ABSTRACT
Our in vitro data indicate that peripheral blood monocytes or monocyte-derived immature dendritic cells under appropriate culture conditions transdifferentiate into endothelial-like cells (ELC), which are characterized by the expression of endothelial markers and the formation of tube-like structures. Dependent on the culture conditions a mixed macrophage/endothelial or an endothelial phenotype could be induced. A similar pattern of development could be seen in CD14+ monocyte-derived ELC and ELC grown from CD34+ precursor cells or from dendritic cells generated from CD34+ cells. These in vitro data suggest that monocytes are precursors of different subgroups of endothelial cells and that the formation of endothelial cells from CD34+ progenitor cells follows a similar pathway possibly via the monocyte and/or the immature dendritic cell.
Subject(s)
Cell Differentiation/physiology , Cell Lineage/physiology , Cells, Cultured/cytology , Dendritic Cells/cytology , Monocytes/cytology , Neovascularization, Physiologic/physiology , Stem Cells/cytology , Animals , Antigens, CD34/immunology , Cells, Cultured/immunology , Cells, Cultured/metabolism , Dendritic Cells/immunology , Dendritic Cells/metabolism , Humans , In Vitro Techniques , Lipopolysaccharide Receptors/immunology , Monocytes/immunology , Monocytes/metabolism , Stem Cells/immunology , Stem Cells/metabolismABSTRACT
PURPOSE: This trial of the German High-Grade Non-Hodgkin's Lymphoma Study Group compares the use of high-dose therapy (HDT) as part of primary treatment with cyclophosphamide, doxorubicin, vincristine, and prednisone (CHOP) plus etoposide followed by involved-field (IF) radiotherapy in a randomized, multicenter, phase III study. PATIENTS AND METHODS: Three hundred twelve patients with "aggressive" non-Hodgkin's lymphoma aged Subject(s)
Antineoplastic Combined Chemotherapy Protocols/therapeutic use
, L-Lactate Dehydrogenase/blood
, Lymphoma, Non-Hodgkin/enzymology
, Lymphoma, Non-Hodgkin/therapy
, Stem Cell Transplantation
, Antineoplastic Combined Chemotherapy Protocols/administration & dosage
, Carmustine/administration & dosage
, Chemotherapy, Adjuvant
, Cyclophosphamide/administration & dosage
, Cytarabine/administration & dosage
, Doxorubicin/administration & dosage
, Drug Administration Schedule
, Etoposide/administration & dosage
, Female
, Germany
, Humans
, Lymphoma, Non-Hodgkin/drug therapy
, Lymphoma, Non-Hodgkin/radiotherapy
, Male
, Melphalan/administration & dosage
, Middle Aged
, Prednisolone/administration & dosage
, Prognosis
, Prospective Studies
, Radiotherapy, Adjuvant
, Risk
, Survival Analysis
, Transplantation, Autologous
, Treatment Outcome
, Vincristine/administration & dosage
