ABSTRACT
The object of this study was to determine the correlation between clinical symptoms and the activity of enzymes such as collagenase, chondroitinase, and hyaluronidase produced by bacteria isolated from infected root canals. The materials examined consisted of 28 teeth with apical periodontitis from 25 patients. Bacteria producing collagenase or chondroitinase and hyaluronidase were found to be significantly related to subacute clinical symptoms involving percussion pain. The frequency of bacteria producing collagenase was higher in isolates from root canals with a radiolucent area over 5 mm in diameter than in those from canals having a radiolucent area less than 5 mm in diameter.
Subject(s)
Bacteria/enzymology , Bacterial Infections/enzymology , Periapical Periodontitis/enzymology , Periapical Periodontitis/microbiology , Adult , Bacteroides/enzymology , Chondroitinases and Chondroitin Lyases/metabolism , Colony Count, Microbial , Eubacterium/enzymology , Female , Gram-Positive Bacteria/enzymology , Humans , Hyaluronoglucosaminidase/metabolism , Male , Microbial Collagenase/metabolism , Peptococcus/enzymology , Porphyromonas gingivalis/enzymology , Toothache/microbiologyABSTRACT
The purpose of this study was to investigate the correlation between the composition of bacterial flora from infected root canals and clinical symptoms. The materials evaluated consisted of 28 teeth from 25 patients with apical periodontitis. Eubacterium were found to be significantly related to acute or chronic clinical symptoms and Peptococcus, Peptostreptococcus, and Porphyromonas gingivalis to subacute clinical symptoms. We suggested that Peptococcus, Peptostreptococcus, Eubacterium, Porphyromonas, and Bacteroides were significantly related to percussion pain; Porphyromonas and Bacteroides were significantly related to odor in the infected root canals. Many Bacteroides were isolated from most of the infected root canals.
Subject(s)
Bacteria, Anaerobic/isolation & purification , Bacterial Infections/microbiology , Dental Pulp Cavity/microbiology , Periapical Periodontitis/microbiology , Adult , Bacteroides/isolation & purification , Dental Pulp Cavity/physiopathology , Eubacterium/isolation & purification , Female , Humans , Male , Peptococcus/isolation & purification , Peptostreptococcus/isolation & purification , Periapical Periodontitis/physiopathologyABSTRACT
The purpose of this study was to measure the amount of endotoxin as well as to identify Gram-negative bacteria in experimental periapical lesions in rats. Molar pulps were exposed and infected and the amount of endotoxin in the periapical tissue of the right mandibular first molar was measured by Endospecy, while the colony number of Gram-negative bacteria was determined in the same region of the left mandibular first molar. In the control animals, the amount of endotoxin in the periapical tissues did not change at all during the experimental period, and no Gram-negative bacteria were isolated. In the experimental animals, the amount of endotoxin in the periapical tissues increased gradually from 1 to 70 days, and its level was significantly greater than that of control animals after 7 days. Gram-negative bacteria were isolated from the periapical tissues and their number gradually increased from 1 to 14 days (26 to 82%), but decreased at 21 days. It was approximately 60% from 28 to 70 days. The results of this study showed that the amount of endotoxin in the periapical tissues gradually increased with increasing time and that Gram-negative bacteria were isolated from the same region but did not increase in number concurrently with the increase in the amount of endotoxin.
Subject(s)
Endotoxins/analysis , Periapical Periodontitis/microbiology , Animals , Gram-Negative Bacteria/isolation & purification , Male , Rats , Rats, WistarABSTRACT
Pulmonary sequestration is uncommon. We report a case with radiographically visible calcification and elevated serum values of the tumor markers carcinoembryonic antigen (CEA) and carbohydrate antigen (CA) 19-9.
Subject(s)
Antigens, Tumor-Associated, Carbohydrate/blood , Bronchopulmonary Sequestration/diagnosis , Carcinoembryonic Antigen/blood , Adult , Biomarkers, Tumor , Female , Humans , Tomography, X-Ray ComputedABSTRACT
A case of a 47-year-old woman with pulmonary varix is reported herein. Saccular dilatation of the inferior pulmonary vein resembled a pulmonary perihilar mass which could not be palpated at the time of thoracotomy. Aneurysmal dilatation of the pulmonary vein, otherwise known as pulmonary varix, is rare. Only 71 such cases, including 17 cases in Japan, have been reported. Pulmonary varices may be classified into three types, namely: saccular type, tortuous type and confluent type. Most of the varices seen in patients with valvular disease have been of the confluent type (62 per cent), however tortuous type varices have also been seen in some cases (19 per cent). Pulmonary venous hypertension may be one of the major causes of confluent type pulmonary varices as regression of pulmonary varices after mitral valve replacement has been reported. None of the saccular type cases, however, were accompanied by valvular disease. This indicates that local factors may also be an important cause of saccular type varices.
