Layer 5 Intratelencephalic Neurons in the Motor Cortex Stably Encode Skilled Movement.

The primary motor cortex (M1) and the dorsal striatum play a critical role in motor learning and the retention of learned behaviors. Motor representations of corticostriatal ensembles emerge during motor learning. In the coordinated reorganization of M1 and the dorsal striatum for motor learning, layer 5a (L5a) which connects M1 to the ipsilateral and contralateral dorsal striatum, should be a key layer. Although M1 L5a neurons represent movement-related activity in the late stage of learning, it is unclear whether the activity is retained as a memory engram. Here, using Tlx3-Cre male transgenic mice, we conducted two-photon calcium imaging of striatum-projecting L5a intratelencephalic (IT) neurons in forelimb M1 during late sessions of a self-initiated lever-pull task and in sessions after 6 d of nontraining following the late sessions. We found that trained male animals exhibited stable motor performance before and after the nontraining days. At the same time, we found that M1 L5a IT neurons strongly represented the well-learned forelimb movement but not uninstructed orofacial movements. A subset of M1 L5a IT neurons consistently coded the well-learned forelimb movement before and after the nontraining days. Inactivation of M1 IT neurons after learning impaired task performance when the lever was made heavier or when the target range of the pull distance was narrowed. These results suggest that a subset of M1 L5a IT neurons continuously represent skilled movement after learning and serve to fine-tune the kinematics of well-learned movement.SIGNIFICANCE STATEMENT Motor memory persists even when it is not used for a while. IT neurons in L5a of the M1 gradually come to represent skilled forelimb movements during motor learning. However, it remains to be determined whether these changes persist over a long period and how these neurons contribute to skilled movements. Here, we show that a subset of M1 L5a IT neurons retain information for skilled forelimb movements even after nontraining days. Furthermore, suppressing the activity of these neurons during skilled forelimb movements impaired behavioral stability and adaptability. Our results suggest the importance of M1 L5a IT neurons for tuning skilled forelimb movements over a long period.

Serotonin induces depolarization in lateral amygdala neurons by activation of TRPC-like current and inhibition of GIRK current depending on 5-HT(2C) receptor.

Regional differences are known in the serotonin-induced modulation of neuronal activity within the amygdala. This in vitro study in rats focuses on analyzing the ionic mechanism underlying serotonin-induced depolarization in the lateral amygdala. Serotonin depolarized membrane potential by 5 mV, which is underlain by a serotonin-induced inward current at rest with a characteristic reversal potential of -105 mV. From pharmacological experiments, the 5-HT2C subtype was singled out as the receptor subtype involved. Under blockade of K(+) channels by Ba(2+), 5-HT induced an inward current with no reversal at the range between -50 and -130 mV, which was identified as a TRPC-like current. This current was blocked by the specific phosphatidylinositol 3-kinse (PI3-kinase) inhibitor LY294002, pointing to its dependence on PI3-kinase. The Ba(2+)-sensitive component, obtained by subtraction, showed a strong outward rectification and the reversal potential of K(+), indicating that this component results from a serotonin-induced inhibition of G-protein coupled inwardly rectifying K(+) channel (GIRK) current. By wortmannin, an inhibitor of both PI3-kinase and PI4-kinase, a serotonin-induced phosphatidylinositol 4,5-bisphosphate (PIP2) depletion was revealed to underlie GIRK inhibition. Thus, the serotonin-induced current turned out to be caused by a combined occurrence of GIRK inhibition and PI3-kinase-dependent TRPC-like current. With serotonergic modulation, all these mechanisms should be recruited in lateral amygdala principal neurons and likely contribute to generation of region-specific neuronal activity patterns within the amygdala, which may at least partly implement its required role in fear and anxiety.