The role of species and geography in the elemental profiles of farm-raised shrimp from Indonesia.

Elemental profiling is being explored as a traceability tool in many seafood products. However, the extent that elemental profiling can be used at finer geographical scales in cultured shrimp is unknown. Additionally, few studies have included multiple species in the same discriminant models, which would be useful in applications where one species is common, and the other is not. Here, elemental profiling was used to discern the provenance of black tiger shrimp Penaeus monodon and whiteleg shrimp Litopenaeus vannamei from the regions of North Kalimantan, Sulawesi Seletan, and Aceh in Indonesia. ICP-MS was used to determine elemental concentrations of 41 elements in shrimp muscle tissue and was the basis for multivariate and univariate statistical analyses. A MANOVA showed that multivariate differences exist in regions and between species of shrimp sampled. Univariate comparisons were utilized after the significance of the MANOVA and showed that 19 of the 24 elements above detection limits had significant differences. Classification via random forest was used to access the ability to discern, region, species, and region × species group combinations. The lowest model accuracy was the region × species combinations at 78.9%, while the highest accuracy was species irrespective of geographical origin at 93.59%. Elements that were routinely important in classification included As, B, Ba, Li, Na, Rb, Se, and Zn. Elemental profiles of white leg shrimp and black tiger shrimp are varied and potentially should not be used in the same classification models. Altogether, these results suggest that elemental profiling of farmed shrimp at finer geographic scales needs refinement as a traceability tool.

Sources of variation in elemental profiles of whiteleg shrimp (Litopenaeus vannamei) and their potential effects on the accuracy of discriminant analysis.

BACKGROUND: Elemental profiling is a tool that has been proposed to improve the traceability of seafood products. Small sources of variation can affect the outcome of elemental profiling and therefore pose to lower the overall accuracy of analyses. Here, we investigate two potential sources of variation through three experiments: laboratory variation (intra-, interlaboratory variation, and tissue matrix) and tissue variation. METHODS AND RESULTS: Samples of whitleleg shrimp (Litopenaeus vannamei) were obtained from 20 farms in Ecuador and two farms in Alabama to be analyzed. In the first experiment of the study, samples from Ecuador were analyzed at three different laboratories and compared. Two out of the five elements reported were statistically different across the three laboratories (Cu and Se). In the second experiment, the effect of tissue matrix (ground vs whole tissue during acid digestion) was investigated. Altogether, five out of 29 elements analyzed were statistically different. In the third experiment, samples from two farms in Alabama were analyzed to understand the variation in element concentrations in different tissues (head on shell on (HOSO), headless shell on (HLSO), headless peeled (PLD) and headless peeled and deveined (PLDV)). Elemental concentrations varied across tissues, and patterns in elemental concentrations were site specific. The samples from the two farms were analyzed with a Random Forest classification model to site x tissue groupings with 94% accuracy. CONCLUSION: The result of this study highlights the following: 1. Consistency in laboratory analysis important in studies that involve element concentrations, as minor differences in methodology can propagate as significant differences in results. 2. In shrimp, elements are compartmentalized in different tissues and elemental profiling should consistently use the same type of tissue.

Trace element concentrations in white leg shrimp Litopenaeus vannamei from retail stores in the EU, UK, and USA and the ability to discern country of origin with classification models.

Shrimp are a globally traded aquaculture commodity that accounts for a large proportion of the monetary value of aquaculture. There are concerns among consumers about seafood labeling fraud and environmental sustainability. Therefore, the geographic origin of shrimp from retail stores was investigated with trace element profiling. 94 shrimp samples were collected from grocery stores across the USA, UK, and EU in 70 different grocery stores. The results of 24 elements are reported. Shrimp samples were from Thailand, India, Vietnam, Indonesia, and Ecuador were shown to have 15 elements that were statistically different across labeled country of origin, with Ecuador having unique post hoc group membership in 5 of the elements. Based on a classification procedure, shrimp were classified to labeled country of origin with an overall accuracy of 71.2%. Overall, the results suggest that elemental profiling could be a traceability tool for classifying samples of shrimp from retail stores.

Usefulness of low-dose combined spinal epidural anesthesia for a woman with systemic lupus erythematosus, mechanical prosthesis in the mitral valve position, and worsening heart failure presenting for cesarean delivery.

A 31-year-old woman, gravida one, para zero, at 32 weeks, 4 days gestation, with a history of antiphospholipid antibody syndrome, mitral valve replacement requiring anticoagulation, chronic diastolic heart failure, and systemic lupus erythematous was admitted to the hospital for worsening cardiac decompensation with superimposed pneumonia. She was on warfarin for anticoagulation at the time of hospital admission and eventually started on an intravenous heparin infusion. Cesarean delivery was planned due to comorbidities and anticoagulation status. After administration of betamethasone for fetal lung maturity, the patient's heparin infusion was discontinued approximately 16 hours prior to cesarean delivery. Upon obtaining laboratory testing that confirmed appropriate coagulation status, a low-dose combined spinal epidural anesthetic technique was used for cesarean delivery and the expected hemodynamic shifts due to spinal anesthesia were mitigated with a prophylactic norepinephrine infusion.

Comparison of clinical and patient-reported outcomes with bilateral AcrySof toric or spherical control intraocular lenses.

PURPOSE: To compare clinical and patient-reported outcomes with bilateral implantation of AcrySof toric or spherical control (Alcon Laboratories Inc) intraocular lenses (IOLs). METHODS: Patients with cataract and corneal astigmatism who previously received either an AcrySof toric IOL or an AcrySof spherical control IOL were offered implantation of the same IOL in the fellow eye. Six-month assessments included visual acuity, refractive cylinder, spectacle use, and patient satisfaction. RESULTS: The study included 62 patients (toric, n=40; control, n=22). All corneal incisions were temporal, with final mean incision sizes of 3.0 mm for the toric IOL and 3.1 mm for the spherical control IOL. A significantly greater proportion of patients with toric IOLs achieved spectacle independence for distance vision and did not require prescription glasses for near or distance vision (P=.0190). Patients with toric IOLs had significantly less residual refractive cylinder (P<.0001) and better binocular distance uncorrected visual acuity (UCVA) (P=.0014) than those with spherical control IOLs. CONCLUSIONS: Patients with bilateral AcrySof toric IOLs achieved superior spectacle freedom, residual refractive cylinder, and distance UCVA compared to patients with bilateral spherical control IOLs.

Quality-of-life improvements in cataract patients with bilateral blue light-filtering intraocular lenses: clinical trial.

PURPOSE: To compare change in patient-reported vision-related and health-related functioning and quality of life (HRQOL) following bilateral implantation with a new blue light-filtering intraocular lens (IOL) with the results of a similar IOL that does not filter blue light. SETTING: Six clinical sites in the United States. METHODS: Patients were from 6 clinical sites in the United States that performed a high volume of cataract surgeries. The HRQOL assessments occurred via telephone while patients were at home. Patients requiring bilateral cataract extraction were randomly assigned to 1 of the 2 IOL groups for the first eye. The second eye was later implanted with the same type of IOL. Patients and HRQOL data collectors were treatment-masked, but investigators could not be. Health-related functioning and quality of life was measured with the 39-item National Eye Institute Visual Functioning Questionnaire (NEI VFQ-39) and 12-item Short Form Health Survey (SF-12). Assessments were at baseline before implantation in the first eye and 30 to 60 days and 120 to 180 days after implantation of the lens in the second eye. RESULTS: Both IOL types improved most aspects of patients' HRQOL including color vision and driving. The largest gains occurred on the VFQ composite, general vision, near activities, distance activities, driving, mental health, peripheral vision, and role difficulties scales. Significant gains also occurred on color vision and other vision-specific scales as well as the SF-12 physical component summary score. There were no significant differences in HRQOL gains between the IOLs. CONCLUSION: The blue light-filtering IOL improved color vision, driving, and other aspects of HRQOL in a manner similar to that of a lens that does not filter blue light.