Chitosan-polyethylene oxide/clay-alginate nanofiber hydrogel scaffold for bone tissue engineering: Preparation, physical characterization, and biomimetic mineralization.

This study aimed to prepare a novel organic-mineral nanofiber/hydrogel of chitosan-polyethylene oxide (CS-PEO)/nanoclay-alginate (NC-ALG). The effects of NC particles on the mineralization and biocompatibility of the scaffold were investigated. A layer-by-layer scaffold composed of CS-PEO and NC-ALG was prepared. The morphological properties, swelling, biodegradation, and mechanical behaviors of the scaffolds were evaluated. Furthermore, scaffolds were characterized by the Fourier Transform Infrared (FTIR), the Field Emission Scanning Electron Microscope (FE-SEM), and X-Ray Diffraction (XRD) techniques. Bone-like apatite formation ability of the scaffolds was determined by the mineralization test in a simulated body fluid (M-SBF). In addition, the crystalline phase of bone-like apatite precipitates was investigated by XRD analysis. The cell compatibility of the scaffolds was also studied with osteoblastic cell line MC3T3-E1 by MTT assay. Notably, the incorporation of NC particles in CS-PEO/ALG scaffolds is suitable for bone tissue regeneration which enhances bone-like apatite formation. Further, the hemolysis and MTT assays demonstrated that CS-PEO/NC-ALG scaffold was compatible and safe for MC3T3 cells.

Recent advances of polymer-based piezoelectric composites for biomedical applications.

Over the past decades, electronics have become central to many aspects of biomedicine and wearable device technologies as a promising personalized healthcare platform. Lead-free piezoelectric materials for converting mechanical into electrical energy through piezoelectric transduction are of significant value in a diverse range of technological applications. Organic piezoelectric biomaterials have attracted widespread attention as the functional materials in the biomedical devices due to their advantages of excellent biocompatibility. They include synthetic and biological polymers. Many biopolymers have been discovered to possess piezoelectricity in an appreciable amount, however their investigation is still preliminary. Due to their piezoelectric properties, better known synthetic fluorinated polymers have been intensively investigated and applied in biomedical applications including controlled drug delivery systems, tissue engineering, microfluidic and artificial muscle actuators, among others. Piezoelectric polymers, especially poly (vinylidene fluoride) (PVDF) and its copolymers are increasingly receiving interest as smart biomaterials due to their ability to convert physiological movements to electrical signals when in a controllable and reproducible manner. Despite possessing the greatest piezoelectric coefficients among all piezoelectric polymers, it is often desirable to increase the electrical outputs. The most promising routes toward significant improvements in the piezoelectric response and energy-harvesting performance of such materials is loading them with various inorganic nanofillers and/or applying some modification during the fabrication process. This paper offers a comprehensive review of the principles, properties, and applications of organic piezoelectric biomaterials (polymers and polymer/ceramic composites) with special attention on PVDF-based polymers and their composites in sensors, drug delivery and tissue engineering. Subsequently focuses on the most common fabrication routes to produce piezoelectric scaffolds, tissue and sensors which is electrospinning process. Promising upcoming strategies and new piezoelectric materials and fabrication techniques for these applications are presented to enable a future integration among these applications.

Fabrication of ciprofloxacin-loaded chitosan/polyethylene oxide/silica nanofibers for wound dressing application: In vitro and in vivo evaluations.

Silica plays an effective role in collagen creation; hence, the degradation products of silica-based materials accelerate wound healing. In this regard, chitosan/polyethylene oxide/silica hybrid nanofibers were prepared by the combining the sol-gel method with electrospinning technique to accelerate the wound healing process. Ciprofloxacin, as an antibacterial drug, was then added to the electrospinning mixture. The nanofibers were characterized by SEM, EDX, X-ray mapping, TEM, TGA, FTIR, and XRD analysis. The degradation, swelling ratio, and release of ciprofloxacin were investigated in PBS. The prepared nanofiber could absorb water, maintain its morphological integrity during the degradation process, and gradually release ciprofloxacin. The nanofibers revealed an efficient antibacterial activity against Escherichia coli and Staphylococcus aureus. Cell viability assays showed that the nanofibers had no cytotoxicity against L929 mouse fibroblast and HFFF2 human foreskin fibroblast cell lines. The potential of the chitosan/polyethylene oxide/silica/ciprofloxacin nanofiber for healing full-thickness wound was assessed by applying the scaffold in the dorsal cutaneous wounds of the Balb/C mice. The white blood cell counts of the animals indicated the nanofiber-treated mice compared with the untreated ones had less infection and inflammation. According to the histopathologic data, the prepared nanofiber accelerated and enhanced tissue regeneration by increasing fibroblast cells and angiogenesis as well as decreasing the inflammation phase. The findings suggest that the prepared antibacterial scaffold with drug delivery properties could be an appropriate candidate for many medical and hygienic applications, especially as a bio-compatible and bio-degradable wound dressing.

Antibacterial and anti-inflammatory drug delivery properties on cotton fabric using betamethasone-loaded mesoporous silica particles stabilized with chitosan and silicone softener.

In this study, mesoporous silica particles with a hexagonal structure (SBA-15) were synthesized and modified with (3-aminopropyl) triethoxysilane, and used as a carrier for anti-inflammatory drug, betamethasone sodium phosphate. Drug-loaded silica particles were grafted on the cotton fabric surface using chitosan and polysiloxane reactive softener as a soft and safe fixing agent to develop an antibacterial cotton fabric with drug delivery properties. Cytometry assays revealed that synthesized silica have no cytotoxicity against human peripheral blood mononuclear cells. Accordingly, the produced drug-loaded nanostructures can be applied via different routes, such as wound dressing. Drug delivery profile of the treated fabrics were investigated and compared. The drug release rate followed the conventional Higuchi model. The treated cotton fabrics were tested and evaluated using scanning electron microscope images, bending length, air permeability, washing durability and anti-bacterial properties. It was found that the chitosan-/softener-treated fabrics compounded with drug-loaded silica particles have a good drug delivery performance and exhibited a powerful antibacterial activity against both Escherichia coli and Staphylococcus aureus even after five washing cycles. The produced antibacterial cotton fabric with drug delivery properties could be proposed as a suitable material for many medical and hygienic applications.

A novel cotton fabric with anti-bacterial and drug delivery properties using SBA-15-NH2/polysiloxane hybrid containing tetracycline.

Here, mesoporous silica particles containing tetracycline were loaded on cotton fabric for possible application on the infected human skin. Amino functionalized mesoporous silica, SBA-15-NH2, was chosen as a safe drug carrier loaded with tetracycline via post impregnation method. Diverse content of the drug loaded silica particles were then attached on the cotton fabric surface using polysiloxane reactive softener as a soft and safe fixing agent. UV-Vis spectroscopy was used to study the drug delivery properties of the mesoporous silica on the treated cotton fabrics. The treated fabric with long drug release properties was selected as the optimized sample. Further analysis was carried out on this sample including anti-bacterial, water contact angle, bending length, mineral content and washing durability. Also, SEM images, EDX patterns, X-Ray spectra and thermal behavior of the optimum sample were studied. The optimized treated sample indicated the gradual release profile of tetracycline in PBS buffer media within 48h along with excellent anti-bacterial efficiency as a good feature for biological application.

Optimization of tetracycline hydrochloride adsorption on amino modified SBA-15 using response surface methodology.

Several researchers are focused on preparation of mesoporous silica as drug carriers with high loading efficiency to control or sustain the drug release. Carriers with highly loaded drug are utilized to minimize the time of drug intake. In this study, amino modified SBA-15 was synthesized through grafting with amino propyl triethoxy silane and then loaded with tetracycline hydrochloride. The drug loading was optimized by using the response surface method considering various factors including drug to silica ratio, operation time, and temperature. The drug to silica ratio indicated as the most influential factor on the drug loading yield. Further, a quadratic polynomial equation was developed to predict the loading percentage. The experimental results indicated reasonable agreement with the predicted values. The modified and drug loaded mesoporous particles were characterized by FT-IR, SEM, TEM, X-ray diffraction (XRD), elemental analysis and N2 adsorption-desorption. The release profiles of tetracycline-loaded particles were studied in different pH. Also, Higuchi equation was used to analyze the release profile of the drug and to evaluate the kinetic of drug release. The drug release rate followed the conventional Higuchi model that could be controlled by amino-functionalized SBA-15. Further, the drug delivery system based on amino modified SBA-15 exhibits novel features with an appropriate usage as an anti-bacterial drug delivery system with effective management of drug adsorption and release.

Synthesis and surface modification of mesoporous silica nanoparticles and its application as carriers for sustained drug delivery.

High drug loading is one of the important issues in the drug delivery research, especially the drug delivery system by oral administration. If high drug loading carriers are utilized the times of drug intake could be significantly reduced. Accordingly in this study, ordered mesoporous SBA-15 modified with (3-aminopropyl) triethoxysilane (APTES) was used as a carrier for nonsteroidal anti-inflammatory drug and optimization of the loading process was done. SBA-15 silica material with rope-like morphology was synthesized and modified by post-synthesis method with APTES. The synthesized SBA-15 and modified SBA-15 were characterized by XRD, SEM, thermal analysis and FT-IR spectroscopy. Loading optimization experiments were performed by changing the factors affecting the drug loading, such as temperature, time, stirring rate, Ibuprofen/SBA-15 ratio. The results of drug delivery experiments showed that the surface modification of SBA-15 with amino groups significantly increases the drug loading and decreases the drug delivery rate.