ABSTRACT
A screening study yielded Acinetobacter calcoaceticus strain 4-1-5, which is capable of hydroxylating indole to 7-hydroxyindole. Strain 4-1-5 grew on terephthalate as the sole source of carbon and energy and hydroxylated indole to 7-hydroxyindole by cometabolism of indole using terephthalate as cosubstrate. Strain 4-1-5 produced 0.574 mM of 7-hydroxyindole at 2.38 mM indole in 24 h with the cell growth.
Subject(s)
Acinetobacter calcoaceticus/metabolism , Indoles/chemistry , Indoles/metabolism , Biodegradation, Environmental , HydroxylationABSTRACT
Two microorganisms (NDKK48 and NDKY76A) that degrade long-chain cyclic alkanes (c-alkanes) were isolated from soil samples. Strains NDKK48 and NDKY76A were identified as Rhodococcus sp. and Gordonia sp., respectively. Both strains used not only normal alkane (n-alkane) but also c-alkane as a sole carbon and energy source, and the strains degraded more than 27% of car engine base oil (1% addition).
Subject(s)
Automobiles , Gordonia Bacterium/classification , Gordonia Bacterium/metabolism , Petroleum/metabolism , Rhodococcus/classification , Rhodococcus/metabolism , Alkanes/metabolism , Biodegradation, Environmental , Soil MicrobiologyABSTRACT
The degradation of alkylcyclohexane by Acinetobacter sp. ODDK71 was investigated. Strain ODDK71 degraded alkylcyclohexanes (alkyl side chain length of > or = 12) by co-metabolism when hexadecane was used as a growth substrate. GGMS analysis of co-metabolized products from dodecylcyclohexane suggests that strain ODDK71 degraded dodecylcyclohexane via a ring oxidation and an alkyl side chain oxidation pathways. The ring oxidation pathway of dodecylcyclohexane is a novel pathway of microbial degradation of dodecylcyclohexane.