ABSTRACT
A new highly green luminescent binuclear palladium 2-pyrazinecarboxamide-bipyridine complex [Pd(pyc)(bpy)] was prepared and characterized. The binuclear Pd(pyc)(bpy) complex doped in sol-gel matrix has a strong luminescence intensity at 547â¯nm with λex =â¯330â¯nm in water The method depends on the quenching of the luminescence intensity of the binuclear Pd(pyc)(bpy) complex at 547â¯nm by different concentrations of uric acid. The remarkable quenching of the luminescence intensity of the binuclear Pd(pyc)(bpy) complex, doped in a sol-gel matrix, by uric acid was successfully used for the determination of uric acid in serum samples of patients with hypouricemia disease. The calibration plot was achieved over the concentration 3.9â¯×â¯10-9 to 1.2â¯×â¯10-4 mol L-1uric acid with a correlation coefficient of 0.9 and a detection limit of 1.8â¯×â¯10-10 mol L-1. The method was used satisfactorily for the assessment of the uric acid in a number of serum samples collected from various patients with Hypouricemia disease.
Subject(s)
2,2'-Dipyridyl/analogs & derivatives , 2,2'-Dipyridyl/chemistry , Coordination Complexes/chemistry , Optical Devices , Palladium/chemistry , Pyrazines/chemistry , Uric Acid/blood , 2,2'-Dipyridyl/chemical synthesis , Carcinoma, Hepatocellular/blood , Cardiovascular Diseases/blood , Coordination Complexes/chemical synthesis , Gels , Humans , Liver Neoplasms/blood , Optical Imaging , Pyrazines/chemical synthesisABSTRACT
A novel, simple, sensitive and selective spectrofluorimetric method was developed for the determination of trace amounts of chlorzoxazone and Ibuprofen in pharmaceutical tablets using optical sensor Eu-Tetracycline HCl doped in sol-gel matrix. The chlorzoxazone or Ibuprofen can remarkably enhance the luminescence intensity of Eu-Tetracycline HCl complex doped in a sol-gel matrix in dimethylformamide (DMF) at pH 9.7 and 6.3, respectively, λ(ex) = 400 nm. The enhancing of luminescence intensity peak of Eu-Tetracycline HCl complex at 617 nm is proportional to the concentration of chlorzoxazone or Ibuprofen a result that suggested profitable application as a simple optical sensor for chlorzoxazone or Ibuprofen assessment. The dynamic ranges found for the determination of chlorzoxazone and Ibuprofen concentration are 5 × 10(-9)-1 × 10(-4) and 1 × 10(-8)-7 × 10(-5) mol L(-1), and the limit of detection (LOD) and quantitation limit of detection (LOQ) are 3.1 × 10(-10), 9.6 × 10(-10) and 5.6 × 10(-10), 1.7 × 10(-9) mol L(-1), respectively.
Subject(s)
Chlorzoxazone/analysis , Dimethylformamide/chemistry , Fluorescent Dyes/chemistry , Ibuprofen/analysis , Optical Devices , Organometallic Compounds/chemistry , Pharmaceutical Preparations/analysis , Spectrometry, Fluorescence/methods , Tetracyclines/chemistry , Gels/chemistry , Luminescence , Molecular Structure , Tablets/chemistryABSTRACT
A simple and sensitive spectrofluorimetric method for determination of trace amount of doxycycline hydrochloride (DC) in pharmaceutical tablets and serum samples was developed. In ammonia buffer solution of pH 8.9 the doxycycline hydrochloride can remarkably enhance the luminescence intensity of the Sm(3+) ion in Sm(3+)- DC complex at λ(ex)=400 nm. The produced luminescence intensity of Sm(3+)- DC complex in DMSO is in proportion to the concentration of DC and used as optical sensor for its determination. The dynamic range for the determination of DC is 1 × 10(-8)-5 × 10(-6) mol L(-1) and in case of quantum yield calculations is 7 × 10(-9)-5 × 10(-6) mol L(-1) with detection limit of 6.5 × 10(-10) mol L(-1). The enhancement mechanism of the luminescence intensity in the Sm(3+)- DC system has been also discussed. A comparison with other spectrofluorimetric methods for tetracycline derivatives in which Eu(3+) ion is used instead of Sm(3+) ion is also studied.
