ABSTRACT
The essential oil of aerial parts, leaves and flowers of the endemic Anthemis aciphylla BOISS. var. discoidea BOISS. (Asteraceae) were obtained by hydrodistillation. The oils were analyzed both by GC and GC-MS on a polar column. The monoterpenes alpha-pinene (9-49%) and terpinen-4-ol (22-32%) were characterized as the main constituents. An unknown component isolated from the essential oil was characterized by means of MS, HR-MS, FT-IR, 1D- and 2D-NMR techniques as isofaurinone (1). Furthermore, the biological activity of the essential oils was evaluated in various human pathogenic microorganisms using the broth microdilution method. Weak to moderate inhibitions (0.06-1.0 mg/ml) was observed.
Subject(s)
Anthemis/chemistry , Anti-Infective Agents/chemistry , Anti-Infective Agents/pharmacology , Oils, Volatile/chemistry , Oils, Volatile/pharmacology , Bacteria/drug effects , Candida albicans/drug effects , Chromatography, Gas , Gas Chromatography-Mass Spectrometry , Magnetic Resonance Spectroscopy , Microbial Sensitivity Tests , Spectrophotometry, Infrared , Staphylococcus epidermidis/drug effectsABSTRACT
Essential oil obtained by micro-distillation and hydrodistillation of the endemic Ferulago thirkeana (Boiss.) Boiss. (Apiaceae) was analysed by GC/MS. An unknown component isolated from the essential oil was characterized by chromato-spectral techniques (1D-, 2D-NMR, HRMS, IR and UV) as (1 S)-2,6,6-trimethyl-4-oxobicyclo[3.1.1]hept-2-enyl(2 E)-2-methylbut-2-enoate (= ferulagone or 1-angeloyloxyverbenone) (1). Biological activities of the essential oil and the purified major component (1) were tested against various human pathogenic microorganisms resulting in moderate inhibition (62.5 - 125 microg/ml).
