ABSTRACT
The carbonization of wool fleece (WF) is conducted to remove the adhered vegetable matter (VM) from contaminated WF using sulfuric acid, followed by drying and backing. This process has a deteriorative effect on WF and requires a tremendous quantity of water for rinsing WF after carbonization to remove any H2SO4 residuals. Herein, we propose an alternative eco-friendly water-saving process for the removal of VM from WF using enzymes. Cellulase-containing xylanase from the fungus Aspergillus terreus, and cellulase-free xylanase from the fungus Aspergillus flavus AW1 were used to remove the VM from WF. The effect of some process parameters on the amount of the removed VM was assessed. Alkali solubility as well as sulfur and cystine content were used to follow the alteration in the chemistry of the bio-treated WF. The fiber morphology was examined using scanning electron microscopy. The dyeability of the treated WF towards acid, reactive, and basic dyes was monitored. The results revealed that the removal of the VM from WF by applying the examined enzymes was effective and could be an appropriate, non-destructive, eco-friendly water-saving substitute to the conventional carbonization procedures. By virtue of enzyme specificity, the proposed process removed the VM without deteriorating the fiber.
Subject(s)
Cellulase , Animals , Vegetables , Wool , Water , CarbohydratesABSTRACT
A new rapid, simple, and sensitive RP-HPLC method was carried out through applying Quality by Design approach for determination of xipamide and valsartan in Human plasma. Fractional factorial design was used for screening of four independent factors: pH, flow rate, detection wavelength, and % of MeOH. Analysis of variance (ANOVA) confirmed that flow rate and % of MeOH were only significant. Chromatographic conditions optimization was carried out through using central composite design. Method analysis was performed using BDS Hypersil C8 column (250 × 4.6 mm, 5 µm) and an isocratic mobile phase of MeOH and 0.05 M KH2PO4 buffer pH 3 (64.5:35.5, v/v) at 1.2 mL/min flow rate with UV detection at 240 nm and 10 µL injection volume. According to FDA guidelines, the method was then validated for the determination of the two drugs clinically in human plasma in respect of future pharmacokinetic and bioequivalence simulation studies. The standard curve was linear in the concentration range of 5-100 µg/mL for both drugs, with a determination coefficient (R2) of 0.999. Also, the average recoveries lied within the range from 99.89 to 100.03%. The proposed method showed good predictability and robustness.
ABSTRACT
ABSTRACT Response surface methodology was used to optimize lincomycin production by Streptomyces lincolnensis NRRL ISP-5355 in submerged fermentation. Screening of fermentation medium components to find their relative effect on lincomycin production was done using Plackett-Burman design. Malt extract, dextrin, soluble starch and (NH4)2SO4 were the most significant nutrient influenced on lincomycin production. Central composite design was applied to determine optimal concentrations of these factors and the effect of their mutual interactions. The interaction between soluble starch and (NH4)2SO4 was found to enhance the production, whereas malt extract and dextrin exhibited an influence independent from the other two factors. Using this statistical optimization method, maximum lincomycin concentration of 1345 μg/ml was obtained which represented a 40.5 % increase in titer than that acquired from the non-optimized medium. This statistically optimized medium was employed for lincomycin production through immobilization of Streptomyces lincolnensis by adsorption on synthetic cotton fibers. Immobilization technique improved the concentration to 1350 μg/ml higher than that produced from free cells cultures and could be maintained for longer than 17 days in a repeated batch system.
