Antioxidant-rich brilliant polymeric nanocomposites for quick and efficient non-enzymatic hydrogen peroxide sensor.

In our current research, a new type of functional nanocomposites known as poly(methyl methacrylate/N,N-dimethyl aminoethylmethacrylate/(E)-2-cyano-N-cyclohexyl-3 (dimethylamino) acrylamide) [poly(MMA/DMAEMA/CHAA)] has been developed. These nanocomposites were created using microemulsion polymerization in conjunction with synthesized titanium dioxide (TiO2), and vanadium pentoxide (V2O5) nanoparticles. To understand the physio-chemical characteristics of the poly(MMA/DMAEMA/CHAA) and the metal oxide nanoparticles (MOs) integrated within them, various analytical techniques were employed. These techniques included Fourier-transform infrared spectroscopy (FT-IR), proton nuclear magnetic resonance (1H NMR), X-ray diffraction analysis (XRD), thermogravimetric analysis (TGA), scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDX), transmission electron microscopy (TEM), and electrical approaches such as cyclic voltammetry (CV) and electrical impedance spectra (EIS). Based on the TEM results, nanospheres with a well-defined structure were developed for both the pure polymer and its composite with sizes ranging from 45 to 75 nm. All the TiO2 and V2O5-based nanocomposites showed significantly enhanced electrical attributes, with capacitance values surpassing those of the poly(MMA/DMAEMA/CHAA) nanosphere assemblies by a considerable margin. As a result, both direct electron transfer and direct hydrogen peroxide identification were evaluated for the nanocomposites. The amperometry results demonstrated a lower detection limit of 0.0085 µM and a rapid linear sensitivity in the range of 1 to 800 µM. The greatly improved electrolytic qualities of these nanocomposites make them suitable for various applications in fields such as battery storage, sensors, and biosensors.

Designing a green poly(ß-amino ester) for the delivery of nicotinamide drugs with biological activities and conducting a DFT investigation.

The environmentally friendly polymerization process was carried out using microwave irradiation without additional solvents or catalysts to produce poly(ß-amino ester) (PßAE) which served as a drug delivery system. PßAE was synthesized through Michael addition polymerization of 1,4-butane diol diacrylate and piperazine. Swelling and biodegradation studies were conducted in various solvents and phosphate-buffered saline (PBS, pH 7.4) at 37 °C to evaluate the properties of the polymeric gel. The PßAE matrix demonstrated solubility enhancement for hydrophobic antimicrobial and antitumor-active nicotinamide derivatives (TEINH, APTAT, and MOAPM), controlling their release over 10 days in (PBS). The successful formation of free and loaded PßAE with nicotinamide active materials was confirmed by spectroscopic analysis including Fourier-transform infrared spectroscopy (FT-IR) and scanning electron microscopy (SEM). Optimization and physical descriptor determination via the DFT/B3LYP-631(G) basis set were performed to aid in the biological evaluation of these compounds with elucidation of their physical and chemical interaction between poly(ß-amino ester) and nicotinamide drugs.

Calcium alginate beads encapsulated PMMA-g-CS nano-particles for α-chymotrypsin immobilization.

Chitosan grafted with polymethyl methacrylate (PMMA-g-CS) was prepared via a free-radicals polymerization technique as a carrier for enzyme immobilization. α-Chymotrypsin (CT), as an enzyme model in this study, was immobilized onto the prepared PMMA-g-CS via covalent bonding. Calcium alginate (CA) beads were developed for encapsulating PMMA-g-CS-CT to produce PMMA-g-CS-CT/CA composite beads. Morphology and size of PMMA-g-CS particles were investigated by TEM and found to be in the nanoscale. The structure and surface morphology of the beads before and after immobilization process were characterized by FT-IR and SEM, respectively. Both the bound CT content and relative activity of immobilized enzyme were measured. A higher retained activity (about 97.7%) obtained for the immobilized CT at pH 9 for 24 h. The results indicated that immobilized CT maintained excellent performance even after 25 reuses and retained 75% from its original activity after 60 days of storage at 25 °C.

pH-sensitive sodium alginate hydrogels for riboflavin controlled release.

Sodium alginate (SA) grafted with polyglycidyl methacrylate hydrogels (PGMA-g-SA) was prepared as pH sensitive drug delivery matrices for riboflavin (RF). The hydrogel copolymer matrices were compared with calcium alginate (CA) beads for swelling, degradation, entrapment efficiency and in vitro release of RF. The structure, surface morphology of the CA beads and the prepared hydrogels as well as the chemical stability of the encapsulated drug were characterized by FT-IR and SEM, respectively. The results demonstrate that the optimal formulation was achieved with PGMA-g-SA proportion of (0.75 mol/1 g) and loaded RF 0.03 g. It has been observed that the in vitro release study of RF from this formulation was superior to the other ones and was able to maintain the release for ∼3 and 4 days for the simulated intestinal fluid (SIF) and simulated gastric fluid (SGF), respectively. In general, it has been shown that, GMA grafted onto SA enhanced drug entrapment efficiency, decreased swelling and degradation behaviors of the carrier. In addition, it slowed and controlled the release of RF from the PGMA-g-SA hydrogel compared with pure SA beads crosslinked with Ca2+ ions alone, which thereby provides a facile and effective method to improve the drug delivery systems.