ABSTRACT
A green microalgae strain, Chlorococcum sp. was obtained from tropical freshwater in Indonesia. The effects of pH on growth, effects of salt, carbon dioxide level, nitrate, acetate, and photoperiod on biomass and lipid production were studied. The fatty acids composition was also investigated. This study shows that the strain had an optimum pH value for growth ranging from pH 8.0 to 8.5. The biomass concentration and lipid content were influenced by different concentrations of salt, CO(2) and nitrate. The lipid productivity ranged from 2 to 90.8 mg L(-1)d(-1) in different mediums. The highest biomass concentration and total lipid content achieved were 1.75 g L(-1) and 56% of dry weight, respectively. Moreover, the major fatty acid methyl esters were C16:0, C18:1, C18:2 and C18:3. The high lipid content and the fatty acid composition make the strain Chlorococcum sp. a potential resource for food, cosmetics and biodiesel.
Subject(s)
Biomass , Biotechnology/methods , Chlorophyta/metabolism , Fresh Water/microbiology , Lipids/biosynthesis , Microalgae/metabolism , Acetates/analysis , Carbon Dioxide/analysis , Chlorophyta/growth & development , Chromatography, Gas , Chromatography, High Pressure Liquid , Hydrogen-Ion Concentration , Indonesia , Microalgae/growth & development , Nitrates/analysis , Photoperiod , Spectrophotometry , Tropical ClimateABSTRACT
An aerobic, Gram-negative, motile bacterium, strain C02(T), was isolated from seawater obtained from Semarang Port in Indonesia. Cells of strain C02(T) were peritrichously flagellated and rod-shaped. Strain C02(T) was able to degrade naphthalene, alkylnaphthalenes and phenanthrene. 16S rRNA gene sequence analysis revealed that this strain was affiliated with the family Rhodobacteraceae in the class Alphaproteobacteria and was related most closely to Marinovum algicola FF3(T) (95.7 % similarity) and Thalassobius aestuarii JC2049(T) (95.2 %). The DNA G+C content of strain C02(T) was 64.6 mol%. The major cellular fatty acids were C(18 : 1)omega7c (50.9 % of the total), C(16 : 0) (17.9 %), 11 methyl C(18 : 1)omega7c (14.7 %), C(18 : 1)omega9c (2.9 %) and C(19 : 0) cyclo omega8c (2.4 %), and the predominant respiratory lipoquinone was ubiquinone-10. Based on physiological, chemotaxonomic and phylogenetic data, strain C02(T) is suggested to represent a novel species of a new genus, for which the name Tropicibacter naphthalenivorans gen. nov., sp. nov. is proposed. The type strain of Tropicibacter naphthalenivorans is C02(T) (=JCM 14838(T)=DSM 19561(T)).
Subject(s)
Polycyclic Aromatic Hydrocarbons/metabolism , Rhodobacteraceae/classification , Seawater/microbiology , Fatty Acids/analysis , Indonesia , Molecular Sequence Data , Phenotype , Phylogeny , RNA, Ribosomal, 16S , Rhodobacteraceae/chemistry , Rhodobacteraceae/genetics , Rhodobacteraceae/metabolism , Rhodobacteraceae/ultrastructure , Species SpecificityABSTRACT
An aerobic, Gram-negative, motile bacterium, strain B51(T), was isolated from seawater obtained from Semarang Port in Indonesia. Cells of strain B51(T) were peritrichously flagellated and rod-shaped. Strain B51(T) was able to degrade alkanes, branched alkanes and alkylnaphthalenes. 16S rRNA gene sequence analysis revealed that strain B51(T) was affiliated with the family Rhodobacteraceae, and was related most closely to Thioclava pacifica TL 2(T) (94.6 % similarity). The DNA G+C content of strain B51(T) was 66.5 mol%. The major cellular fatty acids were C(18 : 1)omega7c (84.9 %), C(18 : 1)omega9c (13.8 %), C(16 : 0) (8.7 %), C(18 : 0) (6.4 %) and anteiso-C(15 : 0) (5.8 %) and the major quinone was ubiquinone-10. Based on its phenotypic and phylogenetic characteristics, strain B51(T) is considered to represent a novel species of a new genus, for which the name Tropicimonas isoalcanivorans gen. nov., sp. nov. is proposed. The type strain of the type species is B51(T) (=JCM 14837(T)=DSM 19548(T)).
Subject(s)
Alkanes/metabolism , Rhodobacteraceae/classification , Seawater/microbiology , Microscopy, Electron, Transmission , Molecular Sequence Data , Phylogeny , RNA, Ribosomal, 16S/genetics , Rhodobacteraceae/genetics , Rhodobacteraceae/metabolism , Rhodobacteraceae/ultrastructure , Species SpecificityABSTRACT
Strain A34(T), an obligately halophilic, Gram-negative, non-motile, rod-shaped bacterium, was isolated from seawater obtained from Semarang Port in Indonesia. It possesses a pink pigment and degrades short-chain alkanes. It is positive for catalase and oxidase and reduces nitrate to nitrite. Analyses of 16S rRNA gene sequences revealed a clear affiliation of this strain with the family 'Rhodobacteraceae' in the class Alphaproteobacteria, with its closest relatives being Salipiger mucosus A3(T) (94.9% sequence similarity) and Palleronia marisminoris B33(T) (93.4%). The DNA G+C content was 69.1 mol%. The major cellular fatty acids of strain A34(T) were C(18:1)omega7c (56.2%), C(19:0) cyclo omega8c (26.0%) and C(16:0) (9.1%), while the predominant respiratory lipoquinone was ubiquinone-10. Based on the physiological and phylogenetic data, it is proposed that strain A34(T) should be classified in a new genus and species, for which the name Tranquillimonas alkanivorans gen. nov., sp. nov. is proposed. The type strain of Tranquillimonas alkanivorans is strain A34(T) (=JCM 14836(T) =DSM 19547(T)).
