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Biomed Opt Express ; 12(1): 320-335, 2021 Jan 01.
Article in English | MEDLINE | ID: mdl-33659078

ABSTRACT

In general, the measurement of the main three optical properties (µ a , µ s and g) in turbid media requires a very precise measurement of the total transmission (TT), the total reflection (TR) and the collimated transmission (CT). Furthermore, an inverse algorithm such as inverse adding doubling or inverse Monte-Carlo-simulations is required for the reconstruction of the optical properties. Despite many available methods, the error free measurement of the scattering coefficient or the g-factor still remains challenging. In this study, we present a way to directly calculate the scattering coefficient from the total and collimated transmission. To allow this, it can be shown that T T C T is proportional to e µ s ⋅ d for a wide range of optical properties if the sample is thick enough. Moreover, a set-up is developed and validated to measure the collimated transmission precisely.

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