ABSTRACT
UNLABELLED: Forty adult female rats were randomly divided into four groups: control, nicotine, nicotine+vitamin C and nicotine+selenium group. Splenic tissues concentrations of thiobarbituric acid reactive substances (TBARS), nitric oxide, superoxide dismutase (SOD) and catalase (CAT) activities were measured. The P53 and Bcl2 proteins were detected by Western blot and their expression in splenic tissues were measured by quantitative real time PCR in all groups. Compared to control group, nicotine increased the concentrations of TBARS and nitric oxide significantly. However, Vit. C or Se supplementation with nicotine caused a significant decrease in these concentrations. SOD and CAT activities of nicotine group decreased significantly compared to control group. Treatment with Vit. C or Se plays a significant role in elevation of SOD and CAT activities. In splenic tissues, nicotine significantly decreases the protein levels and the mRNA expression of P53 and increases the protein levels of Bcl2 and its expression. Administration of Vit. C. to nicotine-treated rats completely reversed the decrease in P53 levels and its mRNA expression and the increase in Bcl2 levels and its mRNA expression to the control values. In contrast, Se administration did not induce any significant changes in these genes levels or expressions compared to nicotine group. CONCLUSION: Vit. C supplementation to nicotine treated rats was more effective than selenium in attenuation of nicotine-induced oxidative stress, p53 and Bcl2 expression in rat spleen tissues.
ABSTRACT
BACKGROUND: The aim of this study was to assess the dental health status of Saudi schoolboys in an urban city in the southwestern region of Saudi Arabia, and to investigate the association of dental caries with wasting and stunted growth. PATIENTS AND METHODS: A two-stage stratified random sample of 959 schoolboys representative of the 6 to 13 year-old school population in Abha were subjected to dental examination. Using the WHO criteria, decayed, missing and filled teeth were determined, and the prevalence of caries for each age group was determined by means of decayed and filled primary teeth (dft), and decayed, missing and filled permanent teeth (DMFT) epidemiological indices. The children were also subjected to weight and height measurements and their values were compared with the local growth standards. RESULTS: The total prevalence of caries-free children was 14.6%. The mean dft values at ages 6, 9 and 12 years were 6.53, 3.97 and 1.07, respectively (P<0.001), while the mean DMFT values were 0.16, 0.83 and 1.23 for such ages (P<0.001). After adjusting for age and social class, the wasted children were found to have a higher caries prevalence in the primary dentition than the well-nourished children (P<0.01), while children with stunted growth exhibited lower caries prevalence in the permanent teeth (P<0.05). CONCLUSION: Comparisons with international goals for oral health by the year 2000 show that the goal for 12 year-olds with respect to the status of the permanent dentition has been attained, but not the goal for 5-6 year olds with respect to caries-free primary teeth. It is likely that nutritional status may have different effects on susceptibility to caries of deciduous and permanent teeth.
