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1.
Acta Crystallogr F Struct Biol Commun ; 79(Pt 1): 17-22, 2023 Jan 01.
Article in English | MEDLINE | ID: mdl-36598352

ABSTRACT

Plant cells form microtubule arrays, called `cortical microtubules', beneath the plasma membrane which are critical for cell-wall organization and directional cell growth. Cortical microtubules are nucleated independently of centrosomes. Spiral2 is a land-plant-specific microtubule minus-end-targeting protein that stabilizes the minus ends by inhibiting depolymerization of the filament. Spiral2 possesses an N-terminal microtubule-binding domain and a conserved C-terminal domain whose function is unknown. In this study, the crystal structure of the conserved C-terminal domain of Spiral2 was determined using the single-wavelength anomalous dispersion method. Refinement of the model to a resolution of 2.2 Šrevealed a helix-turn-helix fold with seven α-helices. The protein crystallized as a dimer, but SEC-MALS analysis showed the protein to be monomeric. A structural homology search revealed that the protein has similarity to the C-terminal domain of the katanin regulatory subunit p80. The structure presented here suggests that the C-terminal domain of Spiral2 represents a new class of microtubule dynamics modulator across the kingdom.


Subject(s)
Microtubule-Associated Proteins , Microtubules , Microtubule-Associated Proteins/genetics , Microtubule-Associated Proteins/metabolism , Crystallography, X-Ray , Microtubules/metabolism , Katanin/chemistry , Katanin/metabolism , Plant Proteins/genetics , Plant Proteins/metabolism
2.
J Biol Chem ; 295(51): 17770-17780, 2020 12 18.
Article in English | MEDLINE | ID: mdl-33454013

ABSTRACT

Bacterial low-copy-number plasmids require partition (par) systems to ensure their stable inheritance by daughter cells. In general, these systems consist of three components: a centromeric DNA sequence, a centromere-binding protein and a nucleotide hydrolase that polymerizes and functions as a motor. Type III systems, however, segregate plasmids using three proteins: the FtsZ/tubulin-like GTPase TubZ, the centromere-binding protein TubR and the MerR-like transcriptional regulator TubY. Although the TubZ filament is sufficient to transport the TubR-centromere complex in vitro, TubY is still necessary for the stable maintenance of the plasmid. TubY contains an N-terminal DNA-binding helix-turn-helix motif and a C-terminal coiled-coil followed by a cluster of lysine residues. This study determined the crystal structure of the C-terminal domain of TubY from the Bacillus cereus pXO1-like plasmid and showed that it forms a tetrameric parallel four-helix bundle that differs from the typical MerR family proteins with a dimeric anti-parallel coiled-coil. Biochemical analyses revealed that the C-terminal tail with the conserved lysine cluster helps TubY to stably associate with the TubR-centromere complex as well as to nonspecifically bind DNA. Furthermore, this C-terminal tail forms an amphipathic helix in the presence of lipids but must oligomerize to localize the protein to the membrane in vivo. Taken together, these data suggest that TubY is a component of the nucleoprotein complex within the partitioning machinery, and that lipid membranes act as mediators of type III systems.


Subject(s)
Bacterial Proteins/metabolism , DNA/metabolism , Amino Acid Sequence , Bacillus cereus/metabolism , Bacterial Proteins/chemistry , Cell Membrane/chemistry , Cell Membrane/metabolism , Centromere/metabolism , Crystallography, X-Ray , DNA/chemistry , Helix-Turn-Helix Motifs , Models, Molecular , Phospholipids/chemistry , Phospholipids/metabolism , Plasmids/metabolism , Protein Binding , Protein Domains , Protein Multimerization , Protein Structure, Quaternary , Sequence Alignment
3.
J Cell Biol ; 219(2)2020 02 03.
Article in English | MEDLINE | ID: mdl-31757788

ABSTRACT

CLASPs are conserved microtubule plus-end-tracking proteins that suppress microtubule catastrophes and independently localize to kinetochores during mitosis. Thus, CLASPs are ideally positioned to regulate kinetochore-microtubule dynamics required for chromosome segregation fidelity, but the underlying mechanism remains unknown. Here, we found that human CLASP2 exists predominantly as a monomer in solution, but it can self-associate through its C-terminal kinetochore-binding domain. Kinetochore localization was independent of self-association, and driving monomeric CLASP2 to kinetochores fully rescued normal kinetochore-microtubule dynamics, while partially sustaining mitosis. CLASP2 kinetochore localization, recognition of growing microtubule plus-ends through EB-protein interaction, and the ability to associate with curved microtubule protofilaments through TOG2 and TOG3 domains independently sustained normal spindle length, timely spindle assembly checkpoint satisfaction, chromosome congression, and faithful segregation. Measurements of kinetochore-microtubule half-life and poleward flux revealed that CLASP2 regulates kinetochore-microtubule dynamics by integrating distinctive microtubule-binding properties at the kinetochore-microtubule interface. We propose that kinetochore CLASP2 suppresses microtubule depolymerization and detachment by binding to curved protofilaments at microtubule plus-ends.


Subject(s)
Kinetochores/metabolism , M Phase Cell Cycle Checkpoints/genetics , Microtubule-Associated Proteins/genetics , Chromosome Segregation/genetics , HeLa Cells , Humans , Microtubules/genetics , Mitosis/genetics , Protein Binding/genetics , Protein Domains , Spindle Apparatus/genetics
4.
J Mol Biol ; 430(24): 5015-5028, 2018 12 07.
Article in English | MEDLINE | ID: mdl-30414406

ABSTRACT

Tubulin/FtsZ-like GTPase TubZ is responsible for maintaining the stability of pXO1-like plasmids in virulent Bacilli. TubZ forms a filament in a GTP-dependent manner, and like other partitioning systems of low-copy-number plasmids, it requires the centromere-binding protein TubR that connects the plasmid to the TubZ filament. Systems regulating TubZ partitioning have been identified in Clostridium prophages as well as virulent Bacillus species, in which TubZ facilitates partitioning by binding and towing the segrosome: the nucleoprotein complex composed of TubR and the centromere. However, the molecular mechanisms of segrosome assembly and the transient on-off interactions between the segrosome and the TubZ filament remain poorly understood. Here, we determined the crystal structure of TubR from Bacillus cereus at 2.0-Å resolution and investigated the DNA-binding ability of TubR using hydroxyl radical footprinting and electrophoretic mobility shift assays. The TubR dimer possesses 2-fold symmetry and binds to a 15-bp palindromic consensus sequence in the tubRZ promoter region. Continuous TubR-binding sites overlap each other, which enables efficient binding of TubR in a cooperative manner. Interestingly, the segrosome adopts an extended DNA-protein filament structure and likely gains conformational flexibility by introducing non-consensus residues into the palindromes in an asymmetric manner. Together, our experimental results and structural model indicate that the unique centromere recognition mechanism of TubR allows transient complex formation between the segrosome and the dynamic polymer of TubZ.


Subject(s)
Bacillus cereus/metabolism , Bacterial Proteins/chemistry , Bacterial Proteins/metabolism , Centromere/metabolism , Bacillus cereus/genetics , Bacterial Proteins/genetics , Binding Sites , Circular Dichroism , Crystallography, X-Ray , GTP Phosphohydrolases/chemistry , GTP Phosphohydrolases/metabolism , Models, Molecular , Molecular Dynamics Simulation , Plasmids/genetics , Plasmids/metabolism , Promoter Regions, Genetic
5.
PLoS One ; 12(8): e0182641, 2017.
Article in English | MEDLINE | ID: mdl-28787032

ABSTRACT

The variety of microtubule arrays observed across different cell types should require a diverse group of proteins that control microtubule organization. Nevertheless, mainly because of the intrinsic propensity of microtubules to easily form bundles upon stabilization, only a small number of microtubule crosslinking proteins have been identified, especially in postmitotic cells. Among them is microtubule crosslinking factor 1 (MTCL1) that not only interconnects microtubules via its N-terminal microtubule-binding domain (N-MTBD), but also stabilizes microtubules via its C-terminal microtubule-binding domain (C-MTBD). Here, we comprehensively analyzed the assembly structure of MTCL1 to elucidate the molecular basis of this dual activity in microtubule regulation. Our results indicate that MTCL1 forms a parallel dimer not only through multiple homo-interactions of the central coiled-coil motifs, but also the most C-terminal non-coiled-coil region immediately downstream of the C-MTBD. Among these homo-interaction regions, the first coiled-coil motif adjacent to N-MTBD is sufficient for the MTCL1 function to crosslink microtubules without affecting the dynamic property, and disruption of this motif drastically transformed MTCL1-induced microtubule assembly from tight to network-like bundles. Notably, suppression of the homo-interaction of this motif inhibited the endogenous MTCL1 function to stabilize Golgi-associated microtubules that are essential for Golgi-ribbon formation. Because the microtubule-stabilizing activity of MTCL1 is completely attributed to C-MTBD, the present study suggests possible interplay between N-MTBD and C-MTBD, in which normal crosslinking and accumulation of microtubules by N-MTBD is essential for microtubule stabilization by C-MTBD.


Subject(s)
Microtubule-Associated Proteins/metabolism , Microtubules/metabolism , HEK293 Cells , HeLa Cells , Humans , Microtubule-Associated Proteins/chemistry , Protein Domains , Protein Multimerization , Protein Stability , Protein Structure, Quaternary
6.
J Mol Biol ; 427(14): 2379-95, 2015 Jul 17.
Article in English | MEDLINE | ID: mdl-26003921

ABSTRACT

CLIP-associated proteins CLASPs are mammalian microtubule (MT) plus-end tracking proteins (+TIPs) that promote MT rescue in vivo. Their plus-end localization is dependent on other +TIPs, EB1 and CLIP-170, but in the leading edge of the cell, CLASPs display lattice-binding activity. MT association of CLASPs is suggested to be regulated by multiple TOG (tumor overexpressed gene) domains and by the serine-arginine (SR)-rich region, which contains binding sites for EB1. Here, we report the crystal structures of the two TOG domains of CLASP2. Both domains consist of six HEAT repeats, which are similar to the canonical paddle-like tubulin-binding TOG domains, but have arched conformations. The degrees and directions of curvature are different between the two TOG domains, implying that they have distinct roles in MT binding. Using biochemical, molecular modeling and cell biological analyses, we have investigated the interactions between the TOG domains and αß-tubulin and found that each domain associates differently with αß-tubulin. Our findings suggest that, by varying the degrees of domain curvature, the TOG domains may distinguish the structural conformation of the tubulin dimer, discriminate between different states of MT dynamic instability and thereby function differentially as stabilizers of MTs.


Subject(s)
Microtubule-Associated Proteins/chemistry , Microtubules/metabolism , Amino Acid Sequence , Animals , Binding Sites , Cells, Cultured , Crystallography, X-Ray , HEK293 Cells , Humans , Mice , Microtubule-Associated Proteins/metabolism , Models, Molecular , Molecular Sequence Data , Protein Binding , Protein Multimerization , Protein Structure, Secondary , Protein Structure, Tertiary/physiology
7.
Nat Commun ; 5: 5266, 2014 Nov 04.
Article in English | MEDLINE | ID: mdl-25366663

ABSTRACT

Recent studies have revealed the presence of a microtubule subpopulation called Golgi-derived microtubules that support Golgi ribbon formation, which is required for maintaining polarized cell migration. CLASPs and AKAP450/CG-NAP are involved in their formation, but the underlying molecular mechanisms remain unclear. Here, we find that the microtubule-crosslinking protein, MTCL1, is recruited to the Golgi membranes through interactions with CLASPs and AKAP450/CG-NAP, and promotes microtubule growth from the Golgi membrane. Correspondingly, MTCL1 knockdown specifically impairs the formation of the stable perinuclear microtubule network to which the Golgi ribbon tethers and extends. Rescue experiments demonstrate that besides its crosslinking activity mediated by the N-terminal microtubule-binding region, the C-terminal microtubule-binding region plays essential roles in these MTCL1 functions through a novel microtubule-stabilizing activity. These results suggest that MTCL1 cooperates with CLASPs and AKAP450/CG-NAP in the formation of the Golgi-derived microtubules, and mediates their development into a stable microtubule network.


Subject(s)
Golgi Apparatus/metabolism , Intracellular Membranes/metabolism , Microtubule-Associated Proteins/metabolism , Microtubules/metabolism , A Kinase Anchor Proteins/metabolism , Animals , Cytoskeletal Proteins/metabolism , Gene Knockdown Techniques , HEK293 Cells , HeLa Cells , Humans , Mice , Microtubule-Associated Proteins/chemistry , Microtubule-Associated Proteins/genetics , Rabbits , Rats
8.
Dev Cell ; 30(3): 343-52, 2014 Aug 11.
Article in English | MEDLINE | ID: mdl-25117684

ABSTRACT

Microtubule (MT) plus-end tracking proteins (+TIPs) preferentially localize to MT plus ends. End-binding proteins (EBs) are master regulators of the +TIP complex; however, it is unknown whether EBs are regulated by other +TIPs. Here, we show that cytoplasmic linker-associated proteins (CLASPs) modulate EB localization at MTs. In CLASP-depleted cells, EBs localized along the MT lattice in addition to plus ends. The MT-binding region of CLASP was sufficient for restoring normal EB localization, whereas neither EB-CLASP interactions nor EB tail-binding proteins are involved. In vitro assays revealed that CLASP directly functions to remove EB from MTs. Importantly, this effect occurs specifically during MT polymerization, but not at preformed MTs. Increased GTP-tubulin content within MTs in CLASP-depleted cells suggests that CLASPs facilitate GTP hydrolysis to reduce EB lattice binding. Together, these findings suggest that CLASPs influence the MT lattice itself to regulate EB and determine exclusive plus-end localization of EBs in cells.


Subject(s)
Carrier Proteins/metabolism , Cytoplasm/metabolism , Microtubule-Associated Proteins/metabolism , Microtubules/metabolism , Tubulin/metabolism , Animals , Cell Line , Humans , Protein Binding , Rats
9.
J Cell Sci ; 126(Pt 20): 4671-83, 2013 Oct 15.
Article in English | MEDLINE | ID: mdl-23902687

ABSTRACT

The establishment of epithelial polarity is tightly linked to the dramatic reorganization of microtubules (MTs) from a radial array to a vertical alignment of non-centrosomal MT bundles along the lateral membrane, and a meshwork under the apical and basal membranes. However, little is known about the underlying molecular mechanism of this polarity-dependent MT remodeling. The evolutionarily conserved cell polarity-regulating kinase PAR-1 (known as MARK in mammals), whose activity is essential for maintaining the dynamic state of MTs, has indispensable roles in promoting this process. Here, we identify a novel PAR-1-binding protein, which we call microtubule crosslinking factor 1 (MTCL1), that crosslinks MTs through its N-terminal MT-binding region and subsequent coiled-coil motifs. MTCL1 colocalized with the apicobasal MT bundles in epithelial cells, and its knockdown impaired the development of these MT bundles and the epithelial-cell-specific columnar shape. Rescue experiments revealed that the N-terminal MT-binding region was indispensable for restoring these defects of the knockdown cells. MT regrowth assays indicated that MTCL1 was not required for the initial radial growth of MTs from the apical centrosome but was essential for the accumulation of non-centrosomal MTs to the sublateral regions. Interestingly, MTCL1 recruited a subpopulation of PAR-1b (known as MARK2 in mammals) to the apicobasal MT bundles, and its interaction with PAR-1b was required for MTCL1-dependent development of the apicobasal MT bundles. These results suggest that MTCL1 mediates the epithelial-cell-specific reorganization of non-centrosomal MTs through its MT-crosslinking activity, and cooperates with PAR-1b to maintain the correct temporal balance between dynamic and stable MTs within the apicobasal MT bundles.


Subject(s)
Epithelial Cells/cytology , Epithelial Cells/metabolism , Microtubule-Associated Proteins/metabolism , Microtubules/metabolism , Protein Serine-Threonine Kinases/metabolism , Animals , Cell Polarity/physiology , Cells, Cultured , Dogs , Epithelial Cells/enzymology , HEK293 Cells , HeLa Cells , Humans , Immunoprecipitation , Madin Darby Canine Kidney Cells , Mice , Morphogenesis , Protein Binding , Transfection
10.
Acta Crystallogr Sect F Struct Biol Cryst Commun ; 68(Pt 12): 1550-3, 2012 Dec 01.
Article in English | MEDLINE | ID: mdl-23192045

ABSTRACT

TubZ is a structural homologue of tubulin and FtsZ GTPases, which are involved in the type III plasmid-partitioning system. TubZ assembles into polymers in a GTP-dependent manner and drives plasmid segregation as `cytomotive' filaments. In this study, C-terminally truncated TubZ from Bacillus cereus was crystallized in the presence or absence of GDP by the hanging-drop vapour-diffusion method. The crystal of TubZ in complex with GDP belonged to the monoclinic space group P2(1), with unit-cell parameters a=67.05, b=84.49, c=67.66 Å, ß=92.92°, and was non-isomorphous with GDP-bound TubZ previously crystallized in the presence of the slowly hydrolysable GTP analogue GTPγS. TubZ was also crystallized in the free form and the crystal belonged to space group P2(1), with unit-cell parameters a=53.91, b=65.54, c=58.18 Å, ß=106.19°. Data were collected to 1.7 and 2.1 Šresolution for the free and GDP-bound forms, respectively.


Subject(s)
Bacillus cereus/metabolism , Bacterial Proteins/chemistry , GTP Phosphohydrolases/chemistry , Bacterial Proteins/metabolism , Crystallization , Crystallography, X-Ray , GTP Phosphohydrolases/metabolism , Guanosine Triphosphate/chemistry , Guanosine Triphosphate/metabolism , Hydrolysis , Plasmids/chemistry , Plasmids/genetics , Tubulin/chemistry , Tubulin/metabolism
11.
J Biol Chem ; 287(38): 32103-12, 2012 Sep 14.
Article in English | MEDLINE | ID: mdl-22847006

ABSTRACT

Stable maintenance of low-copy-number plasmids requires partition (par) systems that consist of a nucleotide hydrolase, a DNA-binding protein, and a cis-acting DNA-binding site. The FtsZ/tubulin-like GTPase TubZ was identified as a partitioning factor of the virulence plasmids pBtoxis and pXO1 in Bacillus thuringiensis and Bacillus anthracis, respectively. TubZ exhibits high GTPase activity and assembles into polymers both in vivo and in vitro, and its "treadmilling" movement is required for plasmid stability in the cell. To investigate the molecular mechanism of pXO1 plasmid segregation by TubZ filaments, we determined the crystal structures of Bacillus cereus TubZ in apo-, GDP-, and guanosine 5'-3-O-(thio)triphosphate (GTPγS)-bound forms at resolutions of 2.1, 1.9, and 3.3 Å, respectively. Interestingly, the slowly hydrolyzable GTP analog GTPγS was hydrolyzed to GDP in the crystal. In the post-GTP hydrolysis state, GDP-bound B. cereus TubZ forms a dimer by the head-to-tail association of individual subunits in the asymmetric unit, which is similar to the protofilament formation of FtsZ and B. thuringiensis TubZ. However, the M loop interacts with the nucleotide-binding site of the adjacent subunit and stabilizes the filament structure in a different manner, which indicates that the molecular assembly of the TubZ-related par systems is not stringently conserved. Furthermore, we show that the C-terminal tail of TubZ is required for association with the DNA-binding protein TubR. Using a combination of crystallography, site-directed mutagenesis, and biochemical analysis, our results provide the structural basis of the TubZ polymer that may drive DNA segregation.


Subject(s)
Bacillus cereus/metabolism , Bacillus cereus/pathogenicity , Bacterial Proteins/chemistry , Cytoskeletal Proteins/chemistry , Plasmids/metabolism , Tubulin/chemistry , Binding Sites , Crystallography, X-Ray/methods , Dimerization , Hydrolysis , Molecular Conformation , Promoter Regions, Genetic , Protein Binding , Protein Conformation , Protein Structure, Tertiary , Virulence
12.
Exp Cell Res ; 318(3): 262-75, 2012 Feb 01.
Article in English | MEDLINE | ID: mdl-22134091

ABSTRACT

Mal3 is a fission yeast homolog of EB1, a plus-end tracking protein (+TIP). We have generated a mutation (89R) replacing glutamine with arginine in the calponin homology (CH) domain of Mal3. Analysis of the 89R mutant in vitro has revealed that the mutation confers a higher affinity to microtubules and enhances the intrinsic activity to promote the microtubule-assembly. The mutant Mal3 is no longer a +TIP, but binds strongly the microtubule lattice. Live cell imaging has revealed that while the wild type Mal3 proteins dissociate from the tip of the growing microtubules before the onset of shrinkage, the mutant Mal3 proteins persist on microtubules and reduces a rate of shrinkage after a longer pausing period. Consequently, the mutant Mal3 proteins cause abnormal elongation of microtubules composing the spindle and aster. Mal3 is phosphorylated at a cluster of serine/threonine residues in the linker connecting the CH and EB1-like C-terminal motif domains. The phosphorylation occurs in a microtubule-dependent manner and reduces the affinity of Mal3 to microtubules. We propose that because the 89R mutation is resistant to the effect of phosphorylation, it can associate persistently with microtubules and confers a stronger stability of microtubules likely by reinforcing the cylindrical structure.


Subject(s)
Microtubule-Associated Proteins/genetics , Microtubule-Associated Proteins/metabolism , Microtubules/metabolism , Mutation/physiology , Protein Kinases/metabolism , Schizosaccharomyces pombe Proteins/genetics , Schizosaccharomyces pombe Proteins/metabolism , Schizosaccharomyces/genetics , Amino Acid Sequence , Down-Regulation , Microtubules/chemistry , Models, Molecular , Molecular Sequence Data , Organisms, Genetically Modified , Phosphorylation/genetics , Protein Processing, Post-Translational , Protein Stability , Sequence Homology, Amino Acid
13.
J Am Chem Soc ; 130(45): 14918-9, 2008 Nov 12.
Article in English | MEDLINE | ID: mdl-18937471

ABSTRACT

XH/pi hydrogen bonds have been predicted to make important contributions to protein structure and function. NMR evidence is presented for an OH/pi interaction between a highly conserved threonine and phenylalanine pair found specifically in CAP-Gly domains associated with mictrotubule plus ends. The functional contribution of this nonclassical hydrogen bond in target peptide recognition is demonstrated via subtle point mutagenesis. The OH/pi interaction is part of a TxFxxxxW motif that comprises a conserved "threonine clasp" that defines function in CAP-Gly domains.


Subject(s)
Microtubule-Associated Proteins/chemistry , Neoplasm Proteins/chemistry , Threonine/chemistry , Dynactin Complex , Humans , Hydrogen Bonding , Models, Molecular , Nuclear Magnetic Resonance, Biomolecular/methods , Peptide Fragments/chemistry , Protein Structure, Secondary , Protein Structure, Tertiary
15.
Nat Struct Mol Biol ; 14(10): 980-1, 2007 Oct.
Article in English | MEDLINE | ID: mdl-17828275

ABSTRACT

CLIP170 and p150(Glued) localize to the plus ends of growing microtubules. Using crystallography and NMR, we show that autoinhibitory interactions within CLIP170 use the same binding determinants as CLIP170's intermolecular interactions with p150(Glued). These interactions have both similar and distinct features when compared with the p150(Glued)-EB1 complex. Our data thus demonstrate that regulation of microtubule dynamics by plus end-tracking proteins (+TIPs) occurs through direct competition between homologous binding interfaces.


Subject(s)
Microtubule-Associated Proteins/metabolism , Neoplasm Proteins/metabolism , Amino Acid Sequence , Animals , Dynactin Complex , Humans , Microtubule-Associated Proteins/genetics , Models, Molecular , Molecular Sequence Data , Neoplasm Proteins/genetics , Nuclear Magnetic Resonance, Biomolecular , Sequence Alignment
16.
J Mol Biol ; 372(4): 918-926, 2007 Sep 28.
Article in English | MEDLINE | ID: mdl-17692334

ABSTRACT

A coral fluorescent protein from Trachyphyllia geoffroyi, Kaede, possesses a tripeptide of His62-Tyr63-Gly64, which forms a chromophore with green fluorescence. This chromophore's fluorescence turns red following UV light irradiation. We have previously shown that such photoconversion is achieved by a formal beta-elimination reaction, which results in a cleavage of the peptide bond found between the amide nitrogen and the alpha-carbon at His62. However, the stereochemical arrangement of the chromophore and the precise structural basis for this reaction mechanism previously remained unknown. Here, we report the crystal structures of the green and red form of Kaede at 1.4 A and 1.6 A resolutions, respectively. Our structures depict the cleaved peptide bond in the red form. The chromophore conformations both in the green and red forms are similar, except a well-defined water molecule in the proximity of the His62 imidazole ring in the green form. We propose a molecular mechanism for green-to-red photoconversion, which is assisted by the water molecule.


Subject(s)
Anthozoa/chemistry , Light , Luminescent Proteins , Peptides/metabolism , Protein Structure, Quaternary , Water/chemistry , Animals , Crystallography, X-Ray , Green Fluorescent Proteins/chemistry , Green Fluorescent Proteins/metabolism , Luminescent Proteins/chemistry , Luminescent Proteins/metabolism , Models, Molecular , Molecular Sequence Data , Molecular Structure , Peptides/chemistry , Photochemistry
17.
Mol Cell ; 19(4): 449-60, 2005 Aug 19.
Article in English | MEDLINE | ID: mdl-16109370

ABSTRACT

Plus-end tracking proteins, such as EB1 and the dynein/dynactin complex, regulate microtubule dynamics. These proteins are thought to stabilize microtubules by forming a plus-end complex at microtubule growing ends with ill-defined mechanisms. Here we report the crystal structure of two plus-end complex components, the carboxy-terminal dimerization domain of EB1 and the microtubule binding (CAP-Gly) domain of the dynactin subunit p150Glued. Each molecule of the EB1 dimer contains two helices forming a conserved four-helix bundle, while also providing p150Glued binding sites in its flexible tail region. Combining crystallography, NMR, and mutational analyses, our studies reveal the critical interacting elements of both EB1 and p150Glued, whose mutation alters microtubule polymerization activity. Moreover, removal of the key flexible tail from EB1 activates microtubule assembly by EB1 alone, suggesting that the flexible tail negatively regulates EB1 activity. We, therefore, propose that EB1 possesses an auto-inhibited conformation, which is relieved by p150Glued as an allosteric activator.


Subject(s)
Microtubule-Associated Proteins/chemistry , Microtubules/metabolism , Base Sequence , Binding Sites , Crystallography, X-Ray , Dimerization , Dynactin Complex , Humans , Magnetic Resonance Spectroscopy , Microtubule-Associated Proteins/genetics , Microtubule-Associated Proteins/metabolism , Models, Biological , Models, Molecular , Molecular Sequence Data , Protein Structure, Tertiary/physiology
19.
J Biol Chem ; 278(38): 36430-4, 2003 Sep 19.
Article in English | MEDLINE | ID: mdl-12857735

ABSTRACT

The end-binding protein 1 (EB1) family is a highly conserved group of proteins that localizes to the plus-ends of microtubules. EB1 has been shown to play an important role in regulating microtubule dynamics and chromosome segregation, but its regulation mechanism is poorly understood. We have determined the 1.45-A resolution crystal structure of the amino-terminal domain of EB1, which is essential for microtubule binding, and show that it forms a calponin homology (CH) domain fold that is found in many proteins involved in the actin cytoskeleton. The functional CH domain for actin binding is a tandem pair, whereas EB1 is the first example of a single CH domain that can associate with the microtubule filament. Although our biochemical study shows that microtubule binding of EB1 is electrostatic in part, our mutational analysis suggests that the hydrophobic network, which is partially exposed in our crystal structure, is also important for the association. We propose that, like other actin-binding CH domains, EB1 employs the hydrophobic interaction to bind to microtubules.


Subject(s)
Microtubule-Associated Proteins/chemistry , Actins/chemistry , Amino Acid Sequence , Animals , Chromosomes/ultrastructure , Crystallography, X-Ray , Cytoskeleton/chemistry , Dose-Response Relationship, Drug , Humans , Microtubules/metabolism , Models, Molecular , Molecular Sequence Data , Protein Binding , Protein Folding , Protein Structure, Tertiary , Salts/pharmacology , Sequence Homology, Amino Acid , Sodium Chloride/pharmacology
20.
Nat Struct Biol ; 9(2): 101-6, 2002 Feb.
Article in English | MEDLINE | ID: mdl-11799401

ABSTRACT

Focal adhesion kinase (FAK) is a tyrosine kinase found in focal adhesions, intracellular signaling complexes that are formed following engagement of the extracellular matrix by integrins. The C-terminal 'focal adhesion targeting' (FAT) region is necessary and sufficient for localizing FAK to focal adhesions. We have determined the crystal structure of FAT and show that it forms a four-helix bundle that resembles those found in two other proteins involved in cell adhesion, alpha-catenin and vinculin. The binding of FAT to the focal adhesion protein, paxillin, requires the integrity of the helical bundle, whereas binding to another focal adhesion protein, talin, does not. We show by mutagenesis that paxillin binding involves two hydrophobic patches on opposite faces of the bundle and propose a model in which two LD motifs of paxillin adopt amphipathic helices that augment the hydrophobic core of FAT, creating a six-helix bundle.


Subject(s)
Cytoskeletal Proteins/chemistry , Cytoskeletal Proteins/metabolism , Focal Adhesions/chemistry , Focal Adhesions/metabolism , Phosphoproteins/chemistry , Phosphoproteins/metabolism , Protein-Tyrosine Kinases/chemistry , Protein-Tyrosine Kinases/metabolism , Amino Acid Motifs , Amino Acid Sequence , Animals , Binding Sites , Circular Dichroism , Crystallography, X-Ray , Focal Adhesion Kinase 1 , Focal Adhesion Protein-Tyrosine Kinases , Mice , Models, Molecular , Molecular Sequence Data , Mutation/genetics , Paxillin , Protein Binding , Protein Structure, Secondary , Protein Structure, Tertiary , Protein Transport , Protein-Tyrosine Kinases/genetics , Sequence Alignment
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