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Carbohydr Res
; 339(11): 1857-71, 2004 Aug 02.
Article
in English
| MEDLINE
| ID: mdl-15261579
ABSTRACT
The products from the enzymatic beta-D-galactopyranosylation of 1D-chiro-inositol, 1D-pinitol, 1D-3-O-allyl-4-O-methyl-chiro-inositol, 1D-3,4-di-O-methyl-chiro-inositol, 1L-chiro-inositol and myo-inositol in combined yields ranging from 46% to 64% have been obtained using the beta-galactosidase isolated from an anaerobic extreme thermophile, Thermoanaerobacter sp. strain TP6-B1 and p-nitrophenyl beta-D-galactopyranoside as the donor. Analysis of the products from these reactions reveals information about the acceptor preferences of the enzyme.
Subject(s)
Galactose/chemistry , Inositol/analogs & derivatives , Inositol/chemical synthesis , Thermoanaerobacter/enzymology , beta-Galactosidase/chemistry , Carbohydrate Conformation , Carbohydrate Sequence , Catalysis , Galactose/analogs & derivatives , Glycosylation , Methylation , Molecular Sequence Data , Stereoisomerism , beta-Galactosidase/isolation & purification
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