is presented on a class of nonverbal behavior, self-touching, thought to represent states of negative affect. A more recent perspective suggests that self-touching is associated with cognitive processing. The results of the present study focused on differences in types of self-touching by patients and physicians, semantic content of utterances when self-touching was displayed, and temporal location of self-touching within the speech stream. These are considered with respect to affective and psycholinguistic theories of self-touching. The results lend support to the notion that self-touching is exhibited in relation to information processing and production, in addition to negative affect.

Inferences of mental illness from noninvolvement.

These studies continue the exploration of variables related to a person's use of the mental illness categorization. The central concern in the present studies was the effect of perceived variation in a target person's level of involvement in a social situation. While a low level of involvement, as portrayed in videotaped scenarios, prompts attribution of mental illness, other features of implicit personality theories also relate to greater or lesser attribution of mental illness. Those participants who gave evidence of having attributed lower levels of involvement, regardless of filmed information, also attributed higher levels of mental illness. Social workers, compared to general population participants, attributed higher levels of mental illness at all levels of target involvement. We discuss the implications of these findings for dissemination and assignment of the mentally ill role.