ABSTRACT
The murine toxins A and B of Y. pestis were purified from a crude extract of the nonencapsulated strain M23NP of Y. pestis using HPLC gel filtration. Toxins A and B had molecular weights of 240,000 and 120,000, respectively. Both toxins had isoelectric points of 3.8. The mouse LD50 of toxin A was 2.5 micrograms, that of toxin B was 1.8 micrograms. Of the protein injected on the column, approximately 9% was toxin, including 2.9% toxin A and 6.1% toxin B. The crude M23NP extract was used periodically over 10 months to purify lots of the toxins. Each HPLC purification required about 30 min.
Subject(s)
Bacterial Toxins/isolation & purification , Yersinia pestis , Animals , Antibodies, Monoclonal , Bacterial Toxins/toxicity , Chromatography, High Pressure Liquid , Lethal Dose 50 , Mice , Plague/diagnosisABSTRACT
One hundred isolates of Yersinia pestis identified by conventional means were tested by the Micro-ID system to assess its reliability for distinguishing Y. pestis from other members of the family Enterobacteriaceae. The Micro-ID system gave Y. pestis as a choice for the identification of 89 of these cultures, although not always as the first choice. Most nitrate-negative strains of Y. pestis keyed out with Yersinia pseudotuberculosis as first choice and Y. pestis as second or fourth choice.
Subject(s)
Yersinia pestis/classification , Humans , MethodsSubject(s)
Bacteriocins/pharmacology , Yersinia/pathogenicity , Animals , Mice , Yersinia/classification , Yersinia/drug effectsSubject(s)
Rodentia/microbiology , Siphonaptera/microbiology , Yersinia pestis/isolation & purification , Animals , Humans , Indonesia , Microbial Sensitivity Tests , Rats , Sciuridae , Streptomycin/pharmacology , Tetracycline/pharmacology , United States , Vietnam , Virulence , Yersinia pestis/drug effectsSubject(s)
Antibodies, Bacterial/analysis , Disease Vectors , Yersinia pestis/immunology , Animals , Animals, Newborn , Bacteriolysis , Bacteriophages , Female , Hemagglutination Tests , Immunity, Maternally-Acquired , Maternal-Fetal Exchange , Milk/immunology , Pregnancy , Rats , Spleen/microbiology , Yersinia pestis/isolation & purificationSubject(s)
Animals, Domestic , Plague/epidemiology , Yersinia pestis/immunology , Animals , Antibody Formation , Antibody Specificity , Antigens, Bacterial , Complement Fixation Tests , Complement System Proteins , Guinea Pigs/immunology , Hemagglutination Tests , Mouth/microbiology , Plague/immunology , Plague/microbiology , Swine , Yersinia pestis/isolation & purification , ZoonosesSubject(s)
Plague/veterinary , Rodent Diseases/etiology , Animals , Injections, Intraperitoneal , Injections, Subcutaneous , Mice , Plague/etiology , Plague/immunology , Plague/pathology , Plague Vaccine/administration & dosage , Rats , Rodent Diseases/immunology , Rodent Diseases/pathology , Rodentia , Yersinia pestisSubject(s)
Bacterial Vaccines/administration & dosage , Pasteurella Infections/immunology , Administration, Oral , Animals , Antibody Formation , Complement Fixation Tests , Haplorhini , Hemagglutination Tests , Immunization , Immunization, Secondary , Infusions, Parenteral , Injections, Intramuscular , Lethal Dose 50 , Macaca , Yersinia pestis/immunologyABSTRACT
A pseudolysogenic strain of Pasteurella pestis was isolated from a human in Vietnam. The culture contained a stable mixture of bacteriophage and phage-sensitive and phage-resistant P. pestis.
