Impacts of free nitrous acid on stabilizing food waste and sewage sludge for anaerobic digestion.

This work investigated the effectiveness of free nitrous acid (FNA) in enhancing organic waste solubilization to improve biogas production in anaerobic digestion (AD). The results indicated that FNA pretreatment can enhance soluble organic content and control H2S odor in tested organic wastes, including food waste, sewage sludge, and their combination. However, a significant decrease (>50 %) in FNA concentration was found in the reactors, possibly due to denitrifier-driven NO2- consumption. Biochemical methane potential (BMP) tests showed a 25 ± 8 % enhancement in CH4 production in the reactors fed with mixed substrate pretreated with 2.9 mg FNA-N/L. However, the presence of NO2- (325.6-2368.0 mg N/L) in some BMP reactors, due to carryover from FNA pretreatment, adversely affected CH4 production (>55 %) and prolonged lag time (>4.2 times). These findings are valuable for researchers and practitioners in waste management, offering insights for implementing FNA pretreatment to enhance the biodegradability of organic wastes in AD.

Removal performance and inhibitory effects of combined tetracycline, oxytetracycline, sulfadiazine, and norfloxacin on anaerobic digestion process treating swine manure.

Combined veterinary antibiotics (CVAs) belonging to different antibiotics classes could cause exacerbated impacts on the anaerobic digestion (AD) process of swine manure. Four different antibiotics "two tetracyclines: tetracycline (TC) and oxytetracycline (OTC), one fluoroquinolones: norfloxacin (Norf), and one sulfonamides: sulfadiazine (SDZ)" were combined to evaluate their removal performances and its inhibition effects on AD. Results indicated that CVAs removal decreased from 84.3 to 63.7 %, with an increase in the initial concentration from 12.5 to 50 mg L-1, where the removal of CVAs occurring in the order OTC > TC > Norf > SDZ. An average of 9.5, 7.5, 9.5, and 32.1 % of the spiked TC, OTC, SDZ, and Norf were remained in the sludge, respectively. With 50 mg L-1 of CVAs, a competitive adsorption phenomenon was found to have a notable impact on biodegradation microorganisms' activity leading a 73.1 % decrease in CH4 production. CVAs caused a temporal inhibition to the acidogenic activity followed by partial inhibition to methanogenic by 66.8 %, and IC50 was 38.5 mg L-1. Moreover, CVAs resulted in acetate accumulation, while 26 % and 48 % lower in TS and COD removal, respectively, were observed. A significant reduction in the relative abundance of bacteria and archaeal genera was also mentioned. The findings of this research would provide a more in-depth understanding of AD's performance in treating swine manure contaminated with combined antibiotics.

Impact of electro-conductive nanoparticles additives on anaerobic digestion performance - A review.

Anaerobic digestion (AD) is a biochemical process that converts waste organic matter into energy-rich biogas with methane as the main component. Addition of electric electro-conductive, such as that nanoparticles (NP), has been shown to improve biogas generation. Interspecies electron transfer and direct interspecies electron transfer (DIET) using conductive materials is one of the mechanisms responsible for observed increases in CH4. This article discusses the effect of the type and size of electro-conductive NPs on improving microbial degradation within AD systems, as well as the effect of electro-conductive NPs on microbial community shifts and syntrophic metabolism. Limitations and future perspectives of using NPs in an AD system is also discussed.

Effect of metal nanoparticles in anaerobic digestion production and plant uptake from effluent fertilizer.

Nanoparticle (NP) use can increase biological activity and adversely impact the environment. This study was the first to quantify biogas increases with NP mixtures during continuous anaerobic digestion (AD) of poultry litter and NP uptake in crops through tracking: 1) CH4 and H2S production from a NP mixture (Fe, Ni, and Co) in 30 L continuous digester (AD1) for 278 days compared to a control digester (AD2) without NP addition, 2) NP degradation during digestion, 3) using AD effluent with and without NP addition as a fertilizer, and 4) plant uptake of NPs. With NP inclusion, CH4 production increased by 23.7%, and H2S was reduced by 56.3%. The AD1 effluent had 1,160-19,400% higher NP concentrations and the lettuce biomass had 21.0-1,920% more NPs than lettuce fertilized with the AD2 effluent. This study showed that the effects of NPs remaining in the AD effluent must be considered.

Impact of metal nanoparticles on biogas production from poultry litter.

The effects of metal nanoparticle (NP) addition during anaerobic digestion (AD) of poultry litter was tested using two sequential experiments: Exp. A) four NPs (Fe, Ni, Co, and Fe3O4) at three concentrations; and Exp. B) NP combinations (Fe, Ni, and Co) at four concentrations. Scanning electronic microscopy (SEM) and elemental analysis were used to confirm NP inclusion after dispersion (before AD) and track nanoparticles post-AD, and new technique for NP extraction post-AD was developed. Before AD, NPs ranged from 30.0 to 80.9 nm for Fe, Ni, and Co, and 94.3 to 400 nm for Fe3O4. Methane production increased with NPs addition compared to poultry litter-only, with the highest increases observed with NPs concentrations (in mg/L) of 12 Ni (38.4% increase), 5.4 Co (29.7% increase), 100 Fe (29.1% increase), and 15 Fe3O4 (27.5% increase). Nanoparticle mixtures greatly decreased H2S production. The SEM post-AD detected Fe, Ni, and Fe3O4 at concentrations ≥100 mg/L.