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Fish Shellfish Immunol ; 87: 87-95, 2019 Apr.
Article in English | MEDLINE | ID: mdl-30615987

ABSTRACT

Edwardsiella ictaluri is a causative agent of enteric septicemia of catfish (ESC), a seriously lethal disease in Vietnamese catfish (Pangasius hypophthalmus). A safe and effective vaccine against ESC is currently an urgent demand due to antibiotic overuse in pangasius farms has led to an alarming antimicrobial resistance. In this study, two E. ictaluri wzzE mutants (WzM-L3, deficient in a 1038bp-entire wzzE gene and WzM-S3, a 245bp-partial deletion of wzzE) were developed and their protection efficiacy was evaluated in hatched pangasius against ESC by immersion vaccination. As comparing to the high virulent wild-type strain who caused 73.33% of death on pangasius fingerlings immersed at 7.1 × 106 CFU ml-1, both mutants showed extremely low mortality rates at 3.33% (WzM-S3) and 0% (WzM-L3) on pangasius fingerlings immersed at high concentration of 1.5 × 107 CFU mL-1 and 9.7 × 106 CFU ml-1, respectively. Interestingly, both WzM-S3 and WzM-L3 had a remarkably high protection against ESC, as RPS % were found at 89.29% and 90%, respectively. The mutant WzM-L3 is a potential live attenuated vaccine against ESC in Vietnamese catfish farms with good protection and simple practice.


Subject(s)
Bacterial Vaccines/immunology , Catfishes/immunology , Edwardsiella ictaluri/immunology , Enterobacteriaceae Infections/veterinary , Fish Diseases/prevention & control , Animals , Bacterial Vaccines/genetics , Catfishes/microbiology , Edwardsiella ictaluri/genetics , Enterobacteriaceae Infections/immunology , Enterobacteriaceae Infections/prevention & control , Fish Diseases/immunology , Fish Diseases/microbiology , Gene Deletion , Mutation , Vaccines, Attenuated/genetics , Vaccines, Attenuated/immunology
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