ABSTRACT
Serial 10-micron cryostat cross-sections of the tensor tympani muscle and of the medial gastrocnemius muscle from adult domestic cats were incubated for myofibrillar ATPase, NADH tetrazolium reductase (NADH-TR), succinic dehydrogenase (SDH), malate dehydrogenase (MDH) and menadione-linked alpha-glycerophosphate dehydrogenase (alpha GPDH). The optical density of individual tensor tympani and gastrocnemius muscle fibres after different incubation procedures was measured photometrically. The absorbance values of the tensor tympani fibres were related to the values of the type I, type IIA and type IIB fibres of the gastrocnemius muscle. Only two different types of fibre could be demonstrated in the tensor tympani, one type resembling the type I and another resembling the type IIA of the gastrocnemius muscle. The findings are discussed in relation to other, recent immunohistochemical studies on cat tensor tympani muscle fibres.
Subject(s)
Tensor Tympani/anatomy & histology , Tympanic Membrane/anatomy & histology , Animals , Cats , Histocytochemistry , Photometry , Tensor Tympani/enzymologyABSTRACT
ABR from cats were measured by use of standard EEG surface electrodes and ERA Madsen-74 equipment. A method for recording the waveform of the stimulus at the ear drum is demonstrated. ABR to both phases of three different stimuli was compared. In four out of five cats the ABR latency for rarefaction clicks was shown to be shorter than for condensation clicks at high stimulus levels. This effect of stimulus polarity was most pronounced for stimuli with few and broad peaks in the acoustic waveform. At lower stimulus intensities no differences in ABR latency could be demonstrated by reversing stimulus polarity. It is suggested that the first negative deflection in the acoustic waveform is mainly responsible for the ABR at high stimulus level. At lower stimulus level not only the onset but a greater part of the acoustic signal is responsible for the response.
Subject(s)
Acoustic Stimulation , Brain Stem/physiology , Evoked Potentials, Auditory , Animals , Body Temperature , Cats , Reaction TimeSubject(s)
Bacteria/isolation & purification , Otitis Media, Suppurative/microbiology , Otitis Media/microbiology , Acute Disease , Child , Child, Preschool , Ear, Middle/microbiology , Humans , Infant , Nasopharynx/microbiology , Otitis Media, Suppurative/therapy , Penicillins/therapeutic use , Tympanic Membrane/surgeryABSTRACT
The effect of myringotomy versus Penicillin therapy in the treatment of patients with acute suppurative otitis media was compared. A third group of patients treated with both myringotomy and Penicillin was included in the investigation. Ten days after institution of the treatment there were significantly more days with secretion/bulging drum in the myringotomy group than in the two other groups. At this time Penicillin therapy was started also in those patients belonging to the myringotomy group. With this reservation the occurrence of such late complications as secretory otitis media and relapses was the same, independent of treatment. No cases of mastoiditis were observed.
