ABSTRACT
Genistein, kind of soy isoflavones, is well-known as natural ingredients and consumed as health foods and supplements. They are expected to improve renal function. They have high-affinity to estrogen receptor ß expressed predominantly in bone tissue, they prevent osteoporosis specifically and safely. We examined whether genistein can be a new direct capping agent. In this study, we examined the effect of genistein for the proliferation and differentiation of rat dental pulp cells in vitro and the ability of tertiary dentin formation in vivo. As a result, rat dental pulp cells with genistein were increased activity of ALPase and showed alizarin red positive-staining. Calcification-related genes expression has been confirmed by the addition of genistein. From in vivo study, high quality of tertiary dentin formation and minor pulp reaction were observed. From these findings, it was suggested that genistein may be useful agent for direct pulp capping.
Subject(s)
Genistein/administration & dosage , Pulp Capping and Pulpectomy Agents , Alkaline Phosphatase/metabolism , Animals , Base Sequence , Cell Differentiation , Cell Proliferation , DNA Primers , Dental Pulp/cytology , Dental Pulp/enzymology , Enzyme-Linked Immunosorbent Assay , Male , Osteocalcin/metabolism , Polymerase Chain Reaction , Protein-Tyrosine Kinases/metabolism , Rats , Rats, Wistar , p38 Mitogen-Activated Protein Kinases/metabolismABSTRACT
INTRODUCTION: In vital pulp therapy such as direct pulp capping, clinical success rates depend on achieving hemostasis in exposed pulp tissue. For hemostasis of exposed pulp tissue, gentle pressure by cotton pellets moistened with sodium hypochlorite is most commonly used. However, more rapid and reliable methods are necessary. Therefore, we focused on high-frequency radio waves (HRW). METHODS: To evaluate reparative dentin induction by HRW, we used a rat direct pulp capping model and performed hemostasis by using HRW of several strengths, covering the pulp with calcium hydroxide as a direct capping agent. After 14 or 28 days, rats were killed, and reparative dentin and pulp inflammation were investigated histologically. RESULTS: Radio wave-induced hemostasis required less time when compared with the control group. Reparative dentin with regularly arranged dentinal tubules was observed in the HRW group. CONCLUSIONS: HRW induce hemostasis and produce high-quality reparative dentin and reduced pulpal inflammation.
Subject(s)
Dental Pulp Capping/methods , Hemostatic Techniques , Radiofrequency Therapy , Animals , Calcium Hydroxide/therapeutic use , Dental Pulp Exposure/drug therapy , Dental Pulp Exposure/radiotherapy , Dentin, Secondary/drug effects , Dentin, Secondary/pathology , Dentin, Secondary/radiation effects , Hydrogen Peroxide/therapeutic use , Male , Models, Animal , Odontoblasts/drug effects , Odontoblasts/pathology , Odontoblasts/radiation effects , Pulp Capping and Pulpectomy Agents/therapeutic use , Pulpitis/classification , Pulpitis/prevention & control , Rats , Rats, Wistar , Sodium Hypochlorite/therapeutic use , Time FactorsABSTRACT
The aim of this study was to clarify the function of amelogenin, the major protein of enamel matrix derivative, on the proliferation, differentiation, and mineralization of cultured rat bone marrow stem cells (BMSCs), toward the establishment of future bone regenerative therapies. No differences in the morphology of BMSCs or in cell numbers were found between amelogenin addition and additive-free groups. The promotion of ALPase activity and the formation of mineralized nodules were detected at an early stage in amelogenin addition group. In quantitative real-time RT-PCR, mRNA expression of osteopontin, osteonectin, and type I collagen was promoted for 0.5 hours and 24 hours by addition of amelogenin. The mRNA expression of osteocalcin and DMP-1 was also stimulated for 24 hours and 0.5 hours, respectively, in amelogenin addition group. These findings clearly indicate that amelogenin promoted the differentiation and mineralization of rat BMSCs but did not affect cell proliferation or cell morphology.
