Effects of single-dose and co-supplementation of vitamin D and omega-3 on metabolic profile in women with polycystic ovary syndrome: An RCT.

Background: Polycystic ovary syndrome (PCOS) is a heterogeneous medical condition with a cluster of metabolic and endocrine disorders including dyslipidemia, insulin resistance, and hyperandrogenism. Objective: The present study aimed to determine the effects of single-dose and co-supplementation of vitamin D (vit D) and omega-3 (O3) on anthropometric and several biochemical factors in women with PCOS. Materials and Methods: In this double-blind, randomized clinical trial, 80 PCOS women referred to Shahid Motahhari Clinic, Shiraz, Iran, from April to October 2017 were studied in 4 groups (n = 20/each) for 8 wk. The placebo group received the placebo capsule (paraffin oil); 1 weekly and 2/daily; the vit D group received vit D (50,000 IU/weekly) + 2 placebo capsules daily, O3 group, 2, O3 capsules daily + 1 placebo capsule weekly, and vit D + O3 (50000 IU/weekly vit D + 2, O3 capsules daily). Before and after 8 wk of intervention, height, weight, body mass index, waist circumference, triglycerides, total cholesterol (TC), high-density lipoprotein cholesterol, low-density lipoprotein cholesterol, fasting blood sugar, homeostasis model of insulin resistance index, and sex hormone binding globulin were compared between groups. Results: The significant reduction was detected in serum triglyceride (p = 0.002), TC (p = 0.04), fasting blood sugar (p = 0.02), insulin (p = 0.001), and homeostasis model of insulin resistance index (p = 0.001) concentrations in all vit D, O3, and vit D + O3 supplemented groups compared to the placebo group. Furthermore, in comparison with the placebo group, a significant increase was observed in serum sex hormone binding globulin levels after vit D, O3, and vit D + O3 treatments. Nevertheless, no significant changes were observed in serum high-density lipoprotein cholesterol, low-density lipoprotein cholesterol, and anthropometric indices in all treated participants. Conclusion: The current study indicated that single dose and co-supplementation of vit D and O3 for 8 wk was associated with beneficial effects on serum triglyceride, TC, insulin, and sex hormone binding globulin concentrations among women suffering from PCOS.

High dietary inflammatory index (DII) scores increase odds of overweight in adults with rs9939609 polymorphism of FTO gene.

BACKGROUND: Obesity/overweight is a public health problem globally. Dietary induced inflammation is among the most critical risk factors modulating overweight/obesity. Some people genetically are at higher risk for obesity/overweight. The first gene contributing to conventional forms of human obesity is the FTO gene. The associations between genes like the FTO, inflammation, and obesity/overweight have been investigated in limited studies. We aimed to investigate the association between the dietary inflammatory index (DII) and odds of obesity/overweight in adults with rs9939609 polymorphism of the FTO gene. METHODS: A valid 168-item semi-quantitative food frequency questionnaire was used to assess dietary intake. To investigate the role of diet in the development of inflammation, we used the DII, which is predictive of serum inflammatory markers' levels. The Inclusion criteria were defined as body mass index (BMI) from 24.9 to 29.9 kg/m2, age from 20 to 45 years, not participating in a weight management program during two past months, and no weight loss greater than 5%. We determined the genotypes of FTO rs9939609 polymorphism via amplification refractory mutation system polymerase chain reaction (ARMS-PCR) in a retrospective chart review. RESULTS: Results obtained from modeling the DII as a continuous variable and odds of obesity/overweight showed a significant association after multivariate adjustment for sex, height, fat mass, systolic blood pressure (SBP), and total energy intake (OR = 2.83 CI = 1.16-6.91). In addition, logistic regression models with the DII as a dichotomous variable adjusting for sex, height, fat mass, SBP, and total energy intake showed subjects with the DII score<0.49 were at 2.5 times higher odds of having overweight compared to subjects with the DII ≥0.49 (ORDII<0.49/≥0.49 = 2.44 CI = 1.12-5.32). CONCLUSION: Dietary induced inflammation significantly is related to odds of overweight in adults with rs9939609 polymorphism of the FTO gene. Suggesting an anti-inflammatory diet containing vitamins and minerals such as vitamin A, thiamine, niacin, and zinc, manganese, and selenium and recommending a reduction in the most inflammatory factors of diet, including saturated and trans fatty acids, could be a new strategy in the treatment and or controlling of obesity/overweight as a public health problem.

Effect of conjugated linoleic acid and omega-3 fatty acid supplementation on inflammatory and oxidative stress markers in atherosclerotic patients.

BACKGROUND: Cardiovascular disease is the major cause of morbidity, mortality, and disability in Iranian people. Inflammation and oxidative processes are key components of cardiovascular disease. The aim of this study was to evaluate the effect of conjugated linoleic acids (CLA) and omega-3 fatty acid (ω-3 fatty acids) supplementation on inflammation markers and oxidative stress in atherosclerotic patients. METHODS: This study was a two-month clinical, randomized trial. 90 volunteers who referred to Emam Reza Heart Clinic of Shiraz University of Medical Sciences (Shiraz, Iran) from February to March 2011 and had the inclusion criteria of this study were selected. Participants were classified into 3 groups receiving 3 g/d CLA, 1920 mg/d ω-3, or placebo for 2 months. C-reactive protein (CRP), interleukin-6 (IL-6), malondialdehyde (MDA), and glutathione peroxidase (GPx) were measured before and after supplementation. RESULTS: The hs-CRP level decreased significantly in both the omega-3 and CLA group (P < 0.05). IL-6 reduced significantly in the ω-3 group, but the reduction of IL-6 levels in the CLA group was not significant. GPx increased in the CLA and omega-3 groups (P < 0.05). MDA level decreased significantly in both omega-3 and CLA groups (P < 0.05). Comparison between the groups indicates a significant change in CRP levels in the ω-3 group relative to the control group. However, other indices did not cause any significant change in the ω-3 and CLA groups in comparison to the control group. CONCLUSION: Diet supplementation with CLA and ω-3 can have a beneficial effect on some indices of inflammatory and oxidative stress.

A comparative study of selenium concentration and glutathione peroxidase activity in normal and breast cancer patients.

OBJECTIVE: The present study was undertaken to compare plasma Se values and glutathione peroxidase (GPX) activity in normal and breast cancer patients. DESIGN: In a case-control study, forty-five breast cancer patients and the same number of healthy women were randomly selected from their population. Se was measured in plasma by atomic absorption spectrophotometry and GPX activity in erythrocytes was measured using a standard spectrophotometric method. RESULTS: Plasma Se concentration in healthy women and breast cancer patients was in the normal range, with no statistically significant difference observed between the two groups (138.40 (sd 40.36) microg/l v. 132.15 (sd 35.37) microg/l, respectively). Erythrocyte GPX activity was significantly (P<0.01) higher in breast cancer patients (24.81 (sd 11.66) U/g Hb) compared with healthy women (20.29 (sd 4.24) U/g Hb). CONCLUSION: The present study indicated that Se deficiency was not a problem in the participants, and sufficient quantity of this element could increase GPX activity to have a protective effect against oxidative damage.