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Antimicrob Agents Chemother ; 56(5): 2735-8, 2012 May.
Article in English | MEDLINE | ID: mdl-22354295

ABSTRACT

Clinical isolates of Klebsiella pneumoniae producing NDM-1 carbapenemase from India (n = 22), the United Kingdom (n = 13), and Sweden (n = 4) were subjected to multilocus sequence typing (MLST), automated repetitive sequence-based PCR (rep-PCR), serotyping, virulence gene screening, and plasmid replicon typing. The most frequently detected MLST sequence types (STs) were ST14 (n = 13; all serotype K2), ST11, ST149, ST231, and ST147. The correlation between MLST and automated rep-PCR was excellent. IncA/C was the most frequently detected plasmid replicon type (n = 14). ST14, ST11, and other successful clones may be important for the dissemination of bla(NDM-1).


Subject(s)
Klebsiella pneumoniae/genetics , beta-Lactam Resistance/genetics , beta-Lactamases/genetics , Electrophoresis, Gel, Pulsed-Field , Gene Transfer, Horizontal , Humans , India , Klebsiella pneumoniae/isolation & purification , Klebsiella pneumoniae/pathogenicity , Multilocus Sequence Typing , Phylogeography , Plasmids/genetics , Polymerase Chain Reaction , Replicon/genetics , Serotyping , Sweden , United Kingdom
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