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1.
FEBS Lett ; 592(12): 2048-2058, 2018 06.
Article in English | MEDLINE | ID: mdl-29772612

ABSTRACT

We have studied a series of human acetyl-CoA carboxylase (ACC) 1 and ACC2 proteins with deletions and/or Ser to Ala substitutions of the known phosphorylation sites. In vitro dephosphorylation/phosphorylation experiments reveal a substantial level of phosphorylation of human ACCs produced in insect cells. Our results are consistent with AMPK phosphorylation of Ser29 , Ser80 , Ser1,201 , and Ser1,216 . Phosphorylation of the N-terminal regulatory domain decreases ACC1 activity, while phosphorylation of residues in the ACC central domain has no effect. Inhibition of the activity by phosphorylation is significantly more profound at citrate concentrations below 2 mm. Furthermore, deletion of the N-terminal domain facilitates structural changes induced by citrate, including conversion of ACC dimers to linear polymers. We have also identified ACC2 amino acid mutations affecting specific inhibition of the isozyme by compound CD-017-0191. They form two clusters separated by 60-90 Å: one located in the vicinity of the BC active site and the other one in the vicinity of the ACC1 phosphorylation sites in the central domain, suggesting a contribution of the interface of two ACC dimers in the polymer to the inhibitor binding site.


Subject(s)
Acetyl-CoA Carboxylase/chemistry , Acetyl-CoA Carboxylase/metabolism , AMP-Activated Protein Kinases/metabolism , Acetyl-CoA Carboxylase/genetics , Amino Acid Substitution , Animals , Catalytic Domain/drug effects , Citric Acid/pharmacology , Crystallography, X-Ray , Humans , Models, Molecular , Phosphorylation , Protein Domains , Sequence Deletion , Sf9 Cells
2.
Proc Natl Acad Sci U S A ; 114(8): E1405-E1412, 2017 02 21.
Article in English | MEDLINE | ID: mdl-28159891

ABSTRACT

Channels that cross cell walls and connect the cytoplasm of neighboring cells in multicellular cyanobacteria are pivotal for intercellular communication. We find that the product of the gene all1140 of the filamentous cyanobacterium Anabaena sp. PCC 7120 is required for proper channel formation. All1140 encodes an amidase that hydrolyses purified peptidoglycans. An All1140-GFP fusion protein is located at the Z-ring in the periplasmic space during most of the cell cycle. An all1140-null mutant (M40) was unable to grow diazotrophically, and no mature heterocysts were observed in the absence of combined nitrogen. Expression of two key genes, hetR and patS, was studied in M40 using GFP as a reporter. Upon nitrogen step-down, the patterned distribution of green fluorescent cells in filaments seen in the wild type were not observed in mutant M40. Intercellular communication in M40 was studied by measuring fluorescence recovery after photobleaching (FRAP). Movement of calcein (622 Da) was aborted in M40, suggesting that the channels connecting the cytoplasm of neighboring cells are impaired in the mutant. The channels were examined with electron tomography; their diameters were nearly identical, 12.7 nm for the wild type and 12.4 nm for M40, suggesting that AmiC3 is not required for channel formation. However, when the cell wall sacculi isolated by boiling were examined by EM, the average sizes of the channels of the wild type and M40 were 20 nm and 12 nm, respectively, suggesting that the channel walls of the wild type are expandable and that this expandability requires AmiC3.


Subject(s)
Amidohydrolases/metabolism , Anabaena/metabolism , Cell Communication/physiology , Cyanobacteria/metabolism , Bacterial Proteins/metabolism , Cell Wall/metabolism , Cytoplasm/metabolism , Fluorescence Recovery After Photobleaching/methods , Gene Expression Regulation, Bacterial/physiology , Green Fluorescent Proteins/metabolism , Nitrogen/metabolism , Peptidoglycan/metabolism , Periplasm/metabolism
3.
Proc Natl Acad Sci U S A ; 112(32): E4458-64, 2015 Aug 11.
Article in English | MEDLINE | ID: mdl-26216997

ABSTRACT

The filamentous nitrogen-fixing cyanobacterium Anabaena sp. PCC 7120 differentiates specialized cells, heterocysts, that fix atmospheric nitrogen and transfer the fixed nitrogen to adjacent vegetative cells. Reciprocally, vegetative cells transfer fixed carbon to heterocysts. Several routes have been described for metabolite exchange within the filament, one of which involves communicating channels that penetrate the septum between adjacent cells. Several fra gene mutants were isolated 25 y ago on the basis of their phenotypes: inability to fix nitrogen and fragmentation of filaments upon transfer from N+ to N- media. Cryopreservation combined with electron tomography were used to investigate the role of three fra gene products in channel formation. FraC and FraG are clearly involved in channel formation, whereas FraD has a minor part. Additionally, FraG was located close to the cytoplasmic membrane and in the heterocyst neck, using immunogold labeling with antibody raised to the N-terminal domain of the FraG protein.


Subject(s)
Anabaena/cytology , Anabaena/metabolism , Bacterial Proteins/metabolism , Nitrogen Fixation , Anabaena/ultrastructure , Mutation/genetics , Subcellular Fractions/metabolism , Tomography
4.
Microbiology (Reading) ; 161(Pt 1): 84-88, 2015 Jan.
Article in English | MEDLINE | ID: mdl-25378560

ABSTRACT

Cyanobacteria use a sophisticated system of pigments to collect light energy across the visible spectrum for photosynthesis. The pigments are assembled in structures called phycobilisomes, composed of phycoerythrocyanin, phycocyanin and allophycocyanin, which absorb energy and transfer it to chlorophyll in photosystem II reaction centres. All of the components of this system are fluorescent, allowing sensitive measurements of energy transfer using single cell confocal fluorescence microscopy. The native pigments can be interrogated without the use of reporters. Here, we use confocal fluorescence microscopy to monitor changes in the efficiency of energy transfer as single cells age, between the time they are born at cell division until they are ready to divide again. Alteration of fluorescence was demonstrated to change with the age of the cyanobacterial cell.


Subject(s)
Anabaena/cytology , Anabaena/physiology , Microscopy, Confocal , Spectrometry, Fluorescence
5.
FASEB J ; 28(7): 3016-22, 2014 Jul.
Article in English | MEDLINE | ID: mdl-24675362

ABSTRACT

Cyanobacteria, formerly called blue-green algae, are abundant bacteria that carry out green plant photosynthesis, fixing CO2 and generating O2. Many species can also fix N2 when reduced nitrogen sources are scarce. Many studies imply the existence of intracellular communicating channels in filamentous cyanobacteria, in particular, the nitrogen-fixing species. In a species such as Anabaena, growth in nitrogen-depleted medium, in which ∼10% of the cells differentiate into anaerobic factories for nitrogen fixation (heterocysts), requires the transport of amino acids from heterocysts to vegetative cells, and reciprocally, the transport of sugar from vegetative cells to heterocysts. Convincing physical evidence for such channels has been slim. Using improved preservation of structure by high-pressure rapid freezing of samples for electron microscopy, coupled with high-resolution 3D tomography, it has been possible to visualize and measure the dimensions of channels that breach the peptidoglycan between vegetative cells and between heterocysts and vegetative cells. The channels appear to be straight tubes, 21 nm long and 14 nm in diameter for the latter and 12 nm long and 12 nm in diameter for the former.-Omairi-Nasser, A., Haselkorn, R., Austin, J. II. Visualization of channels connecting cells in filamentous nitrogen-fixing cyanobacteria.


Subject(s)
Cyanobacteria/ultrastructure , Intercellular Junctions/ultrastructure , Nitrogen Fixation/physiology , Nitrogen/metabolism , Thylakoids/ultrastructure , Cyanobacteria/metabolism , Cyanobacteria/physiology , Imaging, Three-Dimensional/methods , Intercellular Junctions/metabolism , Intercellular Junctions/physiology , Thylakoids/metabolism , Thylakoids/physiology
7.
Proc Natl Acad Sci U S A ; 110(19): E1716-23, 2013 May 07.
Article in English | MEDLINE | ID: mdl-23610410

ABSTRACT

HetR is an essential regulator of heterocyst development in cyanobacteria. Many mutations in HetR render Anabaena incapable of nitrogen fixation. The protein binds to a DNA palindrome upstream of hetP and other genes. We have determined the crystal structures of HetR complexed with palindromic DNA targets, 21, 23, and 29 bp at 2.50-, 3.00-, and 3.25-Å resolution, respectively. The highest-resolution structure shows fine details of specific protein-DNA interactions. The lower-resolution structures with longer DNA duplexes have similar interaction patterns and show how the flap domains interact with DNA in a sequence nonspecific fashion. Fifteen of 15 protein-DNA contacts predicted on the basis of the structure were confirmed by single amino acid mutations that abolished binding in vitro and complementation in vivo. A striking feature of the structure is the association of glutamate 71 from each subunit of the HetR dimer with three successive cytosines in each arm of the palindromic target, a feature that is conserved among all known heterocyst-forming cyanobacteria sequenced to date.


Subject(s)
Anabaena/metabolism , Bacterial Proteins/metabolism , Cyanobacteria/genetics , DNA, Bacterial/chemistry , Transcription Factors/metabolism , Amino Acids/chemistry , Anabaena/genetics , Crystallography, X-Ray , Cyanobacteria/metabolism , Cytosine/chemistry , Genetic Complementation Test , Glutamic Acid/chemistry , Inverted Repeat Sequences , Models, Molecular , Molecular Conformation , Mutagenesis , Mutation , Nitrogen/chemistry , Protein Binding , Sequence Analysis, DNA
8.
J Bacteriol ; 195(1): 25-8, 2013 Jan.
Article in English | MEDLINE | ID: mdl-23086210

ABSTRACT

The filamentous cyanobacterium Anabaena fixes nitrogen in specialized cells called heterocysts. The immediate product of fixation, ammonia, is known to be assimilated by addition to glutamate to make glutamine. How fixed nitrogen is transported along the filament to the 10 to 20 vegetative cells that separate heterocysts is unknown. N-fixing heterocysts accumulate an insoluble polymer containing aspartate and arginine at the cell poles. Lockau's group has proposed that the polymer is degraded at the poles to provide a mobile carrier, arginine, to the vegetative cells (R. Richter, M. Hejazi, R. Kraft, K. Ziegler, and W. Lockau, Eur. J. Biochem. 263:163-169, 1999). We wished to use the Sakaguchi reaction for arginine to determine the relative cellular concentration of arginine along the filament. At present, the methods for measuring absorption of the Sakaguchi reaction product at 520 nm are insufficiently sensitive for that purpose. However, that product quenches the fluorescence of phycobiliproteins, which we have adapted to a determination of arginine. Our results are consistent with the proposal that arginine is a principal nitrogen carrier from heterocysts to vegetative cells in Anabaena.


Subject(s)
Anabaena/chemistry , Anabaena/cytology , Arginine/chemistry , Phycobilisomes/chemistry , Ammonia/metabolism , Anabaena/metabolism , Arginine/metabolism , Bacterial Proteins/genetics , Bacterial Proteins/metabolism , Biological Transport, Active , Cytoskeleton/physiology , Fluorescence , Gene Expression Regulation, Bacterial/physiology , Glutamic Acid/metabolism , Glutamine/metabolism , Nitrogen Fixation , Phycobilisomes/metabolism , Spectrometry, Fluorescence
9.
Microbiology (Reading) ; 159(Pt 2): 253-258, 2013 Feb.
Article in English | MEDLINE | ID: mdl-23223442

ABSTRACT

Filamentous Anabaena PCC 7120 differentiates nitrogen-fixing specialized cells called heterocysts at regular intervals following removal of combined nitrogen from the medium. Phycobiliproteins are degraded during differentiation. Heterocyst differentiation was followed at the single cell level by using confocal fluorescence microscopy. The presence of an enhanced fluorescence emission peak from allophycocyanin (APC) indicates that the degradation of the phycobilisomes during nitrogen deprivation possibly initiates at the linker between APC and photosystem II in a bottom-to-top disassembly model. Furthermore, the fluorescence emission peak around 650 nm provides an advantageous marker to identify early candidates for differentiation.


Subject(s)
Anabaena/chemistry , Anabaena/cytology , Phycocyanin/analysis , Spectrometry, Fluorescence , Phycobilisomes/metabolism
13.
Proc Natl Acad Sci U S A ; 108(25): 10109-14, 2011 Jun 21.
Article in English | MEDLINE | ID: mdl-21628585

ABSTRACT

HetR is an essential regulator of heterocyst development in cyanobacteria. HetR binds to a DNA palindrome upstream of the hetP gene. We report the crystal structure of HetR from Fischerella at 3.0 Å. The protein is a dimer comprised of a central DNA-binding unit containing the N-terminal regions of the two subunits organized with two helix-turn-helix motifs; two globular flaps extending in opposite directions; and a hood over the central core formed from the C-terminal subdomains. The flaps and hood have no structural precedent in the protein database, therefore representing new folds. The structural assignments are supported by site-directed mutagenesis and DNA-binding studies. We suggest that HetR serves as a scaffold for assembly of transcription components critical for heterocyst development.


Subject(s)
Bacterial Proteins/chemistry , Cyanobacteria/physiology , Protein Structure, Quaternary , Protein Structure, Tertiary , Transcription Factors/chemistry , Bacterial Proteins/genetics , Bacterial Proteins/metabolism , Crystallography, X-Ray , Cyanobacteria/cytology , Cyanobacteria/genetics , DNA, Bacterial/metabolism , Gene Expression Regulation, Bacterial , Models, Molecular , Molecular Sequence Data , Mutagenesis, Site-Directed , Protein Binding , Transcription Factors/genetics , Transcription Factors/metabolism
14.
J Bacteriol ; 192(18): 4732-40, 2010 Sep.
Article in English | MEDLINE | ID: mdl-20622060

ABSTRACT

The filamentous cyanobacterium Anabaena sp. strain PCC 7120 forms a periodic pattern of nitrogen-fixing heterocysts when grown in the absence of combined nitrogen. PatA is necessary for proper patterning of heterocysts along filaments. In this study, apparent transcriptional start points (tsps) were identified at nucleotides -305, -614, and -645 relative to the translational start site (-305, -614, and -645 tsps). Transcriptional reporter fusions were used to show that transcription from the -305 tsp was induced in all cells of filaments in response to nitrogen deprivation, required hetR for induction, and increased in a patA mutant. Transcription from -614/-645 tsp reporter fusions was spatially regulated and occurred primarily in cells that would become heterocysts. Complementation of a patA mutant strain by alleles encoding substitutions in, or deletion of, the putative phosphoacceptor C-terminal domain indicates that the PATAN domain can function independently of the C-terminal domain of PatA. Localization of a ring of PatA-GFP at sites of cell division, as well as the formation of enlarged cells with altered cell morphology when patA was overexpressed, suggests that PatA may participate in cell division.


Subject(s)
Anabaena/cytology , Anabaena/genetics , Bacterial Proteins/genetics , Gene Expression Regulation, Bacterial , Transcription, Genetic/genetics , Gene Expression Regulation, Bacterial/genetics , Gene Expression Regulation, Bacterial/physiology , Nucleic Acid Amplification Techniques , Polymerase Chain Reaction , Promoter Regions, Genetic/genetics
15.
PLoS One ; 5(7): e11486, 2010 Jul 08.
Article in English | MEDLINE | ID: mdl-20628610

ABSTRACT

BACKGROUND: An ancient cyanobacterial incorporation into a eukaryotic organism led to the evolution of plastids (chloroplasts) and subsequently to the origin of the plant kingdom. The underlying mechanism and the identities of the partners in this monophyletic event remain elusive. METHODOLOGY/PRINCIPAL FINDINGS: To shed light on this evolutionary process, we sequenced the genome of a cyanobacterium residing extracellularly in an endosymbiosis with a plant, the water-fern Azolla filiculoides Lam. This symbiosis was selected as it has characters which make it unique among extant cyanobacterial plant symbioses: the cyanobacterium lacks autonomous growth and is vertically transmitted between plant generations. Our results reveal features of evolutionary significance. The genome is in an eroding state, evidenced by a large proportion of pseudogenes (31.2%) and a high frequency of transposable elements (approximately 600) scattered throughout the genome. Pseudogenization is found in genes such as the replication initiator dnaA and DNA repair genes, considered essential to free-living cyanobacteria. For some functional categories of genes pseudogenes are more prevalent than functional genes. Loss of function is apparent even within the 'core' gene categories of bacteria, such as genes involved in glycolysis and nutrient uptake. In contrast, serving as a critical source of nitrogen for the host, genes related to metabolic processes such as cell differentiation and nitrogen-fixation are well preserved. CONCLUSIONS/SIGNIFICANCE: This is the first finding of genome degradation in a plant symbiont and phenotypically complex cyanobacterium and one of only a few extracellular endosymbionts described showing signs of reductive genome evolution. Our findings suggest an ongoing selective streamlining of this cyanobacterial genome which has resulted in an organism devoted to nitrogen fixation and devoid of autonomous growth. The cyanobacterial symbiont of Azolla can thus be considered at the initial phase of a transition from free-living organism to a nitrogen-fixing plant entity, a transition process which may mimic what drove the evolution of chloroplasts from a cyanobacterial ancestor.


Subject(s)
Cyanobacteria/growth & development , Cyanobacteria/genetics , Ferns/microbiology , Genome, Bacterial/genetics , Symbiosis/physiology , Biological Evolution , Nitrogen Fixation/genetics , Nitrogen Fixation/physiology , Symbiosis/genetics
16.
Mol Microbiol ; 77(3): 537-9, 2010 Aug.
Article in English | MEDLINE | ID: mdl-20545863

ABSTRACT

Heterocysts are terminally differentiated cells that fix nitrogen in filaments of the cyanobacterium Anabaena PCC 7120. They differentiate from vegetative cells at regular intervals along each filament. The developmental process is initiated by an increase in the ratio of reduced carbon to reduced nitrogen. This cue triggers protein NtcA to activate transcription of nrrA, which leads to transcription of the hetR gene. HetR is a master transcription factor required for expression of many heterocyst-specific genes. One such gene is hetP, shown by Higa and Callahan in this edition of Molecular Microbiology to be able to replace hetR for most of the downstream events required for a functional heterocyst. Ectopic production of HetP in a hetR mutant allows the differentiation of heterocysts. These heterocysts can fix nitrogen under anaerobic conditions but they are unable to provide wild-type protection of nitrogenase from oxygen, so they cannot bypass all of the duties of HetR. Additionally, the 5'-flanking region of the hetP gene provides the best-characterized binding site for the HetR protein so far, a seven-base pair inverted repeat.


Subject(s)
Anabaena/growth & development , Anabaena/metabolism , Bacterial Proteins/genetics , Bacterial Proteins/metabolism , Gene Expression Regulation, Bacterial , Amino Acid Motifs , Anabaena/genetics , Bacterial Proteins/chemistry , Cyanobacteria/chemistry , Cyanobacteria/genetics , Cyanobacteria/growth & development , Cyanobacteria/metabolism , Protein Binding
17.
Proc Natl Acad Sci U S A ; 107(20): 9093-8, 2010 May 18.
Article in English | MEDLINE | ID: mdl-20439761

ABSTRACT

Acetyl-CoA carboxylase (ACC) is a key enzyme of fatty acid metabolism with multiple isozymes often expressed in different eukaryotic cellular compartments. ACC-made malonyl-CoA serves as a precursor for fatty acids; it also regulates fatty acid oxidation and feeding behavior in animals. ACC provides an important target for new drugs to treat human diseases. We have developed an inexpensive nonradioactive high-throughput screening system to identify new ACC inhibitors. The screen uses yeast gene-replacement strains depending for growth on cloned human ACC1 and ACC2. In "proof of concept" experiments, growth of such strains was inhibited by compounds known to target human ACCs. The screen is sensitive and robust. Medium-size chemical libraries yielded new specific inhibitors of human ACC2. The target of the best of these inhibitors was confirmed with in vitro enzymatic assays. This compound is a new drug chemotype inhibiting human ACC2 with 2.8 muM IC(50) and having no effect on human ACC1 at 100 muM.


Subject(s)
Acetyl-CoA Carboxylase/antagonists & inhibitors , Drug Discovery/methods , Enzyme Inhibitors/isolation & purification , Fatty Acids/metabolism , Obesity/drug therapy , Acetyl-CoA Carboxylase/genetics , Acetyl-CoA Carboxylase/metabolism , DNA, Complementary/genetics , Dose-Response Relationship, Drug , Enzyme Inhibitors/pharmacology , Gene Components , Humans , Inhibitory Concentration 50 , Isoenzymes/antagonists & inhibitors , Isoenzymes/genetics , Isoenzymes/metabolism , Organisms, Genetically Modified , Yeasts
18.
J Bacteriol ; 192(13): 3545-6, 2010 Jul.
Article in English | MEDLINE | ID: mdl-20418398

ABSTRACT

Rhodobacter capsulatus SB 1003 belongs to the group of purple nonsulfur bacteria. Its genome consists of a 3.7-Mb chromosome and a 133-kb plasmid. The genome encodes genes for photosynthesis, nitrogen fixation, utilization of xenobiotic organic substrates, and synthesis of polyhydroxyalkanoates. These features made it a favorite research tool for studying these processes. Here we report its complete genome sequence.


Subject(s)
Genome, Bacterial/genetics , Rhodobacter capsulatus/genetics , Open Reading Frames/genetics
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